A5051997408- DNA and Nucleic Acid Chemistry
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Cytokine Signaling Pathways and Interactions
- Lymphoma Diagnosis and Treatment
- Advanced biosensing and bioanalysis techniques
- Immune Cell Function and Interaction
- Chemical Synthesis and Analysis
- Microtubule and mitosis dynamics
- Protein Structure and Dynamics
- Biochemical and Molecular Research
- Bacteriophages and microbial interactions
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Enzyme Structure and Function
- Acute Myeloid Leukemia Research
- Radiopharmaceutical Chemistry and Applications
- Cutaneous lymphoproliferative disorders research
- Photosynthetic Processes and Mechanisms
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
University of Wisconsin–Madison
2009-2020
University of Wisconsin Carbone Cancer Center
2016-2018
Significance We demonstrate that STAT3 is a critical transcriptional regulator of the activated B cell–like subtype diffuse large cell lymphoma (ABC DLBCL), most common, aggressive, non-Hodgkin lymphoma. By genome-wide assessment, we have identified target genes STAT3. Gene regulation by in ABC DLBCL accentuates survival signaling pathways while dampening lethal type I interferon pathway. Knowledge these STAT3-regulated has led to our demonstration small-molecule inhibitor JAK1-STAT3 pathway...
Significance Autocrine cytokine signaling in cancer can activate members of the Janus kinase (JAK) family, which are generally thought to act by phosphorylating STAT family transcription factors. We report here that JAK1 mediates autocrine IL-6 and IL-10 activated B-cell–like (ABC) diffuse large B-cell lymphoma (DLBCL) a noncanonical epigenetic regulatory mechanism involving phosphorylation histone H3 on tyrosine 41. have identified target genes ABC DLBCL this mechanism. Knowledge these...
Differences in kinetics of transcription initiation by RNA polymerase (RNAP) at different promoters tailor the pattern gene expression to cellular needs. After initial binding, large conformational changes occur promoter DNA and RNAP form initiation-capable complexes. To understand mechanism regulation initiation, nature sequence these must be determined. Escherichia coli uses binding free energy unwind separate 13 base pairs λPR unstable open intermediate I2, which rapidly converts much...
FRET (fluorescence resonance energy transfer) between far-upstream (−100) and downstream (+14) cyanine dyes (Cy3, Cy5) showed extensive bending wrapping of λPR promoter DNA on Escherichia coli RNA polymerase (RNAP) in closed open complexes (CC OC, respectively). Here we determine the kinetics mechanism by formation RNAP contacts with −100 +14 single-dye protein-induced fluorescence enhancement (PIFE). PIFE exhibit two phases: rapidly reversible steps forming a CC ensemble ({CC}) four...
Abstract Activated B-cell-like diffuse large B-cell lymphoma (ABC DLBCL) is characterized by increased expression and activator of signal transducer transcription 3 (STAT3). ABC DLBCL cells require STAT3 for growth in culture. In cells, eosinophils perhaps all four variant mRNAs (Sα, ΔSα, Sβ ΔSβ) are present as a result two alternative splicing events, one that results the inclusion 55-residue C-terminal transactivation domain (α) or truncated with 7 unique residues (β) second includes (S)...
Centrosomes and spindle pole bodies (SPBs) are membraneless organelles whose duplication assembly is necessary for bipolar mitotic formation. The structural organization functional roles of major proteins in these can provide critical insights into cell division control. Spc42, a phosphoregulated protein with an N-terminal dimeric coiled-coil (DCC), assembles hexameric array at the budding yeast SPB core, where it functions as scaffold assembly. Here, we present vitro vivo data to elucidate...
Abstract FRET (fluorescence energy transfer) between far-upstream (−100) and downstream (+14) cyanine dyes showed extensive bending/wrapping of λP R promoter DNA on E. coli RNA polymerase (RNAP) in closed open complexes (CC, OC). Here we determine the kinetics mechanism by formation RNAP contacts with −100 +14 single-dye fluorescence enhancements (PIFE). FRET/PIFE exhibit two phases: rapidly-reversible steps forming a CC ensemble ({CC}c four intermediates (initial (RP C ), early (I 1E mid-(I...