A5051145011- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antimicrobial Peptides and Activities
- Immune cells in cancer
- Veterinary Equine Medical Research
- Immunotherapy and Immune Responses
- Advanced Proteomics Techniques and Applications
- Nitric Oxide and Endothelin Effects
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- S100 Proteins and Annexins
- Veterinary Orthopedics and Neurology
- Cytomegalovirus and herpesvirus research
- IL-33, ST2, and ILC Pathways
- Medicinal Plant Pharmacodynamics Research
- Eosinophilic Esophagitis
- Nanoparticle-Based Drug Delivery
- Neuropeptides and Animal Physiology
- Monoclonal and Polyclonal Antibodies Research
- Psoriasis: Treatment and Pathogenesis
- Mycobacterium research and diagnosis
- Poverty, Education, and Child Welfare
- Neuroscience and Neuropharmacology Research
- Cytokine Signaling Pathways and Interactions
- Receptor Mechanisms and Signaling
NIHR Newcastle Biomedical Research Centre
2024-2025
Newcastle University
2020-2025
Centre for Inflammation Research
2019-2025
University of Edinburgh
2019-2025
University of Newcastle Australia
2024
Immune Regulation (United Kingdom)
2024
MRC Centre for Regenerative Medicine
2024
The Ohio State University
2006-2012
University of Cambridge
2012
Guelph General Hospital
2012
Abstract The host defence peptide cathelicidin (LL-37 in humans, mCRAMP mice) is released from neutrophils by de-granulation, NETosis and necrotic death; it has potent anti-pathogen activity as well being a broad immunomodulator. Here we report that powerful Th17 potentiator which enhances aryl hydrocarbon receptor (AHR) RORγt expression, TGF-β1-dependent manner. In the presence of TGF-β1, enhanced SMAD2/3 STAT3 phosphorylation, profoundly suppressed IL-2 T-bet, directing T cells away Th1...
Neutrophils and T cells exist in close proximity lymph nodes inflamed tissues during health disease. They are able to form stable interactions, with profound effects on the phenotype function of cells. However, outcome these frequently contradictory; some systems neutrophils suppress cell proliferation, others they activatory or present antigen directly. Published protocols modelling interactions vitro do not reflect full range found vivo ; examine how activated naïve differentially respond...
Efficient and site-specific delivery of therapeutics drugs remains a critical challenge in cancer treatment. Traditional drug nanocarriers such as antibody-drug conjugates are not generally accessible due to their high cost can lead serious side effects including life-threatening allergic reactions. Here, these problems overcome via the engineering supramolecular agents that manufactured with an innovative double imprinting approach. The developed molecularly imprinted nanoparticles...
Multiple Sclerosis (MS) is a complex auto-inflammatory disease affecting the brain and spinal cord, which results in axonal de-myelination symptoms including fatigue, pain, difficulties with vision mobility. The involvement of immune system pathology MS well established, particularly adaptive T cell response, there has been particular focus on IL-17-producing subset Th17 cells their role driving disease. However, importance innate not so characterised. Here we focused neutrophils, are rapid...
ABSTRACT Regulatory T cells (Tregs) are important in maintaining tolerance and key players immunity. In aging, increased Treg function along with low‐grade inflammation has been reported. This dichotomy of enhanced highlights the importance understanding biology communication patterns very old. this proof‐of‐concept study, we demonstrate that aged Tregs (85 years) do not significantly communicate CD4 + CD8 effectors when compared healthy < 66‐year‐olds. Of note was CD3 CD56 CD161 −...
Multiple sclerosis (MS) is a highly prevalent demyelinating autoimmune condition; the mechanisms regulating its severity and progression are unclear. The IL-17-producing Th17 subset of T cells has been widely implicated in MS mouse model, experimental encephalomyelitis (EAE). However, differentiation regulation during EAE remain incompletely understood. Although evidence mounting that antimicrobial peptide cathelicidin profoundly affects early cell differentiation, no studies have looked at...
Abstract Objective —To identify patterns and correlations of gross, histologic, gene expression characteristics articular cartilage from horses with osteoarthritis. Animals —10 clinically normal 11 osteoarthritis the metacarpal condyles. Procedures —Metacarpophalangeal joints were opened digitally photographed, gross lesions scored quantified. Representative specimens stained for histologic scoring. Total RNA dorsal palmar surfaces was processed on an equine microarray. Results —Histologic...
Summary Antimicrobial host defence peptides (HDP) are critical for the first line of against bacterial, viral, and fungal pathogens. Over past decade we have become more aware that, in addition to their antimicrobial roles, they also possess potent immunomodulatory capacity. This includes chemoattracting immune cells, activating dendritic cells macrophages, altering T-cell differentiation. Most examinations roles focused on tissues which very abundant, such as intestine inflamed skin....
Abstract Neuronal nitric oxide synthase (nNOS) catalyses the production of neurotransmitter oxide. nNOS is expressed in dorsal raphe nucleus (DRN), a source ascending serotonergic projections. In this study, we examined distribution and function DRN adjacent median (MRN) rat. We hypothesized that differentially across nuclei influences firing activity subgroup 5‐HT neurons. Immunohistochemistry revealed that, present around 40% neurons, throughout MRN, as well some non‐5‐HT neurons...
A 4-month-old Standardbred colt was examined because of a fractured right calcaneus 8 days' duration with increased distraction the fracture fragment evident on sequential radiographs.The foal severely lame diffuse periarticular tarsal swelling. Radiographically, complete, displaced long oblique calcaneal body evident. Because gap increasing time and lameness remained severe, despite medical management, surgical repair recommended.The anesthetized, minimally invasive reduction internal...
Abstract Multiple Sclerosis (MS) is a complex auto-inflammatory disease affecting the brain and spinal cord, which results in axonal de-myelination symptoms including fatigue, pain, difficulties with vision mobility. The involvement of immune system pathology MS well established, particularly adaptive T cell response, there has been particular focus on IL-17-producing subset Th17 cells their role driving disease. However, importance innate not so characterised. Here we focused neutrophils,...
ABSTRACT The host defence peptide cathelicidin (LL-37 in humans, mCRAMP mice) is released from neutrophils by de-granulation, NETosis and necrotic cell death; it has potent antibacterial, antiviral antifungal activity as well being a powerful immunomodulator. It proximity to CD4 + T cells during inflammatory infectious disease but its impact on phenotype scarcely understood. Here we demonstrate that Th17 potentiating factor which increases expression of the aryl hydrocarbon receptor (AHR)...
Abstract Innate lymphoid cells (ILCs) are tissue-resident immune that have been recently implicated in initiating and driving anti-tumor responses. ILCs classified into three main groups, namely type 1 (ILC1), 2 ILCs, 3 ILCs. All groups either eliciting pro or responses different cancer subtypes with the consensus cannot be overlooked within field of In this review, we will specifically expand on knowledge ILC1, their characterization, function, plasticity anti-cancer Within premise, discuss...
Abstract Multiple Sclerosis (MS) is a highly prevalent demyelinating autoimmune condition; the mechanisms regulating its severity and progression are unclear. The IL-17-producing Th17 subset of T cells has been widely implicated in MS mouse model, experimental encephalomyelitis (EAE). However, differentiation regulation during EAE remain incompletely understood. Although evidence mounting that antimicrobial peptide cathelicidin profoundly affects early cell differentiation, no studies have...
Abstract Neutrophils and T cells exist in close proximity lymph nodes inflamed tissues during health disease. They are able to form stable interactions, with profound effects on the phenotype function of cells. However, outcome these frequently contradictory; some systems neutrophils suppress cell proliferation, others they activatory or present antigen directly. Published protocols modelling interactions vitro do not reflect full range found vivo ; examine how activated naïve differentially...
Analysis of immunofluorescence is commonly confounded by autofluorescence and non-specific staining in the colorectum, particularly within highly inflamed samples. There no consensus on how to definitively prevent this, study protocols are variable. Our laboratory identified strong negative control samples stained for numerous target antigens, including CD3. Following methodical attempts ensure there was reagent contamination, we concluded that this autofluorescence/ staining. Significant...