A5067992407- Quantum Dots Synthesis And Properties
- Advanced Fluorescence Microscopy Techniques
- Advanced Biosensing Techniques and Applications
- Fullerene Chemistry and Applications
- Platelet Disorders and Treatments
- Force Microscopy Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Chalcogenide Semiconductor Thin Films
- Advanced NMR Techniques and Applications
- Graphene research and applications
- Blood groups and transfusion
- Synthesis and Properties of Aromatic Compounds
- Perovskite Materials and Applications
- Advanced biosensing and bioanalysis techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- Solid-state spectroscopy and crystallography
- Advanced Electron Microscopy Techniques and Applications
- Advanced Chemical Physics Studies
- Cell Image Analysis Techniques
- Atomic and Subatomic Physics Research
- Molecular Junctions and Nanostructures
- Nanocluster Synthesis and Applications
- Analytical Chemistry and Sensors
- Biotin and Related Studies
- Optical Imaging and Spectroscopy Techniques
Utrecht University
2007-2022
Institute of Molecular Biology and Biophysics
2010
University of Amsterdam
2001-2007
Joint Institute for VLBI ERIC
2004
Univé (Netherlands)
2004
Leiden University
1992-2000
University of Twente
1992-1998
Huygens Institute for History and Culture of the Netherlands
1994-1998
Radboud University Nijmegen
1995
Washington University in St. Louis
1992
The room-temperature luminescence of single CdSe/ZnS core−shell quantum dots is investigated by spectrally and temporally resolved confocal microscopy. A large (30 nm) blue shift observed in the emission wavelength during illumination air. In nitrogen, no observed. air ascribed to a 1 nm shrinkage CdSe core photooxidation. Photobleaching occurs about 4 times faster than indicating formation nonradiative recombination centers initial light output higher which may be due reduction defect state...
Fluorescent nanoparticles have been synthesized with a metal core, silica-spacer shell, and dye-labeled silica shell. Cascade-yellow-labeled, silver-core carboxyfluorescein-labeled, gold-core dye–metal distances of 24–25 nm exhibit fluorescence enhancement factors as high 12.5 6.8, respectively. particles distance ∼15 show significant quenching.
The temperature-sensitive luminescence of nanoparticles enables their application as remote thermometers. size these nanothermometers makes them ideal to map temperatures with a high spatial resolution. However, resolution mapping >373 K has remained challenging. Here, we realize nanothermometry resolutions at elevated using chemically stable upconversion and confocal microscopy. We test this method on microelectromechanical heater study the temperature homogeneity. Our experiments reveal...
Fluorescent proteins have become an invaluable tool in cell biology. The green fluorescent protein variant EGFP is especially widely applied. Use of proteins, including EGFP, however can be hindered by inefficient folding, resulting aggregation and reduced fluorescence. This profound prokaryotic cells. Furthermore, EBFP, a blue rarely used because its dim fluorescence fast photobleaching. Thus, efforts to improve properties such as brightness, photostability are important. Strongly enhanced...
The study of lipoproteins, natural nanoparticles comprised lipids and apolipoproteins that transport fats throughout the body, is key importance to better understand, treat, prevent cardiovascular disease. In current study, we have developed a lipoprotein-based nanoparticle consists quantum dot (QD) core Cy5.5 labeled lipidic coating. methodology allows judicious tuning QD/Cy5.5 ratio, which enabled us optimize Förster resonance energy transfer (FRET) between QD Cy5.5-labeled This phenomenon...
We report the synthesis of ultranarrow (Zn,Cd)Te/CdSe colloidal heteronanowires, using ZnTe magic size clusters as seeds. The wire formation starts with a partial Zn for Cd cation exchange, followed by self-organization into segmented heteronanowires. Further growth occurs inclusion CdSe. heteronanowires emit in 530 to 760 nm range high quantum yields. electron-hole overlap decreases increasing CdSe volume fraction, allowing optical properties be controlled adjusting heteronanowire composition.
In this work, gold nanoparticles coated with a fluorescently labelled (rhodamine B) silica shell are presented as fiducial markers for correlative light and electron microscopy (CLEM). The synthesis of the particles is optimized to obtain homogeneous, spherical core-shell arbitrary size. Next, different fluorophore densities characterized determine under which conditions bright (photo)stable can be obtained. 2 3D CLEM examples where used correlation. 2D example, fiducials added cryosection...
Background Storing platelets for transfusion at room temperature increases the risk of microbial infection and decreases platelet functionality, leading to out-date discard rates up 20%. Cold storage may be a better alternative, but this treatment leads rapid clearance after transfusion, initiated by changes in glycoprotein Ibα, receptor von Willebrand factor.Design Methods: We examined change Ibα distribution using Förster resonance energy transfer time-gated fluorescence lifetime imaging...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLowest Excited Singlet State of C60: A Vibronic Analysis the FluorescenceD. J. van den Heuvel, G. B. Berg, E. Groenen, Schmidt, I. Holleman, and MeijerCite this: Phys. Chem. 1995, 99, 30, 11644–11649Publication Date (Print):July 1, 1995Publication History Published online1 May 2002Published inissue 1 July 1995https://pubs.acs.org/doi/10.1021/j100030a004https://doi.org/10.1021/j100030a004research-articleACS PublicationsRequest reuse...
Initial platelet arrest at the exposed arterial vessel wall is mediated through glycoprotein Ibα binding to A1 domain of von Willebrand factor. This interaction occurs sites elevated shear force, and strengthens upon increasing hydrodynamic drag. The increased requires shear-dependent exposure factor domain, but contribution remains ill defined. We have previously found that forms clusters cooling hypothesized such a property enhances with under physiological conditions. analyzed...
Biophysical imaging tools exploit several properties of fluorescence to map cellular biochemistry. However, the engineering a cost-effective and user-friendly detection system for sensing diverse is difficult challenge. Here, we present novel architecture spectrograph that permits integrated characterization excitation, emission anisotropy spectra in quantitative efficient manner. This platform achieves excellent versatility use at comparatively low costs. We demonstrate optical design with...
Förster resonance energy transfer (FRET) using colloidal semiconductor quantum dots (QDs) and dyes is of importance in a wide range biological biophysical studies. Here, we report study on FRET between CuInS2/ZnS QDs dark quencher dye molecules (IRDye QC-1). Oleate-capped with photoluminescence yields (PLQYs) 55 ± 4% are transferred into water by two types multifunctional polymer ligands combining imidazole groups specific moieties amine or methoxy as the terminal sites. The resulting...
In this paper a versatile, high sensitivity spectrograph is presented for use in fluorescence microscopy. The achieved by using prism the dispersion combination with state-of-the-art back illuminated charge-coupled device (CCD) camera. spectrograph, including CCD camera, has detection efficiency of 0.77 ± 0.05 at 633 nm. Full emission spectra 1–5 nm spectral resolution can be recorded maximum rate 800 per second. Two applications are shown, which fiber-coupled to commercial confocal laser...
Electron-nuclear double resonance spectra at 95 GHz and electron-spin-echo envelope-modulation 9.5 are reported for a frozen solution of fully C13 enriched C60 molecules in their photoexcited triplet state. Analysis these data reveals that the wave function is largely localized on part molecule. Upon excitation, molecule adopts structure D5d symmetry with four inequivalent carbon atoms. The equator atoms each carry 3.8% spin density next to 1.1%, together accounting 98% total density. These...