A5044635068- Interconnection Networks and Systems
- Advanced Graph Theory Research
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Microbial Inactivation Methods
- RNA modifications and cancer
- VLSI and Analog Circuit Testing
- Phytochemicals and Antioxidant Activities
- Embedded Systems Design Techniques
- Radiation Effects in Electronics
- Gut microbiota and health
- SARS-CoV-2 and COVID-19 Research
- Graph theory and applications
- Cytokine Signaling Pathways and Interactions
- Diet and metabolism studies
- HIV Research and Treatment
- Inflammatory mediators and NSAID effects
- Postharvest Quality and Shelf Life Management
- interferon and immune responses
- Osteoarthritis Treatment and Mechanisms
- Global Health Care Issues
- Computational Geometry and Mesh Generation
- Air Quality and Health Impacts
- Metabolomics and Mass Spectrometry Studies
- Fungal Biology and Applications
Yale University
2022-2024
First Affiliated Hospital of Xinxiang Medical University
2023
Beijing Technology and Business University
2022-2023
University of Ottawa
2016-2022
Beijing Forestry University
2016-2022
Beijing Normal University
2015-2022
Xi'an Honghui Hospital
2022
Xi'an Jiaotong University
2022
Versus Arthritis
2022
Weatherford College
2022
Abstract The gut microbiota plays a critical role in obesity and lipid metabolism disorder. Chokeberry ( Aronia melanocarpa L . ) are rich polyphenols with various physiological pharmacological activities. We determined serum parameters fecal microbial components by using related kits, liquid chromatography-mass spectrometry (LC-MS) 16S rRNA gene sequencing every 10 days. Real-time PCR analysis was used to measure expression of bile acids (BAs) liver adipose tissues. analyzed the effects...
Abstract The degree to which codon usage can be explained by tRNA abundance in bacterial species is often inadequate, partly because differential approximated copy numbers. To better understand the coevolution between and usage, we provide a estimate of profiling mapped reads (tRNA tpm) using publicly available RNA Sequencing data. emphasize feasibility our approach, demonstrate that tpm consistent with abundances derived from fingerprinting experiments Escherichia coli , Bacillus subtilis...
Three stop codons in bacteria represent different translation termination signals, and their usage is expected to depend on differences efficiency, mutation bias, relative abundance of release factors (RF1 decoding UAA UAG, RF2 UGA). In 14 bacterial species (covering Proteobacteria, Firmicutes, Cyanobacteria, Actinobacteria Spirochetes) with cellular RF1 quantified, consistently over-represented highly expressed genes (HEGs) lowly (LEGs), whereas UGA the opposite even where far more abundant...
SARS-CoV-2 can transmit efficiently in humans, but it is less clear which other mammals are at risk of being infected. encodes a Spike (S) protein that binds to human ACE2 receptor mediate cell entry. A species with human-like could therefore be infected by SARS-CoV-2. We compared between 132 mammalian genes and 17 coronavirus S proteins. showed while global similarities reflected whole gene alignments poor predictors high-risk mammals, local key protein-binding sites highlight several share...
Coronaviruses such as SARS-CoV-2 regularly infect host tissues that express antiviral proteins (AVPs) in abundance. Understanding how they evolve to adapt or evade immune responses is important the effort control spread of infection. Two AVPs may shape viral genomes are zinc finger protein (ZAP) and apolipoprotein B mRNA editing enzyme-catalytic polypeptide-like 3 (APOBEC3). The former binds CpG dinucleotides facilitate degradation transcripts while latter frequently deaminates C into U...
Black chokeberry ( Aronia melanocarpa L.) is rich in polyphenols with various physiological and pharmacological activities. However, the relationship between modulation effect of black on obesity alteration lipid metabolism not clearly understood. This study aimed to investigate beneficial effects (BCPs) treatment structure gut microbiota, metabolism, associated mechanisms high-fat diet (HFD)-induced obese rats. Here, we found that a promoted body weight gain accumulation rats, while oral...
Trachelospermum jasminoides is commonly used in traditional Chinese medicine. However, the use of plant's local alternatives frequent, causing potential clinical problems. The T. sold medicine market dried and sliced, making identification methods difficult. In this study, ITS2 region was evaluated on 127 sequences representing its according to PCR sequencing rates, intra- inter-specific divergences, secondary structure, discrimination capacity. Results indicated 100% success rates obvious...
Bacterial translation initiation is influenced by base pairing between the Shine-Dalgarno (SD) sequence in 5' UTR of mRNA and anti-SD (aSD) at free 3' end 16S rRNA (3' TAIL) due to: 1) SD/aSD binding location 2) affinity. In order to understand what makes an interaction optimal, we must define: terminus TAIL extent core aSD within TAIL. Our approach characterize these components Escherichia coli Bacillus subtilis involves mapping boundary mature using high-throughput RNA sequencing...
Termination efficiency of stop codons depends on the first 3' flanking (+4) base in bacteria and eukaryotes. In both Escherichia coli Saccharomyces cerevisiae, termination read-through is reduced presence +4U; however, molecular mechanism underlying +4U function poorly understood. Here, we perform comparative genomics analysis 25 bacterial species (covering Actinobacteria, Bacteriodetes, Cyanobacteria, Deinococcus-Thermus, Firmicutes, Proteobacteria, Spirochaetae) with bioinformatics...
Piper kadsura is a vine-like medicinal plant which widely used in clinical treatment. However, P. often substituted by other materials the markets, thereby causing health risks. In this study, 38 samples and eight sequences from GenBank, including closely-related species common adulterants were collected. This study aimed to identify an effective DNA barcode four popular loci for authentication. The success rates of PCR amplification, sequencing, sequence acquisition matK 10.5%, 75%, 7.9%,...
The accurate identification and quality evaluation of herbal medical plants is highly necessary to ensure their safety efficacy. In present study, a new strategy combining DNA barcoding techniques with thin-layer chromatography (TLC) high-performance liquid (HPLC) was proposed facilitate the control M. tenacissima. work, internal transcribed spacer 2 (ITS2) barcode successfully used identify 58 tenacissima samples its adulterants. TLC identified other three that failed produce ITS2 regions....
Majority of protein structure studies use Escherichia coli (E. coli) and other model organisms as expression systems for species' genes. However, folding depends on cellular environment factors, such chaperone proteins, cytoplasmic pH, temperature, ionic concentrations. Because differences in these especially temperature chaperones, native proteins extremophiles may fold improperly when they are expressed mesophilic organisms. Here we present a methodology assessing the effects using E....