A5061650627- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- Pneumocystis jirovecii pneumonia detection and treatment
- Bacterial Infections and Vaccines
- Streptococcal Infections and Treatments
- SARS-CoV-2 and COVID-19 Research
- Neonatal and Maternal Infections
- Antibiotic Resistance in Bacteria
- Viral gastroenteritis research and epidemiology
- Urinary Tract Infections Management
- Food Security and Health in Diverse Populations
- COVID-19 epidemiological studies
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Use and Resistance
- Child Nutrition and Water Access
- Sepsis Diagnosis and Treatment
- Otolaryngology and Infectious Diseases
- Adolescent Sexual and Reproductive Health
- Emergency and Acute Care Studies
- SARS-CoV-2 detection and testing
- Infant Development and Preterm Care
- Global Maternal and Child Health
- Bacteriophages and microbial interactions
- Pharmaceutical and Antibiotic Environmental Impacts
- Biosensors and Analytical Detection
Armauer Hansen Research Institute
2019-2025
Addis Ababa University
2021-2025
Wellcome Sanger Institute
2025
World Health Organization Regional Office for Africa
2022
Ghent University
2019
Although evidence exists on the impact of microbiota pregnancy outcomes in many high-resource settings, there is a lack research low-resource settings like Ethiopia. This study aims to fill this gap by studying gut and vaginal changes throughout assess how these relate among cohort pregnant women eastern Vaginal stool samples will be collected using DNA/RNA Shield Collection kits three times starting at 12-22 weeks, 28-36 weeks birth (within 7 days). Postnatally, newborns' skin swabs (at...
Background and aims. Despite the introduction of pneumococcal conjugate vaccines (PCVs), Streptococcus pneumoniae still remains an important cause morbidity mortality, especially among children under 5 years in sub-Saharan Africa. We sought to determine distribution serotypes, lineages antimicrobial resistance S. from carriage disease presenting health facilities, 5–6 after PCV10 Ethiopia. Methods. Whole-genome sequencing (WGS) was performed on 103 (86 nasopharyngeal swabs, 4 blood 13 middle...
Abstract Background Community-acquired pneumonia (CAP) remains a leading cause of morbidity and mortality. We sought to determine the magnitude, etiology, risk factors CAP in children 5 years after introduction pneumococcal conjugate vaccine (PCV) 10 Ethiopia. Methods conducted prospective observational study on bacterial etiology among aged 0–15 2 pediatric emergency departments Addis Ababa, Blood culture, antibiotic susceptibility testing, amplification lytA cpsB genes were performed....
Globally, extended-spectrum beta-lactamase-producing and carbapenem-resistant Enterobacterales are major causes of hospital-acquired infections there increasing concerns about their role in community-acquired infections.
There is a scarcity of data on pneumococcal serotypes carried by children in Ethiopia. We studied nasopharyngeal carriage rate, serotypes, and risk factors among with community acquired pneumonia (CAP). A prospective observational cohort study was performed CAP, aged 0-15 years, 2 pediatric emergency departments Addis Ababa, Nasopharyngeal swabs were cultured, Streptococcus pneumoniae determined sequencing the cpsB gene Quellung reaction. Risk analyzed using binary logistic regression....
Accurate DNA quantification is key for downstream application including library preparations whole genome sequencing (WGS) and the of standards quantitative PCR. Two commonly used technologies nucleic acid are based on spectrometry, such as NanoDrop, fluorometry, Qubit. The DS–11+ Series spectrophotometer/fluorometer (DeNovix) a UV spectrophotometry-based instrument relatively new spectrophotometric method but has not yet been compared to established platforms. Here, we three platforms, two...
Staphylococcus aureus and Streptococcus pneumoniae are common inhabitants of the nasopharynx children. HIV-infected children have higher risk invasive diseases caused by these pathogens. With widespread use pneumococcal conjugate vaccines emergence methicillin-resistant S. , interaction between is a particular significance. We sought to determine magnitude colonization methicillin-sensitive -resistant ; associated factors antimicrobial susceptibility pattern among in Addis Ababa, Ethiopia.A...
In several countries, introduction of the pneumococcal conjugate vaccine (PCV) has led to a decline in antimicrobial-resistant disease but also resulted concomitant increase antimicrobial-resistant, non-vaccine serotypes Streptococcus pneumoniae. We sought determine magnitude penicillin and macrolide resistance among mechanisms Ethiopia, 5 years after PCV10 country.Susceptibility erythromycin 119 isolates collected from pediatric patients aged 0-15 Addis Ababa, was tested using disc...
The antimicrobial resistance catastrophe is a growing global health threat and predicted to be worse in developing countries. Phages for Global Health (PGH) training scientists these regions isolate relevant therapeutic phages pathogenic bacteria within their locality, thus contributing making phage technology universally available.During the inaugural PGH workshop East Africa, samples from Ugandan municipal sewage facilities were collected two novel Escherichia coli lytic isolated...
Introduction: There is a scarcity of data on pediatric community-acquired sepsis (CAS) in Ethiopia. We sought to determine the etiology, role Streptococcus pneumoniae, antibiotic susceptibility pattern, and prognostic factors children with CAS Addis Ababa, Ethiopia.
 Methodology: A prospective cross-sectional study 101 aged 0-15 years suspected was performed at two major hospitals Blood culture, testing, amplification autolysin (lytA) gene typing S. pneumoniae by sequencing Quellung...
Abstract Background and aims Despite the introduction of pneumococcal conjugate vaccines (PCV), Streptococcus pneumoniae still remains an important cause morbidity mortality, especially among children under 5 years in sub-Saharan Africa. We sought to determine distribution lineages antimicrobial resistance genes S. , 5-6 after PCV10 Ethiopia. Methods Whole genome sequencing (WGS) was performed on 103 (86 from nasopharyngeal swabs, 4 blood 13 middle ear swabs) isolated aged < 15 at three...
is the most frequent cause of pharyngitis and skin infections in children causes immune complications like rheumatic fever rheumatoid heart disease (RHD), particularly developing countries Ethiopia. The aim this study was to determine prevalence, antibiotic resistance pattern, associated factors
Klebsiella bacterial strains harboring Extended-Spectrum Beta-Lactamase (ESBL) enzymes are the primary culprits behind neonatal sepsis globally. These significantly impact clinical outcomes due to their multi-drug resistance patterns in local healthcare settings. In response this spiraling threat, we studied prevalence and implications of ESBL-encoding genes neonates hospitalized with confirmed sepsis. A correlational study was conducted on 51 diagnosed caused by ESBL-positive pneumoniae at...
Abstract Background : There is a scarcity of data on pediatric community-acquired sepsis (CAS) in Ethiopia. We sought to determine the etiology, antibiotic susceptibility pattern, and prognostic factors children with CAS Addis Ababa, Methods A prospective cross-sectional study 101 aged 0-15 yrs suspected was performed. Blood culture, testing, Polymerase Chain Reaction (PCR) autolysin ( lyt A) gene from whole blood samples typing Streptococcus pneumoniae by sequencing cps B using Quellung...
Three complete severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genomes from Ethiopian patients were compared with deposited global genomes. Two belonged to genetic group 20A/B.1/GH, and the other 20A/B.1.480/GH. Enhancing genomic capacity is important investigate transmission monitor evolution mutational patterns of SARS-CoV-2 in this country.
Background Staphylococcus aureus and Streptococcus pneumoniae are common inhabitants of the nasopharynx children. HIV-infected children have higher risk invasive diseases caused by these pathogens. With widespread use pneumococcal conjugate vaccines emergence methicillin-resistant S aureus, interaction between S. is a particular significance. We sought to determine magnitude colonization methicillin-sensitive -resistant pneumoniae; associated factors antimicrobial susceptibility pattern...
Background Staphylococcus aureus and Streptococcus pneumoniae are common inhabitants of the nasopharynx children. HIV-infected children have higher risk invasive diseases caused by these pathogens. With widespread use pneumococcal conjugate vaccines emergence methicillin-resistant S aureus, interaction between S. is a particular significance. We sought to determine magnitude colonization methicillin-sensitive -resistant pneumoniae; associated factors antimicrobial susceptibility pattern...
Abstract Background Staphylococcus aureus and Streptococcus pneumoniae are common inhabitants of the nasopharynx children. HIV-infected children have higher risk invasive diseases caused by these pathogens. With widespread use pneumococcal conjugate vaccines emergence methicillin-resistant S , interaction between S. is a particular significance. We sought to determine magnitude colonization methicillin-sensitive -resistant ; associated factors antimicrobial susceptibility pattern among...