A5082323737- Mitochondrial Function and Pathology
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Amino Acid Enzymes and Metabolism
- ATP Synthase and ATPases Research
- Adipose Tissue and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Lipid Membrane Structure and Behavior
- Enzyme Structure and Function
- Metabolism, Diabetes, and Cancer
- Neuroscience and Neuropharmacology Research
- Wastewater Treatment and Nitrogen Removal
- Microbial Fuel Cells and Bioremediation
- Metalloenzymes and iron-sulfur proteins
- Autophagy in Disease and Therapy
- Molecular Sensors and Ion Detection
- Coenzyme Q10 studies and effects
- Ammonia Synthesis and Nitrogen Reduction
- Bioactive Compounds and Antitumor Agents
- Enzyme Catalysis and Immobilization
- Sulfur Compounds in Biology
- Cholinesterase and Neurodegenerative Diseases
- Electron Spin Resonance Studies
A N Bach Institute of Biochemistry
2010-2024
Russian Academy of Sciences
2010-2021
Federal Center Research Fundamentals of Biotechnology
2016-2021
Lomonosov Moscow State University
2012-2014
Alzheimer's disease (AD) is an incurable, age-related neurological disorder, the most common form of dementia. Considering that AD a multifactorial complex disease, simplified experimental models are required for its analysis. For this purpose, genetically modified Yarrowia lipolytica yeast strains expressing Aβ42 (the main biomarker AD), eGFP-Aβ42, Aβ40, and eGFP-Aβ40 were constructed examined. In contrast to cells eGFP eGFP-Aβ40, retaining "normal" mitochondrial reticulum, eGFP-Aβ42...
Benzalkonium chloride (BAC) is currently the most commonly used antimicrobial preservative in ophthalmic solutions, nasal sprays, and cosmetics. However, a large number of clinical experimental investigations showed that topical administration BAC-containing eye drops could cause variety ocular surface changes, from discomfort to potential risk for future glaucoma surgery. albuterol may increase albuterol-related systemic adverse effects. BAC, present personal care products, cosmetic...
Oxidative stress is involved in a wide range of age-related diseases. A critical role has been proposed for mitochondrial oxidative initiating or promoting these pathologies and the potential mitochondria-targeted antioxidants to fight them, making their search testing very urgent task. In this study, SkQ1, SkQ3 MitoQ were examined as they affected isolated rat liver mitochondria yeast cells, comparing with clinically tested SkQ1 MitoQ. At low concentrations, all three substances stimulated...
The Na(+)-coupled, high-affinity Pho89 plasma membrane phosphate transporter in Saccharomyces cerevisiae has so far been difficult to study because of its low activity and special properties. In this study, we have used a pho84Deltapho87Deltapho90Deltapho91Delta quadruple deletion strain S. devoid all genes specific for inorganic phosphate, except PHO89, functionally characterize under conditions where expression is hyperstimulated. Under these conditions, the protein strongly upregulated...
ABSTRACT The extracellular phosphate concentration permissive for the expression of different amounts active high-affinity Pho84 transporter in plasma membrane as well PHO84 messenger RNA levels low-phosphate-grown Saccharomyces cerevisiae cells is very narrow and essential a tight regulation transporter. undergoes rapid degradation once supply and/or carbon source exhausted.
Salt-excreting leaves of Atriplex halimus plants harvested in the central Negev Highlands Israel were screened for yeasts inhabiting their surfaces. Several aerobic, moderately salt- and alkali-tolerant isolated. One isolates (tentatively designated S-8) was identified as Yarrowia lipolytica (Wick.) van der Walt Arx, on basis its morphological, biochemical/physiological characteristics, quantitative chemotaxonomic molecular marker analyses. However, strain is distinguished from known members...
The Ca<sup>2+</sup> transport system of<i>Endomyces magnusii</i> mitochondria has been shown previously to be activated by spermine. Here we report it regulated also low, physiological ADP concentrations, the intramitochondrial NADH/NAD<sup>+</sup> ratio, and ions. combination of all these modulators induced high initial rates uptake Ca<sup>2+</sup>-buffering capacity yeast mitochondria, enabling them lower medium [Ca<sup>2+</sup>] ∼0.2 μm. mechanisms stimulation agents are discussed.