A5048808761- Fungal and yeast genetics research
- Plant-Microbe Interactions and Immunity
- RNA modifications and cancer
- Plant Disease Resistance and Genetics
- Nematode management and characterization studies
- Plant nutrient uptake and metabolism
- Molecular Biology Techniques and Applications
- Single-cell and spatial transcriptomics
- Genomics and Phylogenetic Studies
- Insect symbiosis and bacterial influences
- Chromosomal and Genetic Variations
- Cell Image Analysis Techniques
- Plant Molecular Biology Research
- Genomics and Chromatin Dynamics
- Gene expression and cancer classification
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Plant Gene Expression Analysis
- Plant Virus Research Studies
University of Edinburgh
2019-2024
Wellcome Centre for Cell Biology
2019-2024
Cancer Research UK
2024
University of Cambridge
2024
Institute of Cell Biology
2019-2021
Graduate School Experimental Plant Sciences
2017
Root growth is modulated by environmental factors and depends on cell production in the root meristem (RM). New cells are generated stem transit-amplifying cells, which together determine RM number. Transcription chromatin-remodeling have been implicated regulating switch from to cells. Here, we show that two Arabidopsis thaliana paralogs encoding plant-specific histone deacetylases, HDT1 HDT2, regulate a second expanding Knockdown of HDT1/2 (hdt1,2i) results an earlier causes reduced Our...
The CRISPR/Cas9 system allows scarless, marker-free genome editing. Current systems for the fission yeast Schizosaccharomyces pombe rely on tedious and time-consuming cloning procedures to introduce a specific sgRNA target sequence into Cas9-expressing plasmid. In addition, Cas9 endonuclease has been reported be toxic when constitutively overexpressed from strong adh1 promoter. To overcome these problems we have developed an improved system, SpEDIT, that uses synthesised codon-optimised S....
Abstract Despite the conserved essential function of centromeres, centromeric DNA itself is not conserved. The histone-H3 variant, CENP-A, epigenetic mark that specifies centromere identity. Paradoxically, CENP-A normally assembles on particular sequences at specific genomic locations. To gain insight into specification complex here we take an evolutionary approach, fully assembling genomes and centromeres related fission yeasts. Centromere domain organization, but sequence, between...
Abstract Mapping the molecular identities and functions of cells within their spatial context is key to understanding complex interplay between tissue neighbourhoods. A wide range methods have recently enabled profiling cellular anatomical contexts, some offering single-cell resolution. These use different barcoding schemes encode either location or identity target molecules. However, all these technologies face a trade-off resolution, depth profiling, scalability. Here, we present B...
Summary Genes embedded in H3 lysine 9 methylation (H3K9me)–dependent heterochromatin are transcriptionally silenced 1-3 . In fission yeast, Schizosaccharomyces pombe , H3K9me silencing can be transmitted through cell division provided the counteracting demethylase Epe1 is absent 4,5 It possible that under certain conditions wild-type cells might utilize heritability to form epimutations, phenotypes mediated by unstable rather than changes DNA 6,7 Here we show resistant...
Abstract Epe1 histone demethylase restricts H3K9-methylation-dependent heterochromatin, preventing it from spreading over, and silencing, gene-containing regions in fission yeast. External stress induces an adaptive response allowing heterochromatin island formation that confers resistance on surviving wild-type lineages. Here we investigate the mechanism by which is regulated to stress. Exposure caffeine or antifungals results ubiquitylation proteasome-dependent removal of N-terminal 150...