Preparative agarose gel electrophoresis under denaturing conditions has been successfully employed to purify large quantities of ovalbumin mRNA from hen oviducts. The thus prepared is physically homogeneous based on its migration as a single component in both analytical acid-urea gels and formamide-containing, neutral polyacrylaminde gels; it also sediments peak sucrose gradients containing 70% formamide. chemically free ribosomal RNA contamination since oligonucleotide fingerprint map after...

Objectives Heart rate variability (HRV) has gained recognition as a noninvasive marker of autonomic activity. HRV is considered promising tool in various clinical scenarios. The optimal electrocardiogram (ECG) sampling frequency required to ensure sufficient precision R–R intervals for analysis not yet been determined. Here, we aimed determine the acceptable ECG range by analyzing signals from patients who visited an emergency department with chief complaint acute intoxication or overdose....

Five distinct 7-8 S small nuclear RNAs from Novikoff hepatoma were characterized by fingerprinting and their subcellular localization.Two of these (7-1 7-2) not found earlier.A 7 RNA previously reported to be localized cytoplasm was in nuclei amounts 30-50% the total.RNA 7-3 nucleoplasmic -different RNA.Several RNAs, 7-1, 7-2, 8 A, B, nucleoli.The 7-1 , B released nucleolar 28 extracted at 25 "C heating 65 "C.The TI RNase fingerprints A very similar.The similarities HeLa cells support...

When comparing mortality, the severity of illness or injury should be considered; therefore, scoring systems that represent have been developed and used. Given diagnosis codes in International Classification Disease (ICD) vital signs are part routine data used medical care, a system based on these would allow for comparison severity-adjusted treatment outcomes without substantial additional efforts. This study was National Emergency Department Information System database Republic Korea....

Abstract Paraneoplastic pemphigus ( PNP ) is a rare, life‐threatening, autoimmune, mucocutaneous blistering disease associated with neoplasia. Both humoral and cellular immunity are involved in the pathogenesis of . Characteristically, has diverse spectrum clinical immunopathological features. We retrospectively analyzed 12 K orean patients who were diagnosed between 1993 2011. performed analysis features, outcomes, underlying neoplasia, histological features laboratory findings. All except...

To evaluate the long-term outcomes of rituximab in treatment pemphigus and influence disease duration different dose on clinical response, 45 patients with refractory treated at least one cycle two infusions (375 mg/m2 per infusion weekly) were retrospectively studied. All followed up for more than 2 years. achieved complete or partial remission within 8 months first cycle. Thirty-four (76%) relapsed a median 17 months. who received additional cycles after relapse new remissions. Early use 1...

Comparisons were made of the T1 ribonuclease digests 32P-labeled nucleolar 45S RNA intact Novikoff hepatoma cells and synthesized in vitro by isolated nucleoli. Approximately 200 oligonucleotide spots found two-dimensional chromatogram labeled vivo, which includes fragments 18S 28S rRNA nonconserved spacer regions; four containing 2'-O-methyl nucleotides not corresponding pattern vitro. This high degree fidelity was retained patterns from produced with chromatin as template. specific...

Studies on the composition of newly-synthesized nucleolar and ribosomal RNA Novikoff rat hepatoma have demonstrated that a portion these molecules differs from corresponding similar normal liver These studies suggest genetic defect in cancer cells may be mirrored aberrations RNA. Accordingly, possibility exists modification this by uptake specific oligonucleotides localization genes involved its synthesis provide mechanism inhibition growth tumor cells. To test can take up intact or...

Collecting patient’s medical data is essential for emergency care. Although hospital-tethered personal health records (PHRs) can provide accurate data, they are not available as electronic information when the hospital does develop and supply PHRs. The objective of this research was to evaluate whether a mobile app assemble from different hospitals enable interoperability. Moreover, we identified numerous barriers overcome putting into one place. new PHR (mPHR) application developed...

The presence of heparin in cell culture medium was found to reduce the infectivity titers cytomegaloviruses isolated from human, monkey, guinea pig and mouse. This inhibition shown increase as concentration increased. When added cultures after virus adsorption had taken place, effect on negligible. Other anticoagulants studied included Alsever's solution, sodium citrate EDTA, none which showed any inhibitory cytomegalovirus replication culture.

This study analyzed the incidence and characteristics of in-hospital cardiac arrest (IHCA) in Korea based on a sample group patients that is representative population.The IHCA adults was extracted from HIRA-NIS-2009, all using medical services Korea. were according to gender, age, type institute, classification under 6th revision Korean Standard Classification Diseases (KCD-6). In addition, assess differences arising size institutes, relation number inpatient beds.Based data, total found be...

Mucous membrane pemphigoid (MMP) is a rare autoimmune, subepidermal, bullous disease characterized by erosive lesions on the mucous membranes and skin. MMP reacts with various target antigens including BP180, laminin-332, β4 integrin, α6 integrin or type VII collagen. We present 67-year-old male patient who had oral ocular facial By immunoblot analyses, immunoglobulin G autoantibodies in patient's sera reacted full-length BP180 120-kDa ectodomain of (LAD-1).

With the advent of next-generation sequencing (NGS), targeted is now contributing to decision making for which chemotherapeutics administer cancer patients, especially in refractory and metastatic cancer. Given that most patients with develop resistance chemotherapy have few treatment options, we performed NGS test evaluate efficacy clinical feasibility NGS-based anticancer therapy. We used a gene panel capturing target regions covering 83 cancer-related genes. A total 25 solid tumor were...

A nationwide strike that took place from August 21 to September 7, 2020, which was led by young doctors represented residents and interns, resulted in shortages of manpower at almost all university training hospitals. This study aimed identify differences the process outcomes emergency department (ED) patient care comparing performance over about 2 weeks with during usual ED operations. retrospective observational evaluated flow junior doctors' compared it period a single tertiary-care...

Emergency department (ED) overcrowding is a worldwide problem that poses threat to patient safety by causing treatment delays and increasing mortality. Consultations are common important in the emergency medicine profession associated with longer ED length of stay (LOS). The purpose this study was evaluate impact admission decisions physicians without consultations on LOS other quality indicators. retrospective observational comparing patients admitted internal (IM) before after policy...

Summary Background: Mapping of drug indications to ICD-10 was undertaken in Korea by a public and private institution for their own purposes. A different mapping approach used each institution, which presented good opportunity compare the validity two approaches. Objectives: This study direct an indirect terminology based against gold standard drawn from results processes. Methods: Three hundred seventy-five cardiovascular reference drugs were selected all listed study. In approach,...

Two complexes were isolated from 20 S ribonucleoprotein (RNP) fraction and 60 subunit.The RNP was found to contain the 3'-340 nucleotide fragment (domain VII) in association with 5 rRNA.The subunit contained a stable complex consisting of 5"upstream portion (4220-4462, domain VI VII), 3"downstream (4463-4802, 3'-583 nucleotides fragment, 5.8 rRNA.By computer analysis hybridization, 5'-upstream rRNA contact site.By affinity chromatography, 3'-downstream site.Furthermore, by comparison...

Personal health records (PHRs) can be useful in the emergency department, as they provide patient information an accurate and timely manner enable it to used actively. This has effect on patients' outcomes experience. Despite importance of PHRs emergencies, there are only a few studies related emergencies that evaluate experience.This study aims introduce novel mobile PHR (mPHR) platform environments assess user experience.The was conducted from October 2019 November 2019. In total, 1000...

The internal topography of rRNAs in situ was probed with RNase T1.Isolated polysomes were treated T1 to examine the unprotected regions contained polysomes.The 40 S subunit yielded 23 cleavage products 18 rRNA, which fragments from three domains isolated, characterized, and compared secondary structure rRNA proposed by Chan et al. (Chan, Y.-L., Gutell, R., Noller, H. F., Wool, I. G .(1984) J. Biol.Chem.259,224-230).There two consistent (1-71,l-74) derived 5' domain, where alternative sites...