A5042471272- Growth Hormone and Insulin-like Growth Factors
- Education and Critical Thinking Development
- Cancer-related Molecular Pathways
- bioluminescence and chemiluminescence research
- Innovative Teaching Methods
- Listeria monocytogenes in Food Safety
- NF-κB Signaling Pathways
- Escherichia coli research studies
- Ocular Oncology and Treatments
- Salmonella and Campylobacter epidemiology
- Adipose Tissue and Metabolism
- interferon and immune responses
- Cell Image Analysis Techniques
- Cancer Research and Treatments
- Genomics and Chromatin Dynamics
- Online and Blended Learning
- Immune Response and Inflammation
- Higher Education Practises and Engagement
- International Student and Expatriate Challenges
- Mitochondrial Function and Pathology
- Pituitary Gland Disorders and Treatments
- Viral gastroenteritis research and epidemiology
- Amyotrophic Lateral Sclerosis Research
- Neurogenetic and Muscular Disorders Research
- CRISPR and Genetic Engineering
University of Liverpool
2010-2024
University of Manchester
2016
Tokyo Institute of Technology
2006
Dynamic cellular systems reprogram gene expression to ensure appropriate fate responses specific extracellular cues. Here we demonstrate that the dynamics of Nuclear Factor kappa B (NF-κB) signalling and cell cycle are prioritised differently depending on timing an inflammatory signal. Using iterative experimental computational analyses, show physical functional interactions between NF-κB E2 1 (E2F-1) 4 (E2F-4) regulators. These modulate response. In S-phase, response was delayed or...
A previously green fluorescent protein (GFP)-labeled PP01 virulent bacteriophage, specific to Escherichia coli O157:H7, was used construct lysozyme-inactivated GFP-labeled phage (PP01e-/GFP). The new recombinant lacked lytic activity because of the inactivation gene e, which produces lysozyme responsible for cell lysis. Gene e inactivated by inserting an amber stop codon. Prolonged incubation ofE. O157:H7 cells with PP01e-/GFP did not lead lysis, while propagation in host increased intensity...
PERP (p53 apoptosis effector related to PMP-22), a transcriptional target of p53, is downregulated and contributes the impairment in uveal melanoma (UM). Intriguingly, not induced UM despite functional p53. p63, located on chromosome 3, which characteristically altered high-risk UM, can transactivate PERP. Here, we determine role p63 expression initiation p53/PERP-mediated UM. was monitored by quantitative PCR (qPCR) immunoblotting cell lines treated with DNA-damaging agents. The assessed...
Abstract Amyotrophic lateral sclerosis (ALS) selectively affects motor neurons. SOD1 is the first causative gene to be identified for ALS and accounts at least 20% of familial (fALS) up 4% sporadic (sALS) cases globally with some geographical variability. The destabilisation dimer a key driving force in fALS sALS. Protein aggregation resulting from destabilised arrested by clinical drug ebselen its analogues (MR6-8-2 MR6-26-2) redeeming stability dimer. vitro target engagement these...
This paper presents the findings from a detailed study investigating UK undergraduate students’ experience of practical science in their post-16 studies during COVID-19 pandemic. It also examines perceived confidence and preparedness relation to areas skills at start courses. The employed an exploratory sequential mixed methods design. Findings student focus groups held end were used support development comprehensive audit quantitative survey for incoming students. Survey data collected...
Prolactin (PRL) is mainly expressed in the pituitary rodents, whereas humans, expression observed many extrapituitary sites, including lymphocytes. Due to lack of adequate experimental models, function locally produced PRL immune system largely unknown. Using transgenic rats that express luciferase under control extensive human regulatory regions, we characterized cell responses thioglycollate (TG)-induced peritonitis. Resident populations myeloid cells peritoneal cavity untreated barely...
Abstract Gene transcription occurs in short bursts interspersed with silent periods, and these kinetics can be altered by promoter structure. The effect of alternate architecture on bursting is not known. We studied the human prolactin (hPRL) gene that contains 2 promoters, a pituitary-specific requires factor Pit-1 displays dramatic transcriptional activity an upstream active nonpituitary tissues. large hPRL genomic fragments luciferase reporters, used bacterial artificial chromosome...
Abstract Intracellular protein translocation plays a pivotal role in regulating complex biological processes, including cell death. The tumor suppressor p53 is transcription factor activated by DNA damage and oxidative stress that also translocates from the cytosol into mitochondrial matrix to facilitate necrotic However, specific inhibitors of are largely unknown. To explore p53, we developed bioluminescent probe monitor mitochondria using luciferase fragment complementation assays....
Demonstrators spend significant time with students on a weekly basis in instructional laboratories and are well poised to offer meaningful learning. Most often, effective demonstrator training is neglected due resource restraints it clear more attention needed. We hypothesized that students' learning experience would improve if demonstrators were trained particularly across three overlapping domains: subject-specific knowledge (cognitive psychomotor), problem solving (cognitive) group...
This paper presents the findings from a detailed study investigating UK undergraduate students’ experience of practical science in their post-16 studies during COVID-19 pandemic. It also examines perceived confidence and preparedness students relation to areas skills at start degree courses. The employed an exploratory sequential mixed methods design, with focus groups end used support development comprehensive audit quantitative survey for incoming students. Survey data were collected...
Chinese students are the largest cohort of international in U.K., and community among University Liverpool students. This study aimed to assess students’ reasons for studying at their degree choices, awareness understanding employability skills, engagement with skills development. The results showed that our have a good what needed future careers. However, they also highlighted subject knowledge performance still seen as major requirements postgraduate employment, view is not necessarily...