Background Using antibody/aptamer-drug conjugates can be a promising method for decreasing toxicity, while increasing the efficiency of chemotherapy. Methodology/Principal Findings In this study, antitumor agent Doxorubicin (Dox) was incorporated into modified DNA aptamer TLS11a-GC, which specifically targets LH86, human hepatocellular carcinoma cell line. Cell viability tests demonstrated that TLS11a-GC-Dox exhibited both potency and target specificity. Importantly, intercalating Dox...

We have recently identified miR-125b upregulation in glioblastoma (GMB). The aim of this study is to determine the correlation between expression and malignant grades glioma genes targeted by miR-125b. Real-time PCR was employed measure level Cell viability evaluated cell growth colony formation soft-agar assays. apoptosis determined Hoechst 33342 staining AnnexinV-FITC assay. Luciferase assay used confirm actual binding sites p38MAPK mRNA. Western blot detect gene level. positively...

Sorafenib has been used to treat advanced hepatocellular carcinoma (HCC), but the underlying molecular mechanisms remain controversial and why some patients do not respond this therapy is poorly understood. In study, we show that sorafenib triggers cell growth inhibition apoptosis in HCC cells by directly targeting mitochondria. Treatment with induces rapid mitochondrial fragmentation, which associated deregulation of mitochondria fusion-related protein optic atrophy 1 (OPA1). Exposure or...

N 6 -methyladenosine [m(6)A/m A] methylation is one of the most common RNA modifications in eukaryotic cell mRNA and plays an important regulatory role metabolism, splicing, translocation, stability, translation. Previous studies have demonstrated that m A modification highly associated with tumor proliferation, migration, invasion. In present study, five factors been revealed, namely heterogeneous nuclear ribonucleoprotein A2/B1(HNRNPA2B1), C (HNRNPC), Vir like methyltransferase protein...

The factors behind the pathogenesis of lung cancer are not clear, and treatment failure is generally caused by drug resistance, recurrence, metastasis. Development new therapeutic agents to overcome drug-resistance remains a challenge clinically. Various extracts Foeniculum vulgare have shown promising anticancer activity; however, effects on underlying molecular mechanisms action clear. In present study, we found that ethanol extract seeds (EEFS) significantly reduced cell growth in vitro...

We present a practical synthesis of 2’-hydroxy-3’,4’,5’,6’-tetramethoxyacetophenone, multi-substituted benzene derivative, via CuCl-catalyzed Ullmann-type coupling reaction with MeONa. 2’-Hydroxy-3’,4’,5’,6’-tetramethoxyacetophenone is the key intermediate for total various polymethoxyflavones....

Our study aims to the therapeutic effects of a novel Bcl-2 inhibitor, ABT-263, on hepatocellular carcinoma (HCC) and provide primary preclinical data for future clinical trial with ABT-263. In this we showed that Bcl-xL survivin were up-regulated in HCC cell lines human liver cancer tissues. Clinic used ABT-263 single treatment had no apoptotic cells whereas higher doses did. Interestingly, combination inhibitor YM-155 could result significant apoptosis cells. Survivin inhibition through...

Dandelion is commonly used in traditional Chinese medicine with several active compounds found extracts. It has a variety of pharmacological effects, such as reduction swelling and inflammation, detoxification. The mechanism by which dandelion extract inhibits the inflammatory response skeletal muscle cells remains unknown; therefore, aim this study was to investigate effects root on proliferation alleviation lipopolysaccharide (LPS)-induced vitro.Rat were isolated from Sprague-Dawley rat...

Radiofrequency ablation (RFA) is the first-line option for early-stage hepatocellular carcinoma (HCC). However, residual tumor attributed to insufficient RFA (iRFA) led recurrence and metastasis. Novel combination strategies are urgently needed enhance efficiency of RFA. For in vitro iRFA models, HCC cells were placed a water bath at 46 °C 10 min then returned original incubator. vivo implanted subcutaneously into nude mice. The mice randomly assigned 4 groups: control group, XL888 group....

<italic>Foeniculum vulgare</italic> seed extract (FVE) exerts anti-cancer effects on hepatocellular carcinoma <italic>via</italic> reducing survivin protein levels and triggering mitochondrial toxicity.

Transforming growth factor-β (TGF-β) exerts apoptotic effects on various types of malignant cells, including liver cancer cells. However, the precise mechanisms by which TGF-β induces apoptosis remain poorly known. In present study, we have showed that threonine 32 (Thr32) residue FoxO3 is critical for to induce via Bim in hepatocarcinoma Hep3B Our data demonstrated induced activation through specific de-phosphorylation at Thr32. TGF-β-activated cooperated with Smad2/3 mediate up-regulation...

Abstract Despite much effort, pancreatic cancer survival rates are still dismally low. Novel therapeutics may hold the key to improving survival. YM155 is a small molecule inhibitor that has shown antitumor activity in number of cancers by reducing expression survivin. The aim our study understand mechanisms which functions cells. We established effect with vitro studies cultured cells, and vivo using mouse xenograft model. Our data demonstrated reduced survivin; however, downregulation...

Cholangiocarcinoma (CCA) is a relatively rare, heterogeneous malignant tumor with poor clinical outcomes. Because of high insensitivity to chemotherapy and radiotherapy, there are no effective treatment options. Efforts identify develop new agents for prevention this deadly disease urgent. Here, we assessed the apoptotic cytotoxicity Resina Draconis extract (RDE) using in vitro vivo assays identified mechanisms underlying antitumor effects RDE. RDE was obtained via vacuum distillation 75 %...

Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide. Herbal medicines have become an important treasure reservoir for anti-HCC drugs because their high efficiency and low toxicity. Herein, we investigated whether a 75% ethanol extract from Resina Draconis (ERD) exhibited comprehensive effects both in vivo vitro. We revealed that ERD effectively inhibited proliferation triggered apoptosis HCC cells dose- time-dependent maner, posing no apparent apoptotic...

Pancreatic cancer (PaCa), a common and highly lethal malignant cancer, is often insensitive to radio- and/or chemotherapy. Therefore, effective treatment regiments are still lacking. Herein, we found that an extract of Ficus carica fruit (EFCF) exerted anti-tumor effects on PaCa cells. EFCF induced cell viability inhibition apoptotic death in two lines dose- time dependent manner. effectively suppressed the migration, metastasis, invasion, colony formation Mechanistically, stimulated...

We determined the occurrence of single nucleotide polymorphisms (SNPs) -403A/G and -28C/G in promoter region RANTES 1082 Chinese blood donors from northern southern China 249 HIV patients China. Compared to healthy adults, AIDS had a significantly higher frequency -403G allele haplotype I, -403G/-28C (P < 0.05), lower -403A/A genotype 0.01). Symptomatic -28G -28C/C or = The plasma level was homozygous for I than those who were haplotypes II III 0.05). found be indigenous Africans, but...