The modulation of the gut microbiota was recently deemed one mechanisms responsible for excellent outcomes bariatric surgery. However, to date, only few studies have assessed this, and they high heterogeneity. In present study, next-generation 16S ribosomal DNA amplicon sequencing used characterize healthy volunteers, as well patients prior after sleeve gastrectomy (SG) or Roux-en-Y gastric bypass (RYGB). Significant differences in α diversity, β diversity species were identified between...

To investigate the role of nitric oxide (NO) in acute lung inflammation and injury secondary to necrotizing pancreatitis (ANP), 5% sodium taurocholate was retrogradely injected into biliopancreatic duct rats ANP model. These were given L-Arginine (L-Arg, 100 mg/kg), L-NAME (10 or their combination by intraperitoneal injection 30 min prior induction. At 1, 3, 6, 12 hours after induction, NO production, inducible synthase (iNOS) expression measured. Lung histopathological changes,...

The aim of the present study was to identify potential therapeutic targets for colorectal cancer (CRC). gene expression profile GSE32323, containing 34 samples, including 17 specimens CRC tissues and paired normal from patients, downloaded Gene Expression Omnibus database. Following data preprocessing using Affy preprocessCore packages, differentially-expressed genes (DEGs) between two types samples were identified with Linear Models Microarray Analysis package. Next, functional pathway...

y e f c t s o RNA interference (RNAi) on expression of matrix metalloproteinase-2 (MMP-2 ) gene and invasiveness adhesion human pancreatic cancer cell line, BxPC-3. METHODS:RNAi was performed using the vector (pGPU6)-based small (siRNA) plasmid silence system to specifically knock down MMP-2 in BxPC-3.Four groups different specific target sequence coding region one non-specific were chosen construct four experimental siRNA plasmids pGPU6-1, pGPU6-2, pGPU6-3 pGPU6-4, negative control pGPU6...

Radixin, encoded by a gene on chromosome 11, plays important roles in cell motility, invasion and tumor progression. However, its function pancreatic cancer remains elusive. In this study, radixin expression was suppressed with lentivirus-mediated short-hairpin RNA (shRNA) method. We found that shRNA caused down-regulation of PANC-1 cells, associated inhibition proliferation, survival, adhesion invasive potential vitro. When radixin-silenced cells were implanted nude mice, growth microvessel...

The long-term outcome of laparoscopic surgery for geriatric patients with colorectal cancer remains unclear due to decreased functional reserves and increased medical comorbidities. A meta‑analysis was performed in the present study compare between laparoscopy laparotomy. Randomized controlled trials comparative studies regarding vs. open elderly were searched Pubmed, Embase, Cochrane library inception April 20, 2017. methodological quality cohort evaluated using Newcastle‑Ottawa Scale....

Background and Objectives K-ras (Kirsten-ras) point mutation (PM) in codon 12 are suggested to be significantly associated with the tumorigenesis of pancreatic cancer. The incidences PMs human cancer reported different between Europeans Japanese. present study was designed compare profile ras-p21 expression primary invasive ductal carcinoma (IDC) pancreas Japanese Chinese. Methods specimens included 51 34 Chinese patients IDC pancreas. were tested by allele specific oligonucleotide dot blot...

This study aimed to probe the correlation of long-term exposure traffic noise with risk type 2 diabetes mellitus (T2DM).

Pancreatic cancer is one of the most aggressive tumors with a dismal prognosis. The membrane cytoskeletal crosslinker Ezrin participates in several functions including cell proliferation, adhesion, motility and survival. There increasing evidence that overexpressed vast majority malignant regulates tumor progression. However, its roles pancreatic remain elusive.Three pairs specific siRNAs were designed synthetized screened to determine efficient for construction hairpin RNA plasmid targeting...

Chemotherapy is commonly used for the treatment of breast cancer. However, resistance to chemotherapeutic agents, often mediated by multidrug (MDR) mechanisms, a common occurrence. The present study examined expression several MDR‑related proteins (MRPs) in invasive ductal carcinoma (IDC) breast, and assessed their association with clinicopathological variables prognostic significance. In addition, immunohistochemistry was measure MRP, p‑glycoprotein (P‑gp), topoisomerase 2α (Topo2α),...

Previous studies reported different frequencies of p53 expression between Japanese and Americans or Europeans. The present study was designed to clarify whether there is a significant difference in its clinical implications Chinese patients with primary invasive ductal carcinoma (IDC) the pancreas. studied 39 47 patients, immunostaining SAB method performed using anti-p53 monoclonal antibody (DO-1) formalin-fixed paraffin-embedded specimens. Clinical data were analyzed according...

The aim of this study was to investigate the effects ileal interposition (IT) on glucose and insulin resistance (IR) in type 2 diabetic mellitus (T2DM), role T-cell factor 7-like (TCF7L2), formerly known as TCF4, downregulation hyperglycemia following IT. Goto-Kakizaki (GK) rats subjected IT surgery (GK-IT group), GK sham (GK-Sham group) Wistar (WS) (WS-Sham were investigated study. Fasting plasma glucose, body weight, food intake per 1 kg a homeostasis model assessment (HOMA-IR) measured...

The present study suggests that Ki-ras point mutations may play an important role in the early stages of tumorigenesis and a double mutation has stronger detrimental effect than single on survival after pancreatectomy.Previous studies have suggested ras gene codon 12 pancreatic cancer, but their clinicopathological significance is still unclear. was designed to assess mutations, p21 expression malignant benign diseases pancreas.Oligonucleotide dot-blot hybridization for immunohistochemical...