Proton extrusion by roots of intact sunflower plants (Helianthus annuus L.) was studied in nutrient solutions or agar media with a pH indicator. enhanced either iron deficiency, addition fusicoccin, single salt ammonium potassium salts. The three types proton differ both localization along the and capacity. From their sensitivity to ATPase inhibitors it seems justified characterize them as pumps driven plasma membrane APTases.Enhanced induced preferential cation uptake from (NH(4))(2)SO(4)...

We present a detailed description of the fabrication and operation at room temperature novel type tunnel displacement transducer. Instead feedback system it relies on large reduction factor assuring an inherently stable device. Stability measurements in regime infer electrode stability within 3 pm 1 kHz bandwidth. In contact conductance takes discrete number values when constriction is reduced atom by atom. This reflects conduction through channels.

The adsorption of bovine serum albumin (BSA) on surfaces dental enamel and materials was investigated by scanning force spectroscopy. This method provides adhesion forces which can be measured as a function contact time between protein surface, pH, wettability, isoelectric point the surface. Whereas chosen ceramic composite resemble very well natural enamel, much stronger found for more hydrophobic surfaces, that is, gold, titanium, poly(methyl methacrylate) (PMMA), poly(tetrafluoroethylene)...

Abstract Bacterial nanocellulose (BNC) is a promising material for the use in medical implants. BNC does not induce unwanted reactions vivo , long term stable and possesses unique mechanical properties. However, to make most of these features, must be carefully processed. Details cultivation post-synthetic methods offer various ways control properties BNC. The focus this work put on drying Different unconstrained (climate chamber at 23°C, oven 100°C, freeze-drying) constrained under...

Abstract Two model proteins, bovine serum albumin (BSA) and lysozyme (LSZ), were used to study adsorption behavior on dental titanium by time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS). Protein films prepared as single component layers mixtures of the two proteins. Additionally, consecutive both proteins was investigated. The obtained data analyzed means principal analysis (PCA). Dental samples with a layer consisting simply one protein could be separated from each other selecting...

Abstract Cerium (IV) initiated “graft‐from” polymerization reactions were employed to convert M‐PVA magnetic particles into polyacrylic acid‐fimbriated cation exchange supports displaying ultra‐high binding capacity for basic target proteins. The modifications, which performed at 25 mg and 2.5 g scales, delivered maximum capacities ( Q max ) hen egg white lysozyme in excess of 320 −1 , combined with sub‐micromolar dissociation constants (0.45–0.69 µm) “tightness binding” values greater than...

Abstract The loosely and firmly adsorbed amount of bovine serum albumin (BSA) on dental titanium gold was investigated by bicinchoninic acid assay (BCA assay). This method does not require special properties the sample like a high reflectivity or conductivity turns BCA into valuable tool to investigate widespread spectrum different substrate materials, e.g., natural enamel. experiments in this work showed that it is possible distinguish between bound protein with assay. On here samples BSA...

Abstract Wet and dry etching methods were used to physically modify the surface of borosilicate glass for further measurements in roughness‐induced protein adsorption. The parameters determine roughness while other are not significantly changed. To exclude a chemical alteration surface, isoelectric points, contact angles, energies analyzed before after process.

Electrochromic devices (ECDs) containing iron-based metallo-supramolecular polymers (Fe-MEPE) and Prussian blue (PB) as active electrode materials, a polymer electrolyte flexible ultra-thin indium tin oxide (ITO) glass transparent conductive substrate are demonstrated here. The electrochromic Fe-MEPE PB thin films were deposited on ITO by means of dip coating electrodeposition, respectively, characterized optically (spectro-)electrochemically. By applying low voltage 2.5 V/−0.5 V, the...

Current purification of the glycoprotein equine chorionic gonadotropin (eCG) from horse serum includes consecutive precipitation steps beginning with metaphosphoric acid pH fractionation, two ethanol steps, and dialysis followed by a numerous fixed‐bed chromatography up to specific activity required. A promising procedure for more economic represents simplified process requiring only onethird solvent, usage magnetic ion exchange adsorbents employed together newly designed ‘rotor‐stator’ type...

Abstract The glycoprotein hormone equine chorionic gonadotropin (eCG) is a commercial product used in animal breeding as well veterinary medicine. current state of the art for purification eCG from serum pH fractionation with metaphosphoric acid, two ethanol precipitation steps dialysis followed by fixed‐bed chromatography. Two simplified processes, including use magnetic microsorbents have been developed. processes reduce or even omit organic solvents and required solid‐liquid separation...

Abstract For a better understanding of biofilm establishment, adhesion forces proteins on immobilized protein layers were investigated by scanning force spectroscopy (SFS). this purpose the experimental setup to immobilize surface is important. The influence method chosen was examined and afterwards effect variations in pH contact time measured protein–protein interaction analyzed. adsorption bovine serum albumin (BSA) BSA‐coated silicon used here as model system. To coat substrate, free...

Abstract The application of scanning force microscopy (SFM) in biology has increased during the last years because it allows measurements without pretreatment and under physiological conditions. In addition to pure imaging is possible use SFM as a sensor detect forces small piconewtons. Such are relevant interactions between bacterial cells surfaces or biofilms on surfaces. Furthermore, so‐called mapping modes were recently developed combinations spectroscopy modes. Especially field biofilm...

Abstract Glass is one of the most important materials in medical packaging. Protein adsorption at glass walls leads to loss active substance vials and thus high costs for pharmaceutical industry, particular case expensive drugs. Thus more knowledge about basic mechanisms involved parameters protein interaction with surfaces desirable. In this context, bovine serum albumin (BSA), fibrinogen (FIB), lysozyme (LYS) on typical (borosilicate glass, quartz glass) was investigated by dynamic contact...

Abstract Bevor sich auf einer festen Oberfläche ein Biofilm bildet, adsorbieren in der Regel Proteine. Ein solches Adsorbat zu charakterisieren, erfordert die Kombination mehrerer oberflächenanalytischer Methoden, alle ihre spezifischen Grenzen haben. Oft gelingt es nur kleinen Schritten und über Modellsysteme, Proteinadsorbat realen beschreiben.