A5067348404- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- Streptococcal Infections and Treatments
- Analytical Chemistry and Chromatography
- Cancer-related gene regulation
- Neonatal and Maternal Infections
- Antimicrobial Resistance in Staphylococcus
- Cancer-related molecular mechanisms research
- Genomics and Phylogenetic Studies
- Microfluidic and Capillary Electrophoresis Applications
- Fungal Infections and Studies
Eli Lilly (United States)
2023-2025
University of Cincinnati
2016-2022
Retrograde transport of tRNAs from the cytoplasm to nucleus was first described in Saccharomyces cerevisiae and most recently mammalian systems. Although function retrograde is not completely clear, it plays a role cellular response changes nutrient availability. Under low conditions are sent presumably remain storage there until levels improve. However, S. tRNA constitutive occurs even when adequate. Constitutive important, at least, for proper maturation tRNAPhe, which undergoes...
Active tRNAs are extensively post-transcriptionally modified, particularly at the wobble position 34 and 37 on 3'-side of anticodon. The 5-carboxy-methoxy modification U34 (cmo5U34) is present in Gram-negative for six amino acids (Ala, Ser, Pro, Thr, Leu Val), four which Pro Thr) have a terminal methyl group to form 5-methoxy-carbonyl-methoxy-uridine (mcmo5U34) higher reading-frame accuracy. molecular basis selective methylation not understood. Many cmo5U34-tRNAs essential growth cannot be...
Even with the advent of high throughput methods to detect modified ribonucleic acids (RNAs), mass spectrometry remains a reliable method detect, characterize, and place post-transcriptional modifications within an RNA sequence. Here we have developed stable isotope labeling comparative analysis digests (SIL-CARD) approach, which improves upon original 18O/16O CARD method. Like original, SIL-CARD allows sequence or modification information from previously uncharacterized in vivo sample be...
Endoribonuclease toxins (ribotoxins) are produced by bacteria and fungi to respond stress, eliminate non-self competitor species, or interdict virus infection. PrrC is a bacterial ribotoxin that targets cleaves tRNALys UUU in the anticodon loop. In vitro studies suggested post-transcriptional modification threonylcarbamoyl adenosine (t6A) required for activity but this prediction had never been validated vivo. Here, using t6A-deficient yeast derivatives, it shown t6A positive determinant...
Cryptococcus neoformans is a fungus that able to survive abnormally high levels of ionizing radiation (IR). The radiolysis water by IR generates reactive oxygen species (ROS) such as H 2 O and OH - . C withstands the damage caused ROS through antioxidant production enzyme-catalyzed breakdown ROS. Given these particular cellular protein needs, questions arise whether transfer ribonucleic acids molecules (tRNAs) undergo unique chemical modifications maintain their structure, stability, and/or...
While a number of approaches have been developed to analyze liquid chromatography tandem mass spectrometry (LC-MS/MS) data obtained from modified oligonucleotides, the majority these methods require analyzing every MS/MS spectrum de novo sequence oligonucleotide and place modification. Spectral matching is an alternative approach for that based on creating library annotated spectra against which individual can be searched. Here, we adapted existing NIST spectral software enable its use in...