A5042631738- Adipose Tissue and Metabolism
- Cardiovascular Disease and Adiposity
- Cardiovascular Health and Disease Prevention
- Nitric Oxide and Endothelin Effects
- Hormonal Regulation and Hypertension
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Regulation of Appetite and Obesity
- Birth, Development, and Health
- Renin-Angiotensin System Studies
- Biochemical Analysis and Sensing Techniques
- Metabolism, Diabetes, and Cancer
- Diet, Metabolism, and Disease
- Diet and metabolism studies
- Dietary Effects on Health
- Blood Pressure and Hypertension Studies
- Eicosanoids and Hypertension Pharmacology
- Obesity, Physical Activity, Diet
- Protease and Inhibitor Mechanisms
- Medication Adherence and Compliance
- Antioxidant Activity and Oxidative Stress
- Apelin-related biomedical research
- Receptor Mechanisms and Signaling
- Connective Tissue Growth Factor Research
- Pregnancy and preeclampsia studies
Cornell University
2025
Universidad San Pablo CEU
2015-2024
Hospital General Universitario Gregorio Marañón
2021-2022
Universidad San Pablo
2012-2021
Universidad Complutense de Madrid
1996-2010
Universidad Autónoma de Madrid
2001-2008
Comisión Interministerial de Ciencia y Tecnología
2004
GTx (United States)
2004
University of the Andes
1980
University of Cincinnati
1980
Background The hypothesis of this study is that long-term high-fat diets (HFD) induce perivascular adipose tissue (PVAT) dysfunction characterized by a redox imbalance, which might contribute to aggravate endothelial in obesity. Methods and Results C57BL/6J mice were fed either control or HFD (45% kcal from fat) for 32 weeks. Body weight, lumbar mesenteric weights significantly higher animals compared controls. anticontractile effect PVAT arteries (MA) was lost after week...
Albuminuria is an early marker of renovascular damage associated to increase in oxidative stress. The Munich Wistar Frömter (MWF) rat a model chronic kidney disease (CKD), which exhibits endothelial dysfunction low nitric oxide availability. We hypothesize that the new highly selective, non-steroidal mineralocorticoid receptor (MR) antagonist, finerenone, reverses both and microalbuminuria. Twelve-week-old MWF (MWF-C; MWF-FIN) aged-matched normoalbuminuric (W-C; W-FIN) rats were treated with...
Chronic hypertension is associated with resistance artery remodelling and mechanical alterations. However, the contribution of elastin has not been thoroughly studied. Our objective was to evaluate role in vascular mesenteric arteries (MRA) from spontaneously hypertensive rats (SHR). MRA segments Wistar Kyoto (WKY) SHR were pressurised under passive conditions at a range physiological pressures pressure myography. Confocal microscopy used determine differences quantity organisation intact...
Perivascular adipose tissue (PVAT) plays a paracrine role in regulating vascular tone. We hypothesize that PVAT undergoes adaptative mechanisms during initial steps of diet-induced obesity (DIO) which contribute to preserve function. Four-week-old male C57BL/6J mice were assigned either control [low-fat (LF); 10% kcal from fat] or high-fat diet (HF; 45% fat). After 8 wk dietary treatment function was analyzed the whole perfused mesenteric bed (MB) and isolated arteries cleaned PVAT. Relaxant...
Leptin causes vasodilatation both by endothelium-dependent and -independent mechanisms. is synthesized perivascular adipose tissue (PVAT). The hypothesis of this study that a decrease leptin production in PVAT spontaneously hypertensive rats (SHR) might contribute to diminished paracrine anticontractile effect the hormone. We have determined aorta from Wistar-Kyoto (WKY) SHR (i) mRNA protein levels PVAT, (ii) on contractile responses, (iii) leptin-induced relaxation nitric oxide (NO)...
Activation of endothelial adenosine monophosphate-activated protein kinase (AMPK) contributes to increase nitric oxide (NO) availability. The aim this study was assess if high-fat diet (HFD)-induced dysfunction is linked AMPK deregulation.Twelve-week-old Sprague Dawley male rats were assigned either control (10 kcal % from fat) or HFD (45 for 8 wk. segregated in obesity-prone (OP) obesity-resistant (OR) according body weight. triggered an impaired glucose management together with...
Resistant albuminuria, developed under adequate chronic blockade of the renin-angiotensin system, is a clinical problem present in small number patients with kidney disease (CKD). The mechanism underlying this resistant albuminuria remains unknown. Matrix metalloproteinases (MMPs) are involved pathophysiology cardiovascular and renal diseases. In study we tested role MMPs albuminuria. First evaluated gelatinase MMP-2 MMP-9 activity by zymography Munich Wistar Frömter (MWF) rat, model...
The objective of this work was to characterize the adaptation cardiac metabolism a lipid overload in model diet-induced obesity (DIO) mice. After 8 wk dietary treatment, mice receiving high-fat diet exhibited an increase amount adipose tissue, accompanied by surge plasma leptin concentration (from 5.4–16.0 ng/ml). This associated with: 1) induction uncoupling protein-2 (120%), 2) phosphorylated form AMP-activated protein kinase and 3) reduction lactate dehydrogenase activity myocardial...
Fetal undernutrition is a risk factor for heart disease in both genders, despite the protection of women against hypertension development. Using rat model maternal (MUN) we aimed to assess possible sex differences development cardiac alterations and implication oxidative stress.Male female offspring from rats fed ad libitum (control) or with 50% normal daily intake during second half gestation were used. Heart weight/body weight ratio (HW/BW), hemodynamic parameters (anaesthetized rats)...
<b><i>Background:</i></b> Development of albuminuria and arterial stiffness in Munich Wistar Frömter (MWF) rats, a model chronic kidney disease, is related to alterations extracellular matrix, increased oxidative stress, endothelial dysfunction. Finerenone (FIN), novel, nonsteroidal, potent, selective mineralocorticoid receptor antagonist, improves dysfunction through enhancing nitric oxide (NO) bioavailability decreasing superoxide anion levels due an upregulation...
High-fat (HF) diets trigger an increase in adipose tissue and body weight (BW) disordered eating behavior. Our study deals with the hypothesis that circadian distribution of energy intake is more relevant for BW dynamics than diet composition. Four-week-old mice were exposed 8 wk to a HF compared animals receiving control chow. progressively increased BW, decreased amount nocturnal (1800-0900 h) calories (energy or food intake) (30%) diurnal (0900-1800 caloric intake), although total daily...
Caloric restriction (CR) ameliorates cardiac dysfunction associated with obesity. However, most of the studies have been performed under severe CR (30–65% caloric intake decrease) for several months or even years in aged animals. Here, we investigated whether mild (20% food reduction) and short-term (2-weeks) prevented obese cardiomyopathy phenotype improved metabolic profile young (14 weeks age) genetically Zucker fa/fa rats. Heart weight (HW) HW/tibia length ratio was significantly lower...
We have previously developed a method for estimating elastin content and organization in resistance arteries, where it is minor component. The aim of the present study was to validate against quantitative assay determine relative importance intrinsic elasticity small arteries. Mesenteric third order branches (from 10-day-old, 1- 6-month-old rats) middle cerebral arteries were pressurized. beta-Values calculated from stress-strain relationships used as indicators stiffness. same...
Resistance artery narrowing and stiffening are key elements in the pathogenesis of essential hypertension, but their origin is not completely understood. In mesenteric resistance arteries (MRA) from spontaneously hypertensive rats (SHR), we have shown that inward remodeling associated with abnormal elastic fiber organization, leading to smaller fenestrae internal lamina. Our current aim determine whether this alteration an early event precedes vessel narrowing, or if reorganization SHR...
The vasodilator effect of eriodictyol (5,7,3′,4′ -tetrahydroxyflavanone), isolated previously from the medicinal plant Satureja obovata Lag., was studied in rat thoracic aorta rings. Eriodictyol relaxed a concentration-dependent manner noradrenaline (10-6 M) and KCl (80 mM) induced contractions. relaxant more potent precontracted preparations (IC50 = 6.11 ± 0.2 × 10-5 than those with 2.96 0.1 10-4 M). produced weakly inhibition phasic component by while tonic phase these contractions...
Liver growth factor (LGF), a mitogen for liver cells, reduces fibrosis in rat model of cirrhosis. The present study assesses the possible vascular antifibrotic and antihypertensive effects LGF treatment on spontaneously hypertensive rats (SHR).Six-month-old male SHR normotensive Wistar Kyoto (WKY) were treated with (4.5 microg LGF/rat i.p. twice week 2 weeks). Haemodynamic parameters measured anaesthetized rats. Vascular structure function studied carotid arteries using optical confocal...
Leptin regulates energy expenditure and body weight by acting both on the hypothalamus peripheral targets. Central actions of leptin are enhanced cholecystokinin (CCK). The interaction between CCK makes physiological sense, as rats lacking CCK1 receptors resistant to but not directly infused into brain. We have recently reported that enhances effects increasing entry CNS. aim this work was further characterize effect (10 microg kg(-1)) kinetics well receptor subtype involved in...