A5015827942- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Helicobacter pylori-related gastroenterology studies
- Legume Nitrogen Fixing Symbiosis
- Amino Acid Enzymes and Metabolism
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- RNA modifications and cancer
- Galectins and Cancer Biology
- Plant nutrient uptake and metabolism
- Microbial Metabolites in Food Biotechnology
- Genomics and Phylogenetic Studies
- Escherichia coli research studies
- Metalloenzymes and iron-sulfur proteins
- Protist diversity and phylogeny
- Polyamine Metabolism and Applications
- Veterinary medicine and infectious diseases
- Wastewater Treatment and Nitrogen Removal
- Salmonella and Campylobacter epidemiology
- Plant Micronutrient Interactions and Effects
- Glycosylation and Glycoproteins Research
- Microbial Community Ecology and Physiology
- Pediatric Hepatobiliary Diseases and Treatments
- Caveolin-1 and cellular processes
- Vibrio bacteria research studies
University of Georgia
2014-2025
Centers for Disease Control and Prevention
2013
Cornell University
2002
Emory University
2002
National Institutes of Health
1994
University of California, Berkeley
1989-1992
University of Wisconsin–Madison
1986-1989
ABSTRACT The 6.10-Mb genome sequence of the aerobic chitin-digesting gliding bacterium Flavobacterium johnsoniae (phylum Bacteroidetes ) is presented. F. a model organism for studies bacteroidete motility, gene regulation, and biochemistry. mechanism novel, analysis confirms that it does not involve well-studied motility organelles, such as flagella or type IV pili. machinery composed Gld proteins in cell envelope are thought to comprise “motor” SprB, which function surface adhesin propelled...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHomocitrate is a component of the iron-molybdenum cofactor nitrogenaseTimothy R. Hoover, Juan Imperial, Paul W. Ludden, and Vinod K. ShahCite this: Biochemistry 1989, 28, 7, 2768–2771Publication Date (Print):April 4, 1989Publication History Published online1 May 2002Published inissue 4 April 1989https://pubs.acs.org/doi/10.1021/bi00433a004https://doi.org/10.1021/bi00433a004research-articleACS PublicationsRequest reuse permissionsArticle...
Summary Co‐ordinated regulation of gene expression is required for the transmission and survival Borrelia burgdorferi in different hosts. The sigma factor RpoS (σ S ), as regulated by RpoN 54 has been shown to regulate key virulence factors (e.g. OspC) these processes. As important, multiple signals temperature, pH, cell density, oxygen) have increase σ ‐dependent genes; however, little known about signal transduction mechanisms that modulate rpoS . In this report we show that: (i) a...
In two-component signal transduction, an input triggers phosphorylation of receiver domains that regulate the status output modules. One such module is AAA+ ATPase domain in bacterial enhancer-binding proteins remodel σ 54 form RNA polymerase. We report X-ray solution scattering and electron microscopy structures activated, full-length nitrogen-regulatory protein C (NtrC) showing a novel mechanism for regulation assembly via juxtaposition ring. Accompanying hydrolysis cycle required...
The helical shape of the human stomach pathogen Helicobacter pylori has been suggested to provide mechanical advantage for penetrating viscous mucus layer. Using single-cell tracking and quantitative morphology analysis, we document marked variation in cell body parameters flagellum number among H. strains leading distinct broad speed distributions broth gastric mucin media. These reflect both temporal swimming morphologic within population. Isogenic mutants with straight-rod showed 7-21%...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSubstrate reduction properties of dinitrogenase activated in vitro are dependent upon the presence homocitrate or its analogs during iron-molybdenum cofactor synthesisJuan Imperial, Timothy R. Hoover, Mark S. Madden, Paul W. Ludden, and Vinod K. ShahCite this: Biochemistry 1989, 28, 19, 7796–7799Publication Date (Print):September 1989Publication History Published online1 May 2002Published inissue 19 September...
Helicobacter pylori is a Gram-negative bacterium and human pathogen that linked to various gastric diseases, including peptic ulcer disease, chronic gastritis, cancer. The filament of the H. flagellum surrounded by membranous sheath contiguous with outer membrane. Proteomic analysis isolated sheathed flagella from B128 identified lipoprotein HP0135 as potential component flagellar sheath. small protein, mature only 28 amino acid residues in length. Deletion hp0135 resulted morphological...
The Helicobacter pylori flagellar motor contains several accessory structures that are not found in the archetypal Escherichia coli and Salmonella enterica motors. H . hp0838 encodes a previously uncharacterized lipoprotein is an operon with flgP , which protein. Deletion analysis of B128 showed gene required for motility soft agar medium, but mutant displayed reduced growth rate increased sensitivity to bacitracin, antibiotic normally excluded by outer membrane. Introducing plasmid-borne...
BenM is a LysR-type bacterial transcriptional regulator that controls aromatic compound degradation in Acinetobacter sp. ADP1. Here, vitro transcription assays demonstrated two metabolites of catabolism, benzoate and cis,cis -muconate, act synergistically to activate gene expression. The level BenM-regulated benA was significantly higher response both compounds than the combined levels due each alone. These also were more effective together they individually altering DNase I footprint...
Rhizobium meliloti C4-dicarboxylic acid transport protein D (DCTD) activates transcription by a form of RNA polymerase holoenzyme that has sigma 54 as its factor (referred to E 54). DCTD catalyzes the ATP-dependent isomerization closed complexes between and dctA promoter transcriptionally productive open complexes. Transcriptional activation probably involves specific protein-protein interactions 54. Interactions 54-dependent activators are transient, there been no report biochemical assay...
The dctD gene product (DCTD) activates transcription from dctA by the sigma 54-holoenzyme form of RNA polymerase in Rhizobium meliloti. We have purified a constitutively active R. meliloti DCTD that lacks 142 amino acid residues N terminus (designated DCTDL143). Purified DCTDL143 recognized DCTD-binding sites at promoter region and catalyzed isomerization closed complexes between to open complexes. Like related 54-dependent activators NTRC NIFA, purine nucleoside triphosphate with...
We have detected activity of the nitrogen fixation regulatory protein NIFA Klebsiella pneumoniae in vitro. To do so we directed synthesis a coupled transcription-translation system and its ability to activate expression translational fusion between nifH lacZ genes. infer that stimulates initiation transcription by sigma 54 holoenzyme from nifHDK promoter. The was lost rapidly under both aerobic anaerobic conditions at 30 degrees C somewhat less 0 C. Loss not accompanied degradation...
Kineococcus radiotolerans SRS30216 was isolated from a high-level radioactive environment at the Savannah River Site (SRS) and exhibits gamma-radiation resistance approaching that of Deinococcus radiodurans. The genome sequenced by U.S. Department Energy's Joint Genome Institute which suggested existence three replicons, 4.76 Mb linear chromosome, 0.18 plasmid, 12.92 Kb circular plasmid. Southern hybridization confirmed chromosome is linear. K. sequence examined to learn about physiology...
The dimorphic prosthecate bacteria (DPB) are alpha-proteobacteria that reproduce in an asymmetric manner rather than by binary fission and of interest as simple models development. Prior to this work, the only member group for which genome sequence was available model freshwater organism Caulobacter crescentus. Here we describe Hyphomonas neptunium, a marine DPB differs from C. crescentus H. neptunium uses its stalk reproductive structure. Genome analysis indicates shares more genes with it...
Abstract Background Sigma54, or RpoN, is an alternative σ factor found widely in eubacteria. A significant complication analysis of the global 54 regulon a bacterium that RNA polymerase holoenzyme requires interaction with active bacterial enhancer-binding protein (bEBP) to initiate transcription at -dependent promoter. Many bacteria possess multiple bEBPs, which are activated by diverse environmental stimuli. In this work, we assess ability promiscuous, constitutively-active bEBP—the AAA+...
Helicobacter pylori FlgR activates transcription with sigma54-RNA polymerase holoenzyme (sigma54-holoenzyme) from at least five flagellar operons. Activators of sigma54-holoenzyme generally bind enhancer sequences located >70 bp upstream the promoter and contact bound through DNA looping to activate transcription. H. lacks carboxy-terminal DNA-binding domain present in most sigma54-dependent activators. As little as 42 flaB 26 sequence downstream transcriptional start site were sufficient...