A5058277866- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- RNA Research and Splicing
- Peptidase Inhibition and Analysis
- Neonatal and Maternal Infections
- RNA regulation and disease
- Antimicrobial Resistance in Staphylococcus
- Streptococcal Infections and Treatments
- Cancer-related molecular mechanisms research
- Bacteriophages and microbial interactions
- Infective Endocarditis Diagnosis and Management
- Genetics, Bioinformatics, and Biomedical Research
- Cardiac Structural Anomalies and Repair
- Parasitic Infections and Diagnostics
- bioluminescence and chemiluminescence research
- CRISPR and Genetic Engineering
University of Florida
2015-2025
The Ohio State University
2014-2019
Threonylcarbamoyladenosine (t(6)A) is a modified nucleoside universally conserved in tRNAs all three kingdoms of life. The recently discovered genes for t(6)A synthesis, including tsaC and tsaD, are essential model prokaryotes but not yeast. These had been identified as antibacterial targets even before their functions were known. However, the molecular basis this prokaryotic-specific essentiality has remained mystery. Here, we show that strong positive determinant aminoacylation tRNA by...
Modifications found in the Anticodon Stem Loop (ASL) of tRNAs play important roles regulating translational speed and accuracy. Threonylcarbamoyl adenosine (t6A37) 5-methoxycarbonyl methyl-2-thiouridine (mcm5s2U34) are critical ASL modifications that have been linked to several human diseases. The model yeast Saccharomyces cerevisiae is viable despite absence both modifications, growth however greatly impaired. major observed consequence a subsequent increase protein aggregates aberrant...
N 6 -threonylcarbamoyl adenosine (t A) is a nucleoside modification found in all kingdoms of life at position 37 tRNAs decoding ANN codons, which functions part to restrict translation initiation AUG and suppress frameshifting tandem codons. In Bacteria the proteins TsaB, TsaC (or C2), TsaD, TsaE, comprise biosynthetic apparatus responsible for t A formation. TsaC(C2) TsaD harbor relevant active sites, with catalyzing formation intermediate threonylcarbamoyladenosine monophosphate (TC-AMP)...
Endoribonuclease toxins (ribotoxins) are produced by bacteria and fungi to respond stress, eliminate non-self competitor species, or interdict virus infection. PrrC is a bacterial ribotoxin that targets cleaves tRNALys UUU in the anticodon loop. In vitro studies suggested post-transcriptional modification threonylcarbamoyl adenosine (t6A) required for activity but this prediction had never been validated vivo. Here, using t6A-deficient yeast derivatives, it shown t6A positive determinant...
Accuracy in protein biosynthesis is maintained through multiple pathways, with a critical checkpoint occurring at the tRNA aminoacylation step catalyzed by aminoacyl-tRNA synthetases (ARSs). In addition to editing functions inherent some synthetases, single-domain trans-editing factors, which are structurally homologous ARS domains, have evolved as alternative mechanisms correct mistakes synthesis. To date, ARS-like domains been shown act on specific tRNAs that mischarged genetically encoded...
Abstract Current next-generation RNA sequencing methods cannot provide accurate quantification of the population small RNAs within a sample due to strong sequence-dependent biases in capture, ligation, and amplification during library preparation. We report development an method – AQRNA-seq that minimizes enables absolute all species mixture. Validation preparation data mining algorithms using 963-member microRNA reference library, oligonucleotide standards varying lengths, northern blots...
regulating translational speed and accuracy. Threonylcarbamoyl adenosine (t6A37) 5-methoxycarbonylmethyl-thiouridine (mcm5s2U34) are critical ASL modifications that have been linked to several human diseases. The model yeast Saccharomyces cerevisiae is viable despite the absence of both modifications, growth however greatly impaired. major observed consequence a subsequent increase in protein aggregates aberrant morphology. Proteomic analysis t6A-deficient strain revealed global...
Queuosine (Q) is a modification of the wobble base in tRNAs that decode NAC codons. It ubiquitous bacteria, including many pathogens. Streptococcus mutans an early colonizer dental plaque biofilm and key player caries. Using combination genetic physiological approaches, predicted Q synthesis salvage pathways were validated this organism. These experiments confirmed S. can synthesize de novo through similar found Bacillus subtilis Escherichia coli . However, has distinct pathway compared to...
tRNAs are the central adaptor molecule in translation and require a wide variety of post-transcriptional modifications to fulfill their functions. The model gram-negative
ABSTRACT Queuosine (Q) is a modification of the wobble base in tRNAs that decode NA(C/U) codons. It ubiquitous bacteria, including many pathogens. Streptococcus mutans an early colonizer dental plaque biofilm and key player caries. Using combination genetic physiological approaches, predicted Q synthesis salvage pathways were validated this organism. These experiments confirmed S. can synthesize de novo through similar found Bacillus subtilis Escherichia coli . However, has distinct pathway...
Bioinformatics, the study of biological data using various computational techniques, is a very important aspect biology, and its integration would greatly benefit current high school curricula. However, because most bioinformatics tools have not been readily accessible until recently, instructors were exposed to them during their formative years. We describe bioinformatics-based module that introduces application genome comparison in identification “pathogenic islands.” The also foundational...
Accurate translation of genetic information is determined, in part, by aminoacyl-tRNA synthetases (ARSs), which are responsible for the correct pairing amino acids with their cognate tRNA adaptors. However, these reactions susceptible to errors due structural similarities that challenge specificity some and promote incorporation at wrong codons during protein synthesis (mistranslation). To maintain translational fidelity, many have expanded aminoacylation capabilities include editing...