A5040416730- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- DNA and Nucleic Acid Chemistry
- Microtubule and mitosis dynamics
- Gene Regulatory Network Analysis
- Artificial Intelligence in Healthcare and Education
- Bacteriophages and microbial interactions
- Photosynthetic Processes and Mechanisms
- Biotin and Related Studies
- Biomedical and Engineering Education
- Machine Learning in Materials Science
- Genetics, Bioinformatics, and Biomedical Research
- Protein Structure and Dynamics
- Nuclear Structure and Function
- Poxvirus research and outbreaks
- Scientific Computing and Data Management
- E-Learning and Knowledge Management
- Genomics and Chromatin Dynamics
- Online Learning and Analytics
- Virus-based gene therapy research
- Plant nutrient uptake and metabolism
- Academic Writing and Publishing
- Topic Modeling
- CRISPR and Genetic Engineering
- Protein Degradation and Inhibitors
Winona State University
2021-2025
University of Minnesota
2017-2018
University of Wisconsin–Madison
2012-2017
Twin Cities Orthopedics
2017
AI technologies are rapidly pervading many areas of our world. AI-driven text generators such as ChatGPT at the forefront this due to their simplicity and accessibility. Their influence on higher education is already being observed, perceptions among faculty students vary widely. We have undertaken a cross-curriculum study ChatGPT's ability generate laboratory reports. AI-generated reports from general, organic, analytical, physical, inorganic, biochemistry courses were graded if they...
Large language models (LLMs) such as ChatGPT have recently been challenging traditional of higher education. Given the growing use these tools for writing, research, and content retrieval tasks, it is imperative that both students faculty understand their capabilities shortcomings. Here we describe assignments in which lower- upper-division evaluated chemistry writing samples generated revised by version 3.5 used this program revision other tasks. General Chemistry who AI-generated showed...
To investigate roles of the discriminator and open complex (OC) lifetime in transcription initiation by Escherichia coli RNA polymerase (RNAP; α2ββ'ωσ70), we compare productive abortive rates, short distributions, OC for λPR T7A1 promoters variants with exchanged discriminators, all same transcribed region. The determines these promoters. Permanganate reactivity thymines reveals that strand backbones regions long-lived λPR-discriminator OCs are much more tightly held than shorter-lived...
Many eukaryotic protein kinases are activated by phosphorylation on a specific conserved residue in the regulatory activation loop, post-translational modification thought to stabilize active DFG-In state of catalytic domain. Here we use battery spectroscopic methods that track different elements kinase domain show ~100 fold mitotic Aurora A (AurA) occurs without population shift from DFG-Out state, and loop remains highly dynamic. Instead, molecular dynamics simulations electron...
Significance Many drugs trigger changes to the structure of their target receptor upon binding. These conformational effects are thought be an essential part molecular recognition but have proven challenging quantify. Using a high-throughput method for tracking structural in protein kinase solution, we discovered that many clinically important cancer substantial Aurora A, and these systematically account ability differentiate between different biochemical forms A. The results provide insight...
Differences in kinetics of transcription initiation by RNA polymerase (RNAP) at different promoters tailor the pattern gene expression to cellular needs. After initial binding, large conformational changes occur promoter DNA and RNAP form initiation-capable complexes. To understand mechanism regulation initiation, nature sequence these must be determined. Escherichia coli uses binding free energy unwind separate 13 base pairs λPR unstable open intermediate I2, which rapidly converts much...
Large language models (LLMs) such as ChatGPT are being used for outlining, writing assistance, and higher-level research tasks in many areas of science. The purpose the work described here was to assess version 3.5's ability help upper-level Analytical Inorganic Chemistry students find methods green modifications use laboratory proposals. Some found that provided a helpful starting point, but it mostly gave vague erroneous responses when asked provide these areas, particularly chemistry. Few...
Artificial intelligence (AI) tools are spurring rapid developments in academia, industry, and health care, free generative AI like ChatGPT can now perform some basic writing summary tasks. These technologies will likely continue to gain power capability, today's Chemistry majors may work at point settings augmented by AI. Instructors must adapt the availability of prepare our students for this evolving reality. In spring 2023, define a baseline chemistry education, group faculty from...
Abstract Many eukaryotic protein kinases are activated by phosphorylation on a specific conserved residue in the regulatory activation loop, post-translational modification thought to stabilize active DFG-In state of catalytic domain. Here we use battery spectroscopic methods that track different elements kinase domain show ~100-fold mitotic Aurora A (AurA) occurs without population shift state, and loop remains highly dynamic. Instead, molecular dynamics simulations electron paramagnetic...
E. coli RNA polymerase (RNAP) is a large molecular machine (subunits: αββ′ω and specificity factor σ 70 ) with many moving parts that binds to opens duplex promoter DNA initiate transcription. These events are set in motion by initial interactions between the −35 −10 elements of promoter, flexibly tethered α‐CTDs UP element promoter. Binding free energy drives series conformational changes culminate opening 13 base pairs region start site, forming an unstable open complex, which stabilized...
Vaccinia-related kinase 1 (VRK1) is a serine/threonine that plays variety of roles in transcription regulation and the cell cycle. Its substrates include histones, p53, coilin. The activity VRK1 protein largely controlled by its C-terminal tail, which interacts with enzyme active site. Several rare point mutations (including L195V, R89Q, Y213H) are associated neurodegenerative disorders, questions remain as to how these affect VRK1's intrinsic stability activity. For this vitro study, were...
Most of the >500 protein kinase domains encoded by human genome function in signal transduction pathways controlling aspects cell life cycle. Errors signaling due to overexpression or mutation kinases can result diseases such as cancer, and many disease-linked mutations have been identified. A mutation's pathogenicity may stem from effects on fundamental features stability, ligand binding, catalysis, and/or regulation. In this multi-week project developed for first semester Biochemistry lab,...