A5000851248- Liver Disease Diagnosis and Treatment
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Nerve injury and regeneration
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Cellular Mechanics and Interactions
- Diet, Metabolism, and Disease
- Graphene and Nanomaterials Applications
- Alcohol Consumption and Health Effects
- Proteoglycans and glycosaminoglycans research
- Liver Disease and Transplantation
- Chronic Lymphocytic Leukemia Research
- Neurogenesis and neuroplasticity mechanisms
- Cancer Genomics and Diagnostics
- Acute Myeloid Leukemia Research
- Polymer Surface Interaction Studies
- Lymphoma Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Ferroptosis and cancer prognosis
- Viral-associated cancers and disorders
- Peripheral Neuropathies and Disorders
- Hepatitis C virus research
- Nanofabrication and Lithography Techniques
- Stroke Rehabilitation and Recovery
California Pacific Medical Center
2023-2025
University of California, San Francisco
2006-2024
San Francisco General Hospital
2023-2024
University of California, Berkeley
2006-2021
John H. Stroger, Jr. Hospital of Cook County
2020
University of South Florida
2019
Lake Erie College of Osteopathic Medicine
2018
University of California, Los Angeles
2018
Rutgers New Jersey Medical School
2011-2017
Rutgers, The State University of New Jersey
2017
Biodegradable nanofibers have tremendous potential for tissue repair. However, the combined effects of nanofiber organization and immobilized bioactive factors on cell guidance are not well understood. In this study, we developed aligned nanofibrous scaffolds by immobilizing extracellular matrix protein growth factor onto nanofibers, which simulated physical biochemical properties native fibrils. The significantly induced neurite outgrowth enhanced skin migration during wound healing...
Skeletal muscle consists of parallel bundles myotubes formed by the fusion myoblasts. We fabricated nanofibrous and micropatterned polymers as cell culture substrates to guide morphogenesis muscular tissue. The nanoscale microscale topographic features regulate cytoskeleton alignment, myotube assembly, striation, myoblast proliferation. This bottom-up approach from tissue level demonstrates potential for engineering assembly structure.
Abstract Endothelial cell (EC) migration is critical in wound healing and angiogenesis. Fluid shear stress due to blood flow plays an important role EC migration. However, the of surface heparan sulfate proteoglycans (HSPGs) adhesion, migration, mechanotransduction not well understood. Here, we investigated effects HSPG disruption on ECs cultured fibronectin. We showed that HSPGs with heparinase decreased adhesion rate by 40% strength 33%. At molecular level, fibers size focal adhesions...
Trauma injuries often cause peripheral nerve damage and disability. A goal in neural tissue engineering is to develop synthetic conduits for regeneration having therapeutic efficacy comparable that of autografts. Nanofibrous with aligned nanofibers have been shown promote regeneration, but current fabrication methods rely on rolling a fibrous sheet into the shape conduit, which results graft inconsistent size discontinuous joint or seam. In addition, long-term effects nanofibrous conduits,...
Human embryonic stem cell (ESC)-derived neural cells are a potential source for tissue regeneration. Understanding the biochemical and biophysical regulation of differentiation axon growth will help us develop therapies bioactive scaffolds. We demonstrated that basic fibroblast factor (bFGF) epidermal (EGF) had different effects on human ESC into cells. EGF was more effective in inducing expression neuron glial markers extensions. In addition to cues, poly(l-lactic acid) scaffolds with...
Abstract In regenerative medicine applications, the differentiation stage of implanted stem cells must be optimized to control cell fate and enhance therapeutic efficacy. We investigated potential human induced pluripotent (iPSC)-derived at two stages on peripheral nerve regeneration. Neural crest (NCSCs) Schwann (NCSC-SCs) derived from iPSCs were used construct a tissue-engineered conduit that was applied bridge injured nerves in rat sciatic transection model. Upon implantation, NCSC group...
<div>AbstractBackground:<p>Hepatocellular carcinoma (HCC) disproportionately affects racial/ethnic minorities. We evaluated the impact of income and geography on disparities across HCC care cascade in United States.</p>Methods:<p>Using NCI registry data spanning 2000 to 2020, adults with were determine race/ethnicity-specific differences tumor stage at diagnosis, delays gaps treatment, survival. Adjusted regression models predictors...
<p>Supplementary figure 1A shows the overall 5-year survival of patients with HCC stratified by age groups. Supplementary 1B sex. 1C race/ethnicity. 1D geography. 1E annual household income.</p>
Abstract Background Alcohol use and hepatitis C virus (HCV) often coexist are associated with cardiovascular disease. One of the underlying drivers is dyslipidemia. We assessed lipid lipoprotein levels relationship between alcohol atherogenic profiles, specifically small dense low‐density cholesterol (sdLDL‐C), in Latinos without HCV. Methods From June 1, 2002, to January 2016, 150 Latino adults underwent demographic, clinical, metabolic, lipid/lipoprotein, genetic evaluations. Linear...
Abstract The difficulty in spinal cord regeneration is related to the inhibitory factors for axon growth and lack of appropriate guidance lesion region. Here scaffolds are developed with aligned nanofibers nerve drug delivery cord. Blended polymers including poly( L ‐lactic acid) (PLLA) poly(lactide‐ co ‐glycolide) (PLGA) used electrospin nanofibrous a two‐layer structure: inner layer random outer layer. Rolipram, small molecule that can enhance cAMP (cyclic adenosine monophosphate) activity...
Abstract Vascular endothelium plays an important role in preventing thrombogenesis. Bioactive molecules such as fibronectin‐derived peptide Gly‐Arg‐Gly‐Asp‐Ser‐Pro (GRGDSP) can be used to modify the surface of cardiovascular implants vascular grafts promote endothelialization. Here we conjugated GRGDSP nonfouling interpenetrating polymer network (IPN), and investigated effects immobilized on EC functions under static flow conditions at well‐defined densities (∼0 3 pmol/cm 2 ). adhesion...
Casein kinases regulate a wide range of cellular functions in eukaryotes, including phosphorylation proteins that are substrates for degradation via the ubiquitin-proteasome system (UPS). Our previous study demonstrated Fbp1, component SCF(FBP1) E3 ligase complex, was essential Cryptococcus virulence. Because Saccharomyces cerevisiae homolog Grr1, requires casein kinase I (Yck1 and Yck2) to phosphorylate its substrates, we investigated function neoformans. In this report, identified C....
Aim: To create a synthetic nanofibrous dural substitute that overcomes the limitations of current devices by enhancing healing via biomimetic nanoscale architecture and supporting both onlaid sutured implantation. Materials & methods: A custom electrospinning process was used to bilayer having aligned nanofibers on one side random other. Nanoscale verified using microscopy macroscale mechanical properties were investigated tensile testing. Biological response this device in vitro canine...