A5006927872- Prostate Cancer Diagnosis and Treatment
- Calcium signaling and nucleotide metabolism
- Adenosine and Purinergic Signaling
- Occupational and environmental lung diseases
- Air Quality and Health Impacts
- Phytase and its Applications
- MRI in cancer diagnosis
- Radiomics and Machine Learning in Medical Imaging
- Prostate Cancer Treatment and Research
- Ion Channels and Receptors
- Trace Elements in Health
- Burn Injury Management and Outcomes
- Thyroid Disorders and Treatments
- Organic Food and Agriculture
- Animal Nutrition and Physiology
- Hemoglobin structure and function
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Growth Hormone and Insulin-like Growth Factors
- Cardiovascular Function and Risk Factors
- Occupational exposure and asthma
- Lysosomal Storage Disorders Research
- Nitric Oxide and Endothelin Effects
- Environmental Toxicology and Ecotoxicology
- Cardiac Arrest and Resuscitation
- Glycogen Storage Diseases and Myoclonus
St. Antonius Ziekenhuis
2023-2024
University Medical Center Utrecht
2023-2024
University of Twente
2023
Radboud University Nijmegen
2005
Radboud University Medical Center
2005
Universität Hamburg
1989-2001
University Medical Center Hamburg-Eppendorf
1997
Hamburg Institut (Germany)
1997
Microinjection of human Jurkat T-lymphocytes with nicotinic acid adenine dinucleotide phosphate (NAADP+) dose-dependently stimulated intracellular Ca2+-signaling. At a concentration 10 nM NAADP+ evoked repetitive and long-lasting Ca2+-oscillations low amplitude, whereas at 50 100 nM, rapid high initial Ca2+-peak followed by trains smaller was observed. Higher concentrations (1 μM) gradually reduced the Ca2+-peak, complete self-inactivation Ca2+-signals seen μM. The effect specific as it not...
Cyclic ADP-ribose (cADPr) is a potent Ca2+-mobilizing natural compound (Lee, H. C., Walseth, T. F., Bratt, G. T., Hayes, R. N., and Clapper, D. L. (1989) J. Biol. Chem. 264, 1608-1615) which has been shown to release Ca2+ from an intracellular store of permeabilized T-lymphocytes (Guse, A. H., Silva, C. P., Emmrich, Ashamu, G., Potter, B. V. L., Mayr, W. (1995) Immunol. 155, 3353-3359). Microinjection cADPr into intact single T lymphocytes dose dependently induced repetitive but irregular...
Cultured cardiac myocytes from adult Sprague-Dawley rats express both insulin-like growth factor-I (IGF-I) receptors and factor-II/mannose 6-phosphate (IGF-II/Man6P) respond to IGF-I with a dose-dependent accumulation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] 1,4-bisphosphate [Ins(1,4)P2]. Specific binding [125I]IGF-I isolated membranes cultured amounted 1-1.2%. Binding was inhibited by unlabeled at nanomolar concentrations insulin much higher concentrations. These data suggest that...
Manual contouring of neurovascular structures on prostate magnetic resonance imaging (MRI) is labor-intensive and prone to considerable interrater disagreement. Our aim contour automatically MRI by deep learning (DL) improve workflow agreement.Segmentation was performed pre-treatment 3.0 T data 131 cancer patients (training [n = 105] testing 26]). The include the penile bulb (PB), corpora cavernosa (CCs), internal pudendal arteries (IPAs), bundles (NVBs). Two DL networks, nnU-Net DeepMedic,...
The release of reactive oxygen intermediates (ROI) from bovine alveolar macrophages (BAM) after stimulation with heavy metal-containing dusts was investigated. BAM were obtained by postmortem lavages lungs. collected waste incineration, sewage sludge an electric power station, and two different factories. Three quartz used as metal-free controls. fractionated sieving sedimentation analyzed electron microscopy, atomic absorption spectrometry (AAS), emission inductively coupled plasma...
Previous studies demonstrated the accumulation of inositol mono- and poly-phosphates in carbamoylcholine-stimulated cultured cardiac ventricular myocytes adult rats [Berg, Guse & Gercken (1989) Biochim. Biophys. Acta 1010, 100-107]. Stimulation with noradrenaline (50 microM) presence propranolol (10 led to a time-dependent pattern cardiac-ventricular myocytes. Ins(1,4,5)P3 Ins(1,3,4,5)P4 increased rapid initial phase. The degradation products Ins(1,4,5)P3, namely Ins(1,4)P2 Ins(4)P,...
Adequate detection of the histopathological extraprostatic extension (EPE) prostate cancer (PCa) remains a challenge using conventional radiomics on 3 Tesla multiparametric magnetic resonance imaging (3T mpMRI). This study focuses assessment artificial intelligence (AI)-driven models with innovative MRI in predicting EPE at lesion-specific level. With dataset encompassing 994 lesions from 794 PCa patients who underwent robot-assisted radical prostatectomy (RARP) two Dutch hospitals,...
Abstract Background Pelvic morphological parameters on magnetic resonance imaging (MRI), such as the membranous urethral length (MUL), can predict urinary incontinence after radical prostatectomy but are prone to interobserver disagreement. Our objective was improve agreement among radiologists in measuring pelvic using deep learning (DL)-based segmentation of structures MRI scans. Methods Preoperative collected from 167 prostate cancer patients undergoing within our regional multicentric...
Recently, we provided evidence for cyclic adenosine 5′‐diphosphate‐ribose, cADP‐ribose, as a second messenger in Jurkat T‐lymphocytes upon stimulation of the T‐cell receptor/CD3‐ complex ( Guse et al ., 1999 ). cADP‐ribose mobilizes Ca 2+ from an intracellular store which is sensitive to caffeine and gated by ryanodine receptor/Ca release channel. In present study investigated ability trypanocidal drug, suramin, activate receptor T‐cells. Since suramin cannot permeate plasma membrane, it was...
The mineral‐dust induced activation of pulmonary phagocytes is thought to be involved in the induction severe lung diseases. bovine alveolar macrophages (BAM) by silica was investigated flow cytometry. Short‐term incubation (10 min) BAM with gel and quartz dust particles increases cytosolic free calcium concentration ([Ca 2+ ] i ), decreases intracellular pH (pH plasma membrane potential (PMP). extent these changes dependent, related type due Ca influx from extracellular medium. An increase...
Inhalable particulate dusts are involved in the genesis of several lung diseases. Besides well-known toxic dusts, i.e., asbestos and quartz, heavy metal-containing pollutants considered as possible harmful substances. In present study, we compared effect silica chemically coated with certain metal oxides from industrial productions on cell physiological parameters bovine alveolar macrophages (BAM).The cytosolic free calcium concentration, [Ca2+](i), intracellular pH (pH(i)), plasma membrane...
We appreciate the comments made by Grivas et al regarding our article on automated contouring of neurovascular structures prostate MRI [[1]van den Berg I. Savenije M.H.F. Teunissen F.R. van de Pol S.M.G. Rasing M.J.A. Melick H.H.E. al.Deep learning for magnetic resonance imaging cancer patients.Phys Imaging Radiat Oncol. 2023; 26100453https://doi.org/10.1016/j.phro.2023.100453Abstract Full Text PDF Scopus (1) Google Scholar]. are thankful recognizing potential artificial intelligence model...
Inhalable particulate dusts are involved in the genesis of several lung diseases. Besides well-known toxic dusts, i.e. asbestos and quartz, heavy metal-containing pollutants considered as possible harmful substances. In present study we compared effect silica chemically coated with certain metal oxides on cell physiological parameters bovine alveolar macrophages (BAM). The cytosolic free calcium concentration [(Ca<SUP>2+</SUP>)<SUB>i</SUB>], intracellular pH (pH<SUB>i</SUB>), plasma membrane...