Huaning Zhang

ORCID: 0000-0003-1197-0152
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About
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Research Areas
  • Heat shock proteins research
  • Plant Stress Responses and Tolerance
  • Salmonella and Campylobacter epidemiology
  • Seed Germination and Physiology
  • Vitamin K Research Studies
  • Microbial Metabolic Engineering and Bioproduction
  • Aluminum toxicity and tolerance in plants and animals
  • thermodynamics and calorimetric analyses
  • Viral gastroenteritis research and epidemiology
  • Vibrio bacteria research studies
  • Listeria monocytogenes in Food Safety
  • Genetics, Aging, and Longevity in Model Organisms
  • Enzyme Structure and Function
  • RNA Research and Splicing
  • Antibiotic Resistance in Bacteria
  • Plant biochemistry and biosynthesis
  • Aluminum Alloy Microstructure Properties
  • RNA and protein synthesis mechanisms
  • Microstructure and mechanical properties
  • Plant Genetic and Mutation Studies
  • Power Systems and Technologies
  • Food Safety and Hygiene
  • Pharmacological Effects of Natural Compounds
  • Enterobacteriaceae and Cronobacter Research
  • Power Systems Fault Detection

Hebei Academy of Agriculture and Forestry Sciences
2014-2025

Shandong Center for Disease Control and Prevention
2016-2023

NARI Group (China)
2019-2023

Shandong University
2016-2021

Shandong Academy of Environmental Science
2020

Hebei North University
2018

Hebei Normal University
2018

Centers for Disease Control and Prevention
2014

Tianjin Normal University
2014

Stony Brook University
2006-2011

Abstract Heat shock transcription factor (Hsf) plays a transcriptional regulatory role in plants during heat stress and other abiotic stresses. 31 non-redundant ZmHsf genes from maize were identified clustered the reference genome sequenced by Single Molecule Real Time (SMRT). The amino acid length, chromosome location, presence of functional domains motifs all ZmHsfs sequences analyzed determined. Phylogenetics collinearity analyses reveal gene duplication events Hsf family blocks shared...

10.1038/s41598-020-65068-z article EN cc-by Scientific Reports 2020-05-15

MenB, the 1,4-dihydroxy-2-naphthoyl-CoA synthase from bacterial menaquinone biosynthesis pathway, catalyzes an intramolecular Claisen condensation (Dieckmann reaction) in which electrophile is unactivated carboxylic acid. Mechanistic studies on this crotonase family member have been hindered by partial active site disorder existing MenB X-ray structures. In current work 2.0 Å structure of O-succinylbenzoyl-aminoCoA (OSB-NCoA) bound to Escherichia coli provides important insight into...

10.1021/bi200877x article EN Biochemistry 2011-08-10

Masson pine (Pinus massoniana) is one of the most important timber species with adaptable, fast growing, versatile advantages in southern China. Despite considerable research efforts, cellular and molecular mechanisms A1 toxicity resistance P. massoniana are still poorly understood. The effects Al on uptake translocation other minerals, cell division nucleolus were investigated.

10.1186/s12870-014-0264-9 article EN cc-by BMC Plant Biology 2014-09-29

Based on the information of 25 heat shock transcription factor (Hsf) homologues in maize according to a genome-wide analysis, ZmHsf06 was cloned from leaves and transformed into Arabidopsis thaliana (L. Heynh.) (ecotype, Col-0). Three transgenic positive lines were selected assess basic acquired thermotolerance drought-stress tolerance under stresses for some physiological assays. The sequence analysis indicates that contained characteristic domains class A type plant Hsfs. results qRT–PCR...

10.1071/fp15080 article EN Functional Plant Biology 2015-01-01

High temperature has severely affected plant growth and development, resulting in reduced production of crops worldwide, especially wheat. Alternative splicing (AS), a crucial post-transcriptional regulatory mechanism, is involved the development eukaryotes adaptation to environmental changes. Previous transcriptome data suggested that heat shock transcription factor (Hsf) TaHsfA2-7 may form different transcripts by AS. However, it remains unclear whether this mechanism related...

10.3390/ijms24021014 article EN International Journal of Molecular Sciences 2023-01-05

In this study, we were dedicated to investigating the effect caused by heat stress on wheat flag leaves. Metabolome and transcriptome analysis introduced identify some key biological processes. As a result, 182 214 metabolites significantly changed at anthesis post-anthesis stages, respectively; most of them lipids, amino acids derivatives, phenolic acids, alkaloids. Aminoacyl-tRNA biosynthesis was enriched pathway both two each which included 13 types acid, 12 shared up-regulated....

10.3390/ijms26041468 article EN International Journal of Molecular Sciences 2025-02-10

The dramatically high temperatures triggered by global climate change threaten maize growth and yield. In recent years, increasing attention has focused on the impacts of heat injury maize. However, molecular mechanisms behind maize’s adaptation to stress remain largely unexplored. To uncover how plants protect themselves from stress, we performed a phosphoproteomic analysis leaves using multiplex iTRAQ-based quantitative proteomic LC-MS/MS methods. A total 1594 phosphopeptides ascribed 875...

10.3390/ijms26062439 article EN International Journal of Molecular Sciences 2025-03-09

Abstract Background Heat shock transcription factors ( Hsf s) are present in majority of plants and play central roles thermotolerance, transgenerational thermomemory, many other stress responses. Our previous paper identified at least 82 H sf members a genome-wide study on wheat Triticum aestivum L.). In this study, we analyzed the expression profiles advanced development stages wheat, isolated markedly heat-responsive gene TaHsfA2–10 (GenBank accession number MK922287), characterized its...

10.1186/s12870-020-02555-5 article EN cc-by BMC Plant Biology 2020-08-03

A high-throughput screen led to the discovery of 2-amino-4-oxo-4-phenylbutanoate inhibitors 1,4-dihydroxy-2-naphthoyl-CoA synthase (MenB) from menaquinone biosynthesis pathway in Mycobacterium tuberculosis. However, these compounds are unstable solution and eliminate form corresponding 4-oxo-4-phenylbut-2-enoates that then react with CoA situ nanomolar MenB. The potency results interaction adduct carboxylate MenB oxyanion hole, a conserved structural motif crotonase superfamily....

10.1021/ml200141e article EN ACS Medicinal Chemistry Letters 2011-08-26

Rotavirus is the leading viral agent for pediatric gastroenteritis. However, case-based surveillance rotavirus limited in China, and its circulation environment not well investigated. From 2013 to 2014, was detected raw sewage samples of Jinan Linyi by quantitative PCR (qPCR) conventional reverse transcription (RT-PCR). After sequenced genotyped, sequences analysis conducted. A total 46 were collected monthly detection rotavirus, positive 43 (93.5 %, 43/46). By assessment, concentrations...

10.1186/s12985-016-0609-0 article EN cc-by Virology Journal 2016-09-13

Drought stress can seriously affect the yield and quality of wheat (Triticum aestivum). So far, although few heat shock transcription factors (Hsfs) have been found to be involved in response, biological functions them, especially members HsfC (heat factor C) subclass, remain largely unknown. Here, we identified a class C encoding gene, TaHsfC3-4, based on our previous omics data analyzed its function transgenic plants. TaHsfC3-4 encodes protein containing 274 amino acids shows basic...

10.3390/ijms25020977 article EN International Journal of Molecular Sciences 2024-01-12

Background Heat waves can critically influence maize crop yields. Plant heat shock transcription factors (HSFs) play a key regulating role in the (HS) signal transduction pathway. Method In this study, homologous cloning method was used to clone HSF gene ZmHsf01 (accession number: MK888854) from young leaves. The transcript levels of were detected using qRT-PCR different tissues and treated by HS, abscisic acid (ABA), hydrogen peroxide (H 2 O ), respectively, functions studied transgenic...

10.7717/peerj.8926 article EN cc-by PeerJ 2020-04-10

To study the toxic mechanisms of lead (Pb) in plants, effects Pb on morphology and structure nucleolus root tip meristematic cells Allium cepa var. agrogarum L. were investigated. Fluorescence labeling, silver-stained indirect immunofluorescent microscopy western blotting used. labeling showed that ions localized uptake accumulation increased with treatment time. At low concentrations (1–10 μM) there persistent nucleoli some during mitosis, at high concentration (100 many nucleolar...

10.3390/ijms150813406 article EN cc-by International Journal of Molecular Sciences 2014-07-31
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