A5038559825- Animal Genetics and Reproduction
- Aquaculture Nutrition and Growth
- CRISPR and Genetic Engineering
- Aquaculture disease management and microbiota
- Aquatic Ecosystems and Biodiversity
- Fish Ecology and Management Studies
- Fish Biology and Ecology Studies
- Bacterial Genetics and Biotechnology
- Viral Infectious Diseases and Gene Expression in Insects
Universidade Nilton Lins
2024
Universidade Federal do Rio Grande
2019-2020
The present study evaluated the stability of Bacillus subtilis strains transformed with a replicative or integrative plasmid (via CRISPR-Cas9) to express recombinant phytase. Both transformation methods did not affect growth B. subtilis, but construct and enzymatic activity was reduced in strain plasmid.
This work aimed to perform the genetic manipulation of native B. cereus #25 strain and evaluate colonization in intestinal tract Litopenaeus vannamei. The expression GFPmut1, absence amylase activity, growth spectinomycin plates confirmed transformation (BC25GM). In shrimp bioassay, its addiction feed showed non-difference for water quality, performance, toxic effects L. vannamei (survival 100%). presence cells expressing GFPmut1 midgut animals was observed, suggesting that genetically...