A5035487254- Antifungal resistance and susceptibility
- Fungal and yeast genetics research
- Mycotoxins in Agriculture and Food
- Identification and Quantification in Food
- Food Supply Chain Traceability
- Environmental DNA in Biodiversity Studies
- Metabolism and Genetic Disorders
- Aquaculture disease management and microbiota
- Fungal Biology and Applications
- Folate and B Vitamins Research
- Isotope Analysis in Ecology
- Aquaculture Nutrition and Growth
- Fungal Infections and Studies
- Alcoholism and Thiamine Deficiency
- 14-3-3 protein interactions
- Cell Image Analysis Techniques
- Probiotics and Fermented Foods
- Nanoplatforms for cancer theranostics
- Microbial Natural Products and Biosynthesis
- Moringa oleifera research and applications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Advanced Chemical Sensor Technologies
- Microtubule and mitosis dynamics
- Analytical Chemistry and Sensors
- Smart Agriculture and AI
Nanjing Medical University
2014-2025
South China University of Technology
2022-2024
Guangzhou Medical University
2024
Guangzhou First People's Hospital
2024
Chinese Academy of Fishery Sciences
2024
Ministry of Agriculture and Rural Affairs
2024
Hunan Institute of Engineering
2023
North Minzu University
2023
Southern Medical University
2022
Nanjing Normal University
2012-2014
Mono-chemotherapy has significant side effects and unsatisfactory efficacy, limiting its clinical application. Therefore, a combination of multiple treatments is becoming more common in oncotherapy. Chemotherapy combined with the induction ferroptosis potential new Furthermore, polymeric nanoparticles (NPs) can improve antitumor efficacy decrease toxicity drugs. Herein, NP, mPEG-b-PPLGFc@Dox, synthesized to doxorubicin (Dox) enhance chemotherapy by combining it ferroptosis. First,...
Nitrite is the main environmental pollutant that endangers shrimp culture. Intestinal health essential for disease resistance of shrimp. In this study,
This study aims to identify lactic acid bacteria (LAB) isolates possessing physiological characteristics suitable for use as probiotics in yogurt fermentation. Following and bile salt tolerance tests, Lactiplantibacillus plantarum (NUC08 NUC101), Lacticaseibacillus rhamnosus (NUC55 NUC201), paracasei (NUC159, NUC216, NUC351) were shortlisted based on intraspecies distribution further evaluation. Their probiotic properties, including transit tolerance, adhesion, autoaggregation, surface...
Purpose: Current treatment approaches for Prostate cancer (PCa) often come with debilitating side effects and limited therapeutic outcomes. There is urgent need an alternative effective safe PCa. Methods: We developed a nanoplatform to target prostate cells based on graphdiyne (GDY) copper-based metal-organic framework (GDY-CuMOF), that carries the chemotherapy drug doxorubicin (DOX) treatment. Moreover, provide GDY-CuMOF@DOX homotypic targeting capability, we coated PCa cell membrane (DU145...
The increasing complexity of environmental pollution nowadays poses a severe threat to the public health, which attracts considerable attentions in searching for nanomaterials multiproperty. In this study, mesoporous silica KIT-6-encapsulated bismuth oxychloride (BiOCl), an intrinsically multifunctional material exhibiting bunched structure composites, are facilely prepared under hydrothermal conditions. Subsequently, produced materials multifunctionality were applied photocatalysis,...
Identification of meat authenticity is a matter increasing concerns due to religious, economical, legal, and public health reasons. However, little known about the inspection eight species in one tube reaction technological challenge multiplex polymerase chain (PCR) techniques. Here, developed PCR method can simultaneously authenticate including ostrich (753 bp), cat (564 goose (391 duck (347 chicken (268 horse (227 dog (190 sheep (131 bp). The detectable deoxyribonucleic acid (DNA) contents...
Lipoteichoic acid synthase (LtaS) is crucial for the biosynthesis of lipoteichoic (LTA) in lactic bacteria (LAB), where LTA plays a key role bacterial adhesion, immune modulation, and maintaining cell integrity. This study explores regulation LtaS activity Lactiplantibacillus plantarum, examining effects factors such as temperature, pH, metal ions on enzyme activity. Molecular docking was used to identify critical amino acids at enzyme's active site, site-directed mutagenesis confirmed five...
Summary Timely cytokinesis/septation is essential for hyphal growth and conidiation in A spergillus nidulans . Genetic analyses have identified that has components of the septum initiation network ( SIN ) pathway; one these, SEPH , a key player early events during cytokinesis. However, little known about how kinase cascade regulated by other components. Here, we demonstrate phosphoribosyl pyrophosphate synthetase family acts antagonistically against so downregulation AnPRS can bypass...
In this paper, an automatic colony counting system based on improved image preprocessing algorithm and convolutional neural network (CNN)-assisted method was developed. Firstly, we assembled LED backlighting illumination platform as capturing to obtain photographs of laboratory cultures. Consequently, a dataset introduced consisting 390 photos agar plate cultures, which included 8 microorganisms. Secondly, implemented new for light intensity correction, facilitated clearer differentiation...
Protein phosphatase 2A (PP2A) is a major intracellular protein that regulates multiple aspects of cell growth and metabolism. Different activities PP2A subcellular localization are determined by its regulatory subunits. Here we identified characterized the functions two subunit homologs, ParA PabA, in Aspergillus nidulans. Our results demonstrate localizes to septum site deletion parA causes hyperseptation, while overexpression abolishes formation; this suggests may function as negative...
Frequent meat frauds have aroused significant social attention. The aim of this study is to construct a two-tube hexaplex polymerase chain reaction (PCR) method offering accurate molecular authentication twelve species in actual adulteration event. Deoxyribonucleic acid (DNA) sequencing demonstrates that designed primers can specifically amplify target from genomic DNA mixture six each tube reaction, which showed 100% accuracy horse (148 bp), pigeon (218 camel (283 rabbit (370 ostrich (536...
Meat adulteration have become a global issue, which has increasingly raised concerns due to not only economic losses and religious issues, but also public safety its negative effects on human health. Using optimal primers for seven target species, multiplex PCR method was developed the molecular authentication of camel, cattle, dog, pig, chicken, sheep duck in one tube reaction. Species-specific amplification from premixed total DNA species corroborated by sequencing. The limit detection...
As an acute respiratory infectious disease, COVID-19 threatens the safety of global public health. Given current lack specific treatment against this research and development vaccines have become sharp weapons for overcoming pandemic. mRNA lead in vaccination strategies due to their advantages, such as rapid industrial production efficacy. A total 137 entered clinical trial stage, among which 23 are vaccines, accounting 17% vaccines. Herein, we summarize developmental processes well approach...
Phosphoribosyl pyrophosphate synthetase, which is encoded by the Prs gene, catalyses reaction of ribose-5-phosphate and adenine ribonucleotide triphosphate (ATP) has central importance in cellular metabolism. However, knowledge about how family members function contribute to total 5-phosphoribosyl-α-1-pyrophosphate (PRPP) synthetase activity limited. In this study, we identified that filamentous fungus Aspergillus nidulans genome contains three PRPP synthase-homologous genes (AnprsA, AnprsB...
Antifungal treatment is often ineffectual, partly because of biofilm formation. In this study, by using a combined forward and reverse genetic strategy, we identified that nucleus-localized AfSsn3 its partner AfSsn8, which constitute Cdk8-cyclin pair, are required for azole resistance in Aspergillus fumigatus Deletion Afssn3 led to increased absorption utilization glucose amino acids. Interestingly, accelerated the extracellular polysaccharide formation, while acids serine, threonine,...
ABSTRACT The efficacy of photothermal therapy (PTT) in antitumor applications is often limited by inadequate tumor targeting and low conversion efficiency (PCE) photosensitizers. In this study, we designed a nanoparticle, MPF@IR780, to enhance photosensitizers' PCE. First, MPF@IR780 improves the delivery photosensitizers tissue through enhanced permeability retention (EPR) effect. Furthermore, hydrophobic ferrocene was incorporated into nanoparticle core increase structural compactness,...
An efficient reactive oxygen species (ROS) detoxification system is vital for the survival of pathogenic fungus Aspergillus fumigatus within host high-ROS environment host. Therefore, identifying and targeting factors essential oxidative stress response one approach to developing novel treatments fungal infections. The oxidation resistance 1 (Oxr1) protein protection against in mammals, but its functions fungi remain unknown. present study aimed characterize role an Oxr1 homolog A....
Frequent meat frauds have become a global issue because adulteration risks the food safety, breaches market rules, and even threatens public health. Multiplex PCR is considered to be simple, fast, inexpensive technique that can applied for identification of products in industries. However, relatively less known about multiplex method authenticating seven animal species simultaneously one reaction due technological challenge. Through screening new species-specific primers optimizing system,...
Aspergillus fumigatus is a well-known opportunistic pathogen that causes invasive aspergillosis (IA) infections, which have high mortality rates in immunosuppressed individuals. Long-term antifungal drug azole use clinical treatment and agriculture results loss of efficacy or resistance. Drug resistance related to cellular metabolites the corresponding gene transcription. In this study, through untargeted metabolomics transcriptomics under itraconazole (ITC) treatment, we identified two...
In Aspergillus fumigatus, the most prevalent resistance to azoles results from mutational modifications of azole target protein Cyp51A, but there are non-cyp51A mutants resistant azoles, and mechanisms underlying these strains remain be explored. Here, we identified a novel cytochrome c oxidase, cox7c (W56*), nonsense mutation in laboratory found that it caused reduced colony growth multiantifungal agents. Meanwhile, revealed cold storage is responsible for increased tolerance conidia...
Aspergillus fumigatus is the most prevalent airborne fungal pathogen that causes invasive infections in immunosuppressed individuals. Adaptation to iron limited conditions crucial for A. virulence. To identify novel genes play roles adaptation low we performed an insertional mutagenesis screen fumigatus. Using this approach, identified tptA gene fumigatus, which shares homology with Saccharomyces cerevisiae thiamine pyrophosphate (ThPP) transporter encoding tpc1. Heterologous expression of...