A5037482684- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Genomics and Phylogenetic Studies
- Polysaccharides and Plant Cell Walls
- Plant Genetic and Mutation Studies
- Ubiquitin and proteasome pathways
- Plant tissue culture and regeneration
- Lipid metabolism and biosynthesis
- Plant-Microbe Interactions and Immunity
- Mitochondrial Function and Pathology
Southwest University of Science and Technology
2023-2024
Rice Research Institute
2016-2024
Southwest University
2016-2023
Pollen development is critical to plant reproduction, but the underlying regulatory molecular mechanisms have not been fully elucidated. The Arabidopsis (Arabidopsis thaliana) EFR3 OF PLANT 3 (EFOP3) and 4 (EFOP4) genes encode members of Armadillo (ARM) repeat superfamily that play key roles in pollen development. Herein, we demonstrate EFOP3 EFOP4 are co-expressed at anther stages 10-12, loss-of-function both leads male gametophyte sterility, irregular intine, shriveled grains stage 12. We...
Zebra leaf mutants are an important resource for studying colour in rice. In most such mutants, the zebra phenotype results from defective chloroplast biogenesis. The molecular mechanism by which leaves develop remains unclear, so additional zebra-leaf need to be identified.We isolated a novel rice mutant, named 15 (z15), showed transversely striped with yellow-green or white-green sectors, structure was disturbed. Transmission electron microscopy revealed that of various organelles impaired...
The enzyme C-14 sterol reductase is involved in biosynthesis of brassinosteroids (BR) and sterols, as well plant development. OsFK1, a member the pathway located endoplasmic reticulum (ER), encodes reductase. However, there little research on function rice. Compared with wild type, an osfk1 mutant showed dwarf phenotype premature aging second leaf during trefoil stage, abnormal development veins tillering stage. signs aberrant PCD, evidenced by TUNEL staining. This suggested that high ROS...
Galacturonosyltransferase (GalAT) is required for the synthesis of pectin, an important component plant cell walls that also involved in signal transduction. Here, we describe rice (Oryza sativa) male-sterile mutant O. sativa pectin-defective tapetum1 (ospdt1), which GalAT mutated. The ospdt1 exhibited premature programmed death (PCD) tapetum and disordered pollen walls, resulting aborted grains. Pectin distribution anther sac was comparable between wild-type, suggesting structural pectin...
Abstract Background Mitochondria are vital regulators of plant growth and development, constitute the predominant source ATP, participate in multiple anabolic catabolic metabolic pathways. But mechanism by which dysfunctional mitochondria affect remains unknown, more mitochondria-defective mutants need to be identified. Results A mutant decreased vascular bundle 1 ( dvb1 ) was isolated from rice library mutagenized EMS (ethylmethane sulfonate), shows dwarfism, narrow leaves, short branches,...
As an essential process in the life of a plant, natural leaf senescence is necessary for adaptation to environmental diversity, whereas early can reduce per‐unit yield and cause inferior harvest quality during crop production. To explore molecular mechanism rice ( Oryza sativa L.), we studied esl11 ) mutant obtained by ethyl methane sulfonate (EMS) mutagenesis. The leaves showed normal growth coloration prior three‐leaf stage, but after this all fully expanded underwent yellowing from edges...
The mitogen-activated protein kinase (MAPK) cascade is important in stress signal transduction and plant development. In the present study, we identified a rice (Oryza sativa L.) mutant with reduced fertility, Oryza 6 (osmapk6), which harbored mutated MAPK gene. Scanning transmission electron microscopy, quantitative RT-PCR analysis, TUNEL assays, RNA situ hybridization, longitudinal transverse histological sectioning, map-based cloning were performed to characterize osmapk6 mutant. gene...
In the past decade, exploration of genetic resources in rice has significantly enhanced efficacy breeding. However, is hindered by identification candidate genes. To expedite genes, this study examined tapetum programmed cell death-related genes OsiWAK1, OsPDT1, EAT1, TDR, and TIP2 to assess Dual-Luciferase (Dual-LUC) assay rapidly determining gene relationships. The found that, Dual-LUC assay, OsiWAK1 its various recombinant proteins exhibit comparable activation abilities on EAT1 promoter,...
Rice lesion mimic mutants are important materials for studying the mechanisms of cell death. In-depth research on these can provide insights into molecular underlying rice growth and development, offering a theoretical basis crop improvement. In this study, variety Wuyunjing 21 (WYJ21) was mutagenized with ethyl methanesulfonate to obtain mutant, lmm28. Unlike wild-type (WT) plants, lmm28 mutant exhibits brown lesions leaves starting from early tillering stage. The size increases as plant...
This study was conducted on the lesion-mimic mutant lm5, which produced by mutagenesis of WYJ21 (WT) using ethyl methane sulfonate (EMS). The lm5 short in seedling stage and displayed yellowish-brown disease-like spots leaves that were when plant at tillering stage. gradually grew larger as until it reached maturity. Compared to WT, had considerably reduced height, ear panicle length, tiller number, 1000-grain weight. A single recessive gene found be control according a genetic study. It...