A5007109205- RNA modifications and cancer
- RNA Research and Splicing
- Pharmaceutical and Antibiotic Environmental Impacts
- Cancer, Hypoxia, and Metabolism
- Click Chemistry and Applications
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Environmental Toxicology and Ecotoxicology
- Metal-Catalyzed Oxygenation Mechanisms
- Protein purification and stability
- HIV/AIDS drug development and treatment
- Digestive system and related health
- Infant Nutrition and Health
- DNA Repair Mechanisms
- Sirtuins and Resveratrol in Medicine
- Birth, Development, and Health
- Chemical Reactions and Isotopes
- Aquatic Ecosystems and Phytoplankton Dynamics
- Advanced Proteomics Techniques and Applications
- Synthesis and biological activity
- Biochemical and Molecular Research
- Adipose Tissue and Metabolism
- Effects and risks of endocrine disrupting chemicals
- Autophagy in Disease and Therapy
- Animal Genetics and Reproduction
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2015-2024
Polish Academy of Sciences
2019
Abstract The nine identified human homologues of E . coli AlkB 2-oxoglutarate (2OG) and Fe(II)-dependent dioxygenase, ALKBH1-8 FTO, display different substrate specificities diverse biological functions. Here we discovered the combined overexpression members ALKBH family in head neck squamous cell carcinomas (HNSCC). We found direct correlation ALKBH3 FTO expression with primary HNSCC tumor size. observed unidentified thus far cytoplasmic localization ALKBH2 5 HNSCC, suggesting abnormal...
The family of AlkB homolog (ALKBH) proteins, the homologs Escherichia coli 2-oxoglutarate (2OG), and Fe(II)-dependent dioxygenase are involved in a number important regulatory processes eukaryotic cells including repair alkylation lesions DNA, RNA, nucleoprotein complexes. There nine human thirteen Arabidopsis thaliana ALKBH proteins described, which exhibit diversified functions. Among them, ALKBH5 FaT mass Obesity-associated (FTO) protein ALKBH9B ALKBH10B have been recognized as N6...
The waste of commonly used medicines is known to contaminate freshwater ecosystems. Pharmaceuticals can be toxic, mutagenic, or modifying organisms even at low concentrations if consider their permanent presence in the environment. Chemotherapeutics treat cancer, and particular alkylating agents, contribute significantly this form pollution, latter introducing cytotoxic and/or mutagenic lesions DNA RNA which disruptive cells. aim present study was investigate influence anticancer agent...
The intestine of intrauterine growth retarded (IUGR) neonates showed different morphology compared to born with normal body weight (NBW). aim the present study was investigate ultrastructure and proteomic profile gut epithelium in IUGR pig special attention digestive absorptive function. Intestine tissue samples were investigated 7-day-old NBW littermate piglets using histometry, immunofluorescence, scanning electron microscopy (SEM), mass spectrometry analysis. have shown reduced mucosa...
Background: Stilbenes, 1,2-diphenylethen derivatives, including resveratrol and combretastatins, show anticancer features especially against tumor angiogenesis. Fosbretabulin, CA-4, in combination with carboplatin, is the last stages of clinical tests as an inhibitor thyroid cancer. The mode action these compounds involves suppression angiogenesis through interfering tubulin (de)polymerization. Objective: We have previously synthesized five E-2-hydroxystilbenes seven dibenzo [b,f]oxepins Z...
In the present study, synthesis and cytotoxic effect of six stilbenes three oxepine derivatives against two cancerous - HeLa U87, normal EUFA30 HEK293 cell lines has been reported. The results assay flow cytometry analysis revealed that compounds 9-nitrobenzo[b]naphtho[1,2-f]oxepine (4), (E)-3,3',4,4',5,5'-hexamethoxystilbene (6) 4-hydroxy-2',4'-dinitrostilbene (8) were most active their interaction with tubulin (crystal structure from PDB) analyzed by computer molecular modeling. Molecular...
Abstract To reveal the sources of obesity and type 2 diabetes (T2D) in humans, animal models, mainly rodents, have been used. Here, we propose a pig model T2D. Weaned piglets were fed high fat/high sugar diet suppling 150% metabolizable energy. Measurements weight gain, blood morphology, glucose plasma levels, cholesterol, triglycerides, as well tolerance (oral test, OGTT) employed to observe T2D development. The histology mass spectrometry analyses made post mortem . Within 6 months,...
Genome-wide association studies in the FTO gene have identified SNPs correlating with obesity and type 2 diabetes. In mice, lack of Fto function leads to intrauterine growth retardation lean phenotype, whereas human it is lethal. The aim this study a pig model was determine localization protein different tissues cell compartments, order investigate potential targets action. To better understand physiological role protein, its expression studied pigs age, metabolic status nutrition, using...
The FTO protein is involved in a wide range of physiological processes, including adipogenesis and osteogenesis. This two-domain belongs to the AlkB family 2-oxoglutarate (2-OG)- Fe(II)-dependent dioxygenases, displaying N6-methyladenosine (N6-meA) demethylase activity. aim study was characterize relationships between structure activity FTO. effect cofactors (Fe2+/Mn2+ 2-OG), Ca2+ that do not bind at catalytic site, concentration on properties expressed either E. coli (ECFTO) or baculovirus...
Salivary gland tumours (SGTs) are a heterogeneous group of benign various origins and pathologies, showing number DNA modifications. Previously, in malignant head neck cancer (HNSCC), we found overexpression ALKBH proteins, the homologs Escherichia coli AlkB 2-oxoglutarate Fe(II) dependent dioxygenase. Moreover, proved connection some these dioxygenases with development. Here, studied expression five dioxygenases: 1, 3, 4, 5, FTO SGTs. Using Western blot analysis, three proteins: ALKBH1, SGT...
1,N6-α-hydroxypropanoadenine (HPA) is an exocyclic DNA adduct of acrolein - environmental pollutant and endocellular oxidative stress product. Escherichia coli AlkB dioxygenase belongs to the superfamily α-ketoglutarate (αKG)- iron-dependent dioxygenases which remove alkyl lesions from bases via mechanism, thereby restoring native structure. Here, we provide in vivo vitro evidence that HPA mutagenic effectively repaired by dioxygenase. generated plasmid caused A → C T transversions and, less...
FTO is an N6-methyladenosine demethylase removing methyl groups from nucleic acids. Several studies indicate the creation of complexes with other proteins. Here, we looked for regulatory proteins recognizing parts dioxygenase region. In Calmodulin (CaM) Target Database, found C-domain potentially binding CaM, and proved this finding experimentally. The interaction was Ca2+-dependent but independent on phosphorylation. We that FTO-CaM essentially influences calcium-binding loops in indicating...