Novel surface-imprinted conducting polymer microrods are shown to selectively recognize the template protein, as demonstrated by competitive binding assays using fluorescence detection. The electrochemical synthesis provides means for controlled fabrication and spatial confinement of PEDOT/PSS proposed in this work, which exhibits extraordinary low nonspecific interactions is effectively turned into a selective protein sorbent upon imprinting.

Two molecularly imprinted polymers were synthesized using either dichloromethane or toluene as the porogen and terbuthylazine template used solid-phase extraction cartridges for enrichment of six chlorotriazines (deisopropylatrazine, deethylatrazine, simazine, atrazine, propazine, terbuthylazine) in natural water sediment samples. The extracted samples analyzed by liquid chromatography/diode array detection (LC/DAD). Several washing solvents, well different volumes, tested their ability to...

Abstract A versatile approach based on nanosphere lithography is proposed to generate surface‐imprinted polymers for selective protein recognition. layer of 750 nm diameter latex bead‐protein conjugate deposited onto the surface gold‐coated quartz crystals followed by electrosynthesis a poly(3,4‐ethylenedioxythiophene)/poly(styrenesulfonate) (PEDOT/PSS) film with thicknesses order bead radius. The removal polymer conjugates, facilitated using cleavable protein‐nanosphere linkage shown result...

We have developed a liposome-based flow injection immunoassay (FIIA) system for quantitation of clinical analyte, theophylline. With very minor changes in assay format, this procedure can also be used the anti-theophylline. Automated sequential analyses were performed at room temperature with picomole sensitivity and day-to-day coefficient variation less than 5% aqueous solutions. The components include liposomes that contain fluorophores their centers an immobilized-antibody reactor column....

Nanosphere lithography with nanosphere-protein conjugates is proposed by R. E. Gyurcsányi and co-workers as a generic method to create surface molecularly imprinted polymer films for selective protein recognition. On page 4703, electrosynthesis of film in the voids nanosphere array followed removal nanospheres generates complementary cavities bearing imprints proteins.

A versatile approach for the preparation of photoswitchable molecularly imprinted polymers (MIPs) is proposed where selective recognition and photoresponsive function are assumed by two different monomers. As a proof concept, MIP microspheres were synthesized precipitation polymerization recognizing terbutylazine, triazine-type herbicide. Formation binding sites was based upon H-bonding interactions between template functional monomer methacrylic acid, whereas polymerizable spiropyran unit...

The host-guest complex of the anionic macrocycle, carboxylato-pillar[6]arene (WP6) and cationic dye, oxazine 1 (OX), functions as a fluorescent turn-on indicator displacement assay (IDA) for biomolecule, vitamin B1 (thiamine). performance was demonstrated by applying it in analysis poly B supplement. structures OX-WP6 B1-WP6 complexes were characterized 1H NMR spectroscopy theoretical calculations. To our knowledge, this is first report on construction an IDA-based sensor B1.

Lead is a particularly toxic heavy metal that present above acceptable levels in the water of many countries. This article describes quick detection method lead(II) ions using polyvinyl chloride (PVC)-based ion-selective membrane electrode containing an acridono-crown ether ionophore by potentiometry. The electrochemical cell exhibits Nernstian response for between concentration range 10−4 to 10−2 M, and can be used pH 4–7. applicability this sensor was verified measuring multicomponent...

Abstract A newly synthesized crown ether ionophore can differentiate between R-(+) and S-(-)-1-phenylethylammonium ions when incorporated into a plasticized PVC based electrode membrane, thus providing enantiomer-selectivity. Different membrane compositions were studied. The selectivity coefficient found was 0.71, independent of the composition. This proves robustness ISE method for testing enantiomeric ionophores. Selectivities new towards different hydrophilic cations also determined. Key...

A novel technique for the synthesis and testing of large numbers molecularly imprinted polymers is described requiring much less time than commonly used miniMIP approach. Instead vials, are synthesized on surface microfiltration membranes in multiwell filterplates. The thin polymeric films enable accelerated template removal. MIP development procedure thereby shortened to two days. Performance system was demonstrated by creating a combinatorial library MIPs selective cimetidine, an antiulcer...

Abstract Highly crosslinked polymer microparticles have been prepared by precipitation polymerization using high monomer loadings (≥25 v/v %) which generally would lead to bulk monoliths. The microparticle format was achieved the use of non-solvating diluents either alone or in combination with co-solvents. Two distinct morphologies were observed. Monodisperse smooth microspheres obtained a thermodynamically good co-solvent whereas segmented irregular particles formed poorer It has found...

Molecularly imprinted polymers (MIPs) were synthesized in 24-well glass fiber membrane filter plates to obtain a novel type of solid phase extraction device for the cleanup propranolol. Sample processing parameters like residence time during sample loading, volume, pH, solvent, and amount washing elution solvents investigated optimized. Important differences from traditional molecularly (MISPE) cartridges have been identified. The MIP modified composite suits well preparation low volume...

There is a growing need in membrane separations for novel materials providing selective retention. Molecularly imprinted polymers (MIPs) are promising candidates functionalization. In this work, approach described to prepare composite adsorbers incorporating molecularly microparticles or nanoparticles into commercially available macroporous filtration membranes. The polymerization carried out highly viscous solvents, and the particles formed situ pores of support membrane. MIP particle...

Using long-lifetime fluorophores as protein labels, protein–nanoparticle interactions can be monitored through anisotropy change. Besides gaining thermodynamic and kinetic information on the binding process, competitive assays set up.

To date, numerous aptamer-based biosensing platforms have been developed for sensitive and selective monitoring of target analytes, relying on analyte-induced conformational changes in the aptamer quantification analyte conversion binding event into a measurable signal. Despite impact these rearrangements sensor performance, influence environment structural conformations aptamers has rarely investigated, so link between parameters directly influencing folding ability to bind remains elusive....

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLiposome flow injection immunoassay: model calculations of competitive immunoreactions involving univalent and multivalent ligandsWilliam T. Yap, Laurie. Locascio-Brown, Anne L. Plant, Steven J. Choquette, Viola. Horvath, Richard A. DurstCite this: Anal. Chem. 1991, 63, 18, 2007–2011Publication Date (Print):September 15, 1991Publication History Published online1 May 2002Published inissue 15 September...