A5064451549- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Animal Genetics and Reproduction
- Cardiac Fibrosis and Remodeling
- Atherosclerosis and Cardiovascular Diseases
- RNA Research and Splicing
- Long-Term Effects of COVID-19
- Coronary Interventions and Diagnostics
- Genetics, Aging, and Longevity in Model Organisms
- Innovation and Socioeconomic Development
- Bioinformatics and Genomic Networks
- Chromosomal and Genetic Variations
- Gene expression and cancer classification
- Extracellular vesicles in disease
- Genetic Neurodegenerative Diseases
- Transgenic Plants and Applications
- Dermatology and Skin Diseases
- Hair Growth and Disorders
- Biosensors and Analytical Detection
- melanin and skin pigmentation
Universitätsklinikum Aachen
2023-2024
University of Cologne
2018-2024
RWTH Aachen University
2022-2024
Bielefeld University
2019
Nanyang Technological University
2018
Fibrosis represents the common end stage of chronic organ injury independent initial insult, destroying tissue architecture and driving failure. Here we discover a population profibrotic macrophages marked by expression Spp1, Fn1, Arg1 (termed Spp1 macrophages), which expands after injury. Using an unbiased approach, identify chemokine (C-X-C motif) ligand 4 (CXCL4) to be among top upregulated genes during macrophage differentiation. In vitro in vivo studies show that loss Cxcl4 abrogates...
Biosensors have emerged as a valuable tool with high specificity and sensitivity for fast reliable detection of hazardous substances in drinking water. Numerous been addressed using synthetic biology approaches. However, many proposed biosensors are based on living, genetically modified organisms therefore limited shelf life, usability biosafety. We these issues by the construction an extensible, cell-free biosensor. Storage is possible through freeze drying paper. Following addition aqueous...
While CRISPR-Cas systems hold tremendous potential for engineering the human genome, it is unclear how well each system performs against one another in both non-homologous end joining (NHEJ)-mediated and homology-directed repair (HDR)-mediated genome editing.
Abstract Poised enhancers (PEs) represent a genetically distinct set of distal regulatory elements that control the expression major developmental genes. Before becoming activated in differentiating cells, PEs are already bookmarked pluripotent cells with unique chromatin and topological features could contribute to their privileged properties. However, since were originally characterized embryonic stem (ESC), it is currently unknown whether functionally conserved vivo. Here, we show 3D...
Abstract Germline specification in mammals occurs through an inductive process whereby competent cells the post-implantation epiblast differentiate into primordial germ (PGC). The intrinsic factors that endow with competence to respond germline signals remain unknown. Single-cell RNA sequencing across multiple stages of vitro PGC-like (PGCLC) differentiation system shows PGCLC genes initially expressed naïve pluripotent stage become homogeneously dismantled like-cells (EpiLC). In contrast,...
Introduction SARS-CoV-2 infection results in varying disease severity, ranging from asymptomatic to severe illness. A detailed understanding of the immune response is critical unravel causative factors underlying differences severity and develop optimal vaccines against new variants. Methods We combined single-cell RNA T cell receptor sequencing with CITE-seq antibodies characterize CD8 + at high resolution compared responses between mild COVID-19. Results observed increased exhaustion...
MYC plays various roles in pluripotent stem cells, including the promotion of somatic cell reprogramming to pluripotency, regulation competition and control embryonic diapause. However, how Myc expression is regulated this context remains unknown. The gene lies within a ~ 3-megabase desert with multiple cis-regulatory elements. Here we use genomic rearrangements, transgenesis targeted mutation analyse early mouse embryos cells. We identify topologically-associated region that homes enhancers...
Abstract Single-cell RNA sequencing (scRNA-seq) transcriptomics improves our understanding of cellular heterogeneity in healthy and pathological states. However, most scRNA-seq analyses remain confined to single cells or distinct cell populations, limiting their clinical applicability. Addressing the need translate single-cell insights into a patient-level disease understanding, we introduce mcBERT, new method that leverages data transformer-based model generate integrative patient...
Abstract Atherosclerosis is a pervasive contributor to cardiovascular diseases including ischemic heart disease and stroke. Despite the advance success of effective lipid lowering-therapies hypertensive agents, residual risk an atherosclerotic event remains high improving understanding development novel therapeutic strategies has proven be challenging. This largely due complexity atherosclerosis with spatial interplay multiple cell types within vascular wall. Here, we generated integrative...
SUMMARY Germline specification in mammals occurs through an inductive process whereby competent cells the post-implantation epiblast differentiate into primordial germ (PGC). The intrinsic factors that endow with competence to respond germline signals remain unknown. Single-cell RNA sequencing across multiple stages of vitro PGC-like (PGCLC) differentiation system shows PGCLC genes initially expressed naïve pluripotent stage become homogeneously dismantled like-cells (EpiLC). In contrast,...
Abstract Single cell RNA sequencing has provided unprecedented insights into the molecular cues and cellular heterogeneity underlying human disease. However, high costs complexity of single methods remain a major obstacle for generating large-scale cohorts. Here, we compare current state-of-the-art multiplexing technologies, provide widely applicable demultiplexing method, SoupLadle , that enables simple, yet robust high-throughput leveraging genetic variability patients.
BOFS is a rare congenital syndrome that arises due to defects during neural crest (NC) development. All reported cases are caused by heterozygous mutations within TFAP2A. Here we describe unique patient carrying de novo inversion in which one of the breakpoints located 40 kb downstream Using vitro and vivo NC developmental models, uncovered TFAP2A large Topologically Associating Domain (TAD) containing enhancers essential for expression cells (NCC). Importantly, using patient-specific hiPSC,...
Abstract Poised enhancers (PEs) represent a limited and genetically distinct set of distal regulatory elements that control the induction developmental genes in hierarchical non-redundant manner. Before becoming activated differentiating cells, PEs are already bookmarked pluripotent cells with unique chromatin topological features could contribute to their privileged properties. However, since were originally identified subsequently characterized using embryonic stem (ESC) as an vitro...