A5006759540- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Retinal Imaging and Analysis
- Retinal and Optic Conditions
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- Glaucoma and retinal disorders
- CRISPR and Genetic Engineering
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neural Engineering
- Laser Applications in Dentistry and Medicine
- Pluripotent Stem Cells Research
- Retinopathy of Prematurity Studies
- Insect and Arachnid Ecology and Behavior
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Axon Guidance and Neuronal Signaling
- Insect and Pesticide Research
- Retinal and Macular Surgery
- Plant and animal studies
- Circadian rhythm and melatonin
- Angiogenesis and VEGF in Cancer
- Cellular transport and secretion
- Immune cells in cancer
- Advanced Chemical Sensor Technologies
St George's, University of London
2025
City, University of London
2016-2024
University College London
2010-2023
University of London
2023
Northampton Community College
2021
Prevent Blindness
2014
Institute of Ophthalmology
2014
University College Hospital
2014
UCL Biomedical Research Centre
2012
Purpose.: To investigate the impact of diabetic macular ischemia (DMI) on visual acuity (VA), through analysis novel fluorescein angiography (FA) parameters. Methods.: Data were retrospectively collected over a 6-month period. DMI severity was graded using Early Treatment Diabetic Retinopathy Study (ETDRS) protocols. Custom software used to quantify areas foveal avascular zone (FAZ), and capillary nonperfusion papillomacular nerve fiber layer bundle, temporal macula, associations tested with...
Inflammation is an umbrella feature of ageing. It present in the aged retina and many retinal diseases including age-related macular degeneration (AMD). In ageing AMD mitochondrial function declines. normal this can be manipulated by brief exposure to 670 nm light on retina, which increases membrane potential reduces inflammation. Here we ask if has same ability mouse model AMD, complement factor H knockout (CFH−/−) where inflammation a key feature. Further, whether occurs when delivered...
Vascular endothelial growth factor (VEGF) plays a critical role in normal development as well retinal vasculature disease. During vascularization, VEGF is most strongly expressed by not yet vascularized astrocytes, but also astrocytes within the developing vascular plexus, suggesting for astrocyte-derived angiogenesis and vessel network maturation. To test of VEGF, we used Cre-lox technology mice to delete during development. Surprisingly, this only had minor impact on development, with...
Highlights•At blood-neural barriers, only abluminal (tissue-side) VEGF-A induces permeability•Most VEGFR1 is localized on the luminal face of neural microvascular endothelium•Most VEGFR2 endothelium•Luminal stimulates Akt; permeability via p38SummaryThe vascular endothelium operates in a highly polarized environment, but to date there has been little exploration apicobasal polarization its signaling. We show that VEGF-A, histamine, IGFBP3, and LPA trigger unequal endothelial responses when...
Abstract Mitochondria regulate metabolism, but solar light influences its rate. Photobiomodulation (PBM) with red (670 nm) increases mitochondrial membrane potentials and adenosine triphosphate production may increase glucose demand. Here we show, a tolerance test, that PBM of normal subjects significantly reduces blood sugar levels. A 15 min exposure to 670 nm reduced the degree elevation following intake by 27.7%, integrated over 2 h after challenge. Maximum spiking was 7.5%. Consequently,...
The neonatal Fc receptor (FcRn) plays a critical role in the homeostasis and degradation of immunoglobulin G (IgG). It mediates transport IgG across epithelial cell barriers recycles endothelial cells back into bloodstream. These functions critically depend on binding FcRn to domain IgG. half-life distribution intravitreally injected anti-VEGF molecules containing IgG-Fc domains might therefore be affected by expressed eye. In order establish whether FcRn-Fc(IgG) interactions may occur eye,...
Inherited retinal dystrophies are an important cause of blindness, for which currently there no effective treatments. In order to study this heterogeneous group diseases, adequate disease models required in better understand pathology and test potential therapies. Induced pluripotent stem cells offer a new way recapitulate patient specific diseases vitro, providing almost limitless amount material study. We used fibroblast-derived induced generate pigment epithelium (RPE) from individual...
Mitochondria absorb short wavelengths around 420 nm. This is associated with reduced ATP and restricted mobility. The 420–450 nm range a significant element of LED lighting computer monitors. Here we expose freely moving mice to show rapidly weight gain within week. may be due mitochondrial demand for circulating carbohydrates. Both groups displayed marked shifts in serum cytokines. Open field mobility was examined. distance travelled similar between both experimental their controls....
Differential cell death is a common feature of aging and age-related disease. In the retina, 30% rod photoreceptors are lost over life in humans rodents. However, studies have failed to show mouse cone photoreceptors, which surprising because physiological function declines with age. Moreover human, differential loss short wavelength an aspect retinal Here, cones examined young (3-month-old) aged (12-month-old) C57 mice also complement factor H knock out (CFH-/-) that been proposed as murine...
Global pollination is threatened by declining insect pollinator populations that may be linked to neonicotinoid pesticide use. Neonicotinoids over stimulate neurons and depolarize their mitochondria, producing immobility death. However, mitochondrial function can improved near infrared light absorbed cytochrome c oxidase in respiration. In flies, daily exposure 670nm throughout life increases average lifespan aged mobility, reduces systemic inflammation. Here we treat bumble bees with...
Retinal ischemia has been traditionally assessed by fluorescein angiography, visualizing perfused vessels. However, this method does not provide any information about nonperfused vessels, and although it is often assumed that vessels in ischemic areas regress, we know little how retinal change over time. Here, aim to learn more the long-term fate of vasculature.Optical coherence tomography (OCT) was used visualize perfusion as well structural properties vasculature patients suffering from...
Autosomal dominant vitreoretinochoroidopathy (ADVIRC) is a rare, early-onset retinal dystrophy characterised by distinct bands of circumferential pigmentary degeneration in the peripheral retina and developmental eye defects. ADVIRC caused mutations Bestrophin1 (BEST1) gene, which encodes transmembrane protein thought to function as an ion channel basolateral membrane pigment epithelial (RPE) cells. Previous studies suggest that phenotype results from alternative splicing BEST1 pre-mRNA....
Abstract Vertebrate photoreceptors contain large numbers of closely-packed mitochondria which sustain the high metabolic demands these cells. These populations are dynamic and undergo fusion fission events. This activity serves to maintain population in a healthy state. In event mitochondrial damage, sub-domains, or indeed whole mitochondria, can be degraded homeostasis achieved. If this process is overwhelmed cell death may result. Death contributes loss vision aging individuals associated...
We used the comparative proteomic technique iTRAQ coupled with offline 2DLC-MS/MS to analyze a rare specimen of poorly understood, potentially blinding ophthalmic condition Macular Telangiectasia type 2 (MacTel 2). refined using an internal standard consisting pooled samples for each experiment allow multiple comparisons between different regions retina and tissue donors. A total 594 nonredundant proteins were identified in 168 vitreous, which approximately half found significantly abundance...
Molecular oxygen is essential for the development, growth and survival of multicellular organisms. Hypoxic microenvironments gradients are generated physiologically during embryogenesis organogenesis. In eye, plays a crucial role in both physiological vascular development common blinding diseases. The retinal pigment epithelium (RPE) monolayer cells normal ocular mature retina provides support overlying photoreceptors their supply. Hypoxia at level RPE closely implicated pathogenesis...