A5070868391- Reconstructive Surgery and Microvascular Techniques
- Reconstructive Facial Surgery Techniques
- Lymphatic System and Diseases
- Breast Implant and Reconstruction
- Body Contouring and Surgery
- Bone fractures and treatments
- Mesenchymal stem cell research
- Diagnosis and Treatment of Venous Diseases
- Tracheal and airway disorders
- Surgical site infection prevention
- Trauma Management and Diagnosis
- Orthopedic Surgery and Rehabilitation
- Nerve Injury and Rehabilitation
- Breast Cancer Treatment Studies
- Tissue Engineering and Regenerative Medicine
- Cancer, Hypoxia, and Metabolism
- Head and Neck Cancer Studies
- Organ and Tissue Transplantation Research
- Wound Healing and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Systemic Sclerosis and Related Diseases
- Surgical Sutures and Adhesives
- Digital Imaging in Medicine
- Anatomy and Medical Technology
- Cleft Lip and Palate Research
The University of Texas MD Anderson Cancer Center
2016-2025
Northwestern University
2023-2024
University of Wisconsin–Madison
2023
Creative Commons
2016-2023
University of Southern California
2023
Methodist Hospital
2016-2023
The University of Texas Health Science Center at Houston
2023
Indiana University – Purdue University Indianapolis
2023
Madigan Army Medical Center
2023
Instituto Federal de Educação, Ciência e Tecnologia de Minas Gerais
2023
Hypertrophic scars occur following cutaneous wounding and result in severe functional esthetic defects. The pathophysiology of this process remains unknown. Here, we demonstrate for the first time that mechanical stress applied to a healing wound is sufficient produce hypertrophic mice. resulting are histopathologically identical human persist more than six months brief (one-week) period augmented during proliferative phase healing. Resulting structurally showed dramatic increases volume...
Diabetes is associated with poor outcomes following acute vascular occlusive events. This results in part from a failure to form adequate compensatory microvasculature response ischemia. Since endothelial growth factor (VEGF) an essential mediator of neovascularization, we examined whether hypoxic up-regulation VEGF was impaired diabetes. Both fibroblasts isolated type 2 diabetic patients, and normal exposed chronically high glucose, were defective their capacity up-regulate hypoxia. In...
Background— Advanced age is known to impair neovascularization. Because endothelial progenitor cells (EPCs) participate in this process, we examined the effects of aging on EPC recruitment and vascular incorporation. Methods Results— Murine neovascularization was by use an ischemic flap model, which demonstrated aged mice (19 24 months) had decreased mobilization (percent mobilized 1.4±0.2% versus 0.4±0.1%, P <0.005) that resulted impaired gross tissue survival compared with young (2 6...
AbstractDiabetic wounds are a significant public health burden, with slow or non-healing diabetic foot ulcers representing the leading cause of non-traumatic lower limb amputation in developed countries. These heal poorly as result compromised blood vessel formation response to ischemia. We have recently shown that this impairment neovascularization results from high glucose-induced defect transactivation hypoxia-inducible factor-1α (HIF-1α), transcription factor regulating vascular...
Pulsed electromagnetic fields (PEMF) have been shown to be clinically beneficial, but their mechanism of action remains unclear. The present study examined the impact PEMF on angiogenesis, a process critical for successful healing various tissues. increased degree endothelial cell tubulization (sevenfold) and proliferation (threefold) in vitro. Media from cultures had similar stimulatory effect, heat denaturation ablated this activity. In addition, conditioned media was able induce...
Background: Chronic wounds, particularly in diabetics, result significant morbidity and mortality have a profound economic impact. The authors demonstrate that pulsed electromagnetic fields significantly improve both diabetic normal wound healing 66 mice through up-regulation of fibroblast growth factor (FGF)-2 are able to prevent tissue necrosis after an ischemic insult. Methods: Db/db C57BL6 were wounded exposed fields. Gross closure, cell proliferation, vascularity assessed. Cultured...
Background The free vascularized omental lymphatic flap provides an option without the risk for iatrogenic donor site lymphedema that plagues alternative lymph node transfer sites. has been associated with significant morbidity in past; however, modern techniques and advanced technology, a minimally invasive approach to harvest is feasible. We present long‐term outcomes of treatment lymphedema. Methods All consecutive patients undergoing were included. Perioperative evaluation included...
Background: This study aimed to provide a comprehensive analysis of factors that might contribute abdominal donor-site morbidity after abdominally based free flap breast reconstruction. Methods: The authors performed retrospective all reconstructions from January 2000 through December 2010 at their institution. Results: Overall, 89 1507 patients developed an bulge/hernia (unilateral: 57 1044; bilateral: 32 463). A unilateral transverse rectus abdominis musculocutaneous (TRAM) was...
National and international experts in inflammatory breast cancer (IBC) from high-volume centers treating IBC recently convened at the 10th Anniversary Conference of Morgan Welch Inflammatory Breast Cancer Research Program The University Texas MD Anderson Center Houston Texas. A consensus on clinical management patients with was discussed, summarized, subsequently reviewed. All participants conference (patients, advocates, researchers, trainees, clinicians) were queried using MDRing...
Risk factors and techniques for free flap salvage in head neck reconstruction are poorly described.We conducted a retrospective review of all flaps performed from 2000 to 2010.Overall, 151 2296 (6.6%) underwent microvascular complications. Age, comorbidities, surgeon experience (p = .88), vein grafts, supercharging .45) did not affect salvage. Muscle-only .002) were associated with significantly worse outcomes. Coupled venous anastomoses superior handsewn .03). Arteriovenous thrombosis had...
Evolving evidence suggests a possible role for adipose stromal cells (ASCs) in adult neovascularization, although the specific cues that stimulate their angiogenic behavior are poorly understood. We evaluated effect of hypoxia, central mediator new blood vessel development within ischemic tissue, on proneovascular ASC functions. Murine ASCs were exposed to normoxia (21% oxygen) or hypoxia (5%, 1% varying lengths time. Vascular endothelial growth factor (VEGF) secretion by increased as an...
The PEAK PlasmaBlade is a new electrosurgical device that uses pulsed radiofrequency to generate plasma-mediated discharge along the exposed rim of an insulated blade, creating effective cutting edge while blade stays near body temperature.Full-thickness incisions were made on dorsums pigs with PlasmaBlade, conventional device, and scalpel, blood loss was quantified. Wounds harvested at designated time points, tested for wound tensile strength, examined histologically scar formation tissue...
Aging and diabetes are major risk factors for poor wound healing tissue regeneration that reflect an impaired ability to respond ischemic insults. The authors explored the intrinsic neovascular potential of adipose-derived stromal cells in setting advanced age type 1 2 diabetes.Adipose-derived isolated from young, aged, streptozotocin-induced, db/db diabetic mice were exposed normoxia hypoxia vitro. Vascular endothelial growth factor (VEGF) expression, proliferation, tubulization measured....
Background: In designing an osteocutaneous fibula flap, poor planning, aberrant anatomy, or inadequate perforators may necessitate modification of the flap design, exploration contralateral leg, additional harvest. The authors studied predictive power computed tomographic angiography in planning and execution. Methods: a prospective cohort 40 consecutive patients who underwent preoperative mapping peroneal artery its subsequent free reconstruction mandibular maxillary defects. compared their...
Designing a reliable fibula flap skin paddle can be challenging because of the lack information on precise perforator locations. The purpose this study was to precisely map perforators and provide simple method for design.Eighty consecutive patients undergoing free reconstruction were included in prospectively designed study. location, size, type recorded intraoperatively mapped line connecting fibular head lateral malleolus.There 46 male 34 female with total 202 perforators. average length...
Background: Despite advances in surgical training, microsurgery is still based on an apprenticeship model. To evaluate skill acquisition and apply targeted feedback to improve their training model, the authors applied Structured Assessment of Microsurgery Skills microsurgical fellows. They hypothesized that subjects would demonstrate measurable improvement performance throughout study period consistently across evaluators. Methods: Seven fellows were evaluated during 118 cases by 16...
Loss of a breast free flap is relatively rare but catastrophic occurrence. Our study aims to identify risk factors for loss and assess whether different salvage techniques affect salvage. We performed retrospective review all flaps at single institution from 2000 2010. Overall, 2138 were in 1608 patients (unilateral, 1120 bilateral, 488) with 44 losses (2.1%). Age, body mass index, smoking, radiation, chemotherapy, surgeon experience did not loss. Abdominal based on perforator significantly...
Background: A tracheal matrix scaffold decellularized by detergent-enzymatic treatment has been shown as a promising in tissue engineering. The objectives of this study were to evaluate the impact technique on extracellular integrity and characterize environment for recellularization. Methods: Brown Norway rat tracheae using modified treatment. Antigenicity cellularity monitored during processing. Glycosaminoglycan content, histoarchitecture, mechanical properties also evaluated. Matrix...
Many surgeons are hesitant to use interposition vein grafting in head and neck microvascular free flap surgery because of concerns for elevated risk loss.The authors conducted a review patients who underwent reconstruction between 2005 2015. The effect grafts on compromise loss was analyzed using univariate multivariate models.A total 3240 flaps were performed. Vein used 241 (7.4 percent). rate 14.5 percent with 3.4 without (p < 0.001). 6.4 1.1 Radiation therapy, chemotherapy, prior...