A5001834482- Fungal Plant Pathogen Control
- Plant Pathogens and Fungal Diseases
- Mycotoxins in Agriculture and Food
- Plant-Microbe Interactions and Immunity
- Agriculture, Plant Science, Crop Management
- Plant Virus Research Studies
- Mycorrhizal Fungi and Plant Interactions
- Plant Disease Resistance and Genetics
- Yeasts and Rust Fungi Studies
- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Plant and fungal interactions
- Plant and Fungal Interactions Research
- Postharvest Quality and Shelf Life Management
- Plant Pathogens and Resistance
- Plant Pathogenic Bacteria Studies
- Microbial Natural Products and Biosynthesis
- Garlic and Onion Studies
- Forest Insect Ecology and Management
- Fungal Biology and Applications
- Insect and Pesticide Research
- Nematode management and characterization studies
- Plant pathogens and resistance mechanisms
- Botanical Research and Chemistry
- Fungal and yeast genetics research
Tottori University
2012-2024
Kyoto University
2013
Plant (United States)
2013
Yamagata University
2012
Fracture Analysis Consultants (United States)
2011
Food Research Institute
2011
Forestry and Forest Products Research Institute
2011
Planta
2009
Niigata University
2006
Tokushima Bunri University
2004
The Solanaceae family includes several economically important vegetable crops. tomato (Solanum lycopersicum) is regarded as a model plant of the family. Recently, number resources have been developed in parallel with ongoing genome sequencing project. In particular, miniature cultivar, Micro-Tom, system genomics, and genomics Micro-Tom-background, such ESTs mutagenized lines, established by an international alliance. To accelerate progress we collection fully-sequenced 13,227 Micro-Tom...
The tomato pathotype of Alternaria alternata produces host-specific AAL toxin and causes stem canker on tomato. A polyketide synthetase (PKS) gene, ALT1, which is involved in biosynthesis, resides a 1.0-Mb conditionally dispensable chromosome (CDC) found only the pathogenic toxin-producing strains. Genomic sequences ALT1 another PKS both reside CDC strains, were compared to those strains collected worldwide. This revealed that genes identical among five A. having different geographical...
Alternaria alternata apple pathotype causes blotch of susceptible cultivars through the production a cyclic peptide host-specific toxin, AM-toxin. PCR (polymerase chain reaction), with primers designed to conserved domains synthetase genes, amplified several products from A. that showed high similarity other fungal synthetases and were specific pathotype. Screening Lambda Zap genomic library these PCR-generated probes identified overlapping clones containing complete gene 13.1 kb in length...
There are now nine or more Alternaria pathogens that produce host-specific toxins, and the structures of most toxins have been elucidated. classified in three groups terms primary site action. ACT-, AF-, AK-toxins common an epoxy-decatrienoic acid structure exert their effect on plasma membrane susceptible cells. A rapid increase electrolyte loss from tissues invaginations membranes effects these toxins. The second group is represented by ACR(L)-toxin, which induces changes mitochondria,...
Host-specific toxins are produced by three pathotypes of Alternaria alternata: AM-toxin, which affects apple; AK-toxin, Japanese pear; and AAL-toxin, tomato. Each toxin has a role in pathogenesis. To facilitate molecular genetic analysis production, isolation toxin-deficient mutants utilizing ectopic integration plasmid DNA been attempted. However, the transformation frequency was low, events most transformants were complicated. Addition restriction enzyme during reported to increase...
Tomato has been a good model plant to analyze plant-pathogen interactions and its prospects for the future are promising. An international consortium named International Solanaceae Genomics Project (SOL) is proceeding with whole genome sequencing of tomato. In order be relevant in post-genomic era, accumulation information on tomato-pathogen important. this review, following topics addressed from perspective pathology: cultivars tomato, wild species disease-resistance modern breeding fungal,...
Heterologous expression of four clade-A bifunctional terpene synthases (BFTSs), giving di/sesterterpenes with unique polycyclic carbon skeletons such as sesterfisherol, enabled the isolation sesterterpenes Bm1, Bm2, Bm3, and Pb1. Their structures suggested that formation products occurs via various diastereomeric carbocation intermediates, allowing proposal BFTSs catalyze three-step cyclizations using several stereofacial combinations allylic cation–olefin pairs in corresponding...
To investigate plant programmed cell death (PCD), we developed the model system using phytotoxin AAL, which is produced by necrotrophic pathogen Alternaria alternata f. sp. lycopersici, and AAL-sensitive Nicotiana umbratica. We previously reported that ethylene (ET) signaling plays a pivotal role in AAL-triggered (ACD). However, downstream of ET to ACD remains unclear. Here, show modulator AAL 1 (MACD1), an APETALA2/ET response factor (ERF) transcription factor, participates acts during ACD....
Heterologous expression of four candidate genes found in ophiobolin gene clusters from three fungal strains was employed to elucidate the late-stage biosynthetic pathway phytotoxin ophiobolin. Expression oblBAc (cytochrome P450) cryptic cluster gave unexpected products, and that oblBBm/oblBEv producers, with oblDBm as transporter, yielded intermediate C through an unusual four-step oxidation process. The observation made this study may provide a useful guideline for elucidation genuine...
The apple pathotype of Alternaria alternata produces host-specific AM-toxin and causes blotch apple. Previously, we cloned two genes, AMT1 AMT2, required for biosynthesis found that these genes are encoded by small, supernumerary chromosomes <1.8 Mb in the strains. Here, performed expressed sequence tag analysis 1.4-Mb chromosome encoding AMT strain IFO8984. A cDNA library was constructed using RNA from AM-toxin–producing cultures. total 40,980 clones were screened with probe, 196...
Fusarium oxysporum is an ascomycetous fungus that well-known as a soilborne plant pathogen. In addition, large population of nonpathogenic F. (NPF) inhabits various environmental niches, including the phytosphere. To obtain insight into origin pathogenic oxysporum, we focused on tomato (Solanum lycopersicum) and its f. sp. lycopersici (FOL). We collected from wild transition Solanum spp. modern cultivars in Chile, Ecuador, Peru, Mexico, Afghanistan, Italy, Japan, evaluated fungal isolates...
Alternaria alternata apple pathotype (previously A. mali) causes blotch on susceptible cultivars through the production of a host-specific toxin, AM-toxin. Identification some species, especially those that produce toxins, has been extremely difficult due to high level variability which extends even nonpathogenic isolates. We have recently cloned and characterized gene (AMT) plays crucial role in AM-toxin biosynthesis demonstrated it is only present isolates pathotype. Using primers designed...