A5038180990- Monoclonal and Polyclonal Antibodies Research
- Herpesvirus Infections and Treatments
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- interferon and immune responses
- HIV Research and Treatment
- T-cell and B-cell Immunology
- Complement system in diseases
- Blood groups and transfusion
- Antimicrobial Peptides and Activities
- RNA Interference and Gene Delivery
- T-cell and Retrovirus Studies
- Neonatal Health and Biochemistry
- CAR-T cell therapy research
- Cancer Cells and Metastasis
- Advanced Fluorescence Microscopy Techniques
- Poxvirus research and outbreaks
- Lung Cancer Research Studies
- IL-33, ST2, and ILC Pathways
- Virus-based gene therapy research
- Fungal Infections and Studies
- Immunodeficiency and Autoimmune Disorders
- Hepatitis B Virus Studies
Bühlmann (Switzerland)
2024
Luxembourg Institute of Health
2016-2024
University of Luxembourg
2019-2024
University of California, Irvine
2012-2016
Georg Speyer Haus
2001-2010
Université de Reims Champagne-Ardenne
1997-2007
Goethe University Frankfurt
2001
Centre Hospitalier Universitaire de Reims
2000
Institut National de la Transfusion Sanguine
1997
Centre National de la Recherche Scientifique
1997
The binding and activation of the discoidin domain receptor 1 by collagen has led to conclusion that proteins from extracellular matrix can directly induce tyrosine kinase-mediated signaling cascades. A region in DDR1 homologous <i>Dictyostelium discoideum</i> protein discoidin-I is also present secreted human RS1. Mutations RS1 cause retinoschisis, a genetic disorder characterized ablation retina. By introducing point mutations into at positions retinoschisis mutations, ligand epitopes were...
Evidence from C57BL/6 mice suggests that CD8(+) T cells, specific to the immunodominant HSV-1 glycoprotein B (gB) H-2(b)-restricted epitope (gB498-505), protect against ocular herpes infection and disease. However, possible role of HLA-restricted gB epitopes, in protective immunity seen HSV-1-seropositive asymptomatic (ASYMP) healthy individuals (who have never had clinical herpes) remains be determined. In this study, we used multiple prediction algorithms identify 10 potential...
Abstract Targeting of the mucosal immune system genital tract with subunit vaccines has failed to induce potent and durable local CD8+ T cell immunity, which is crucial for protection against many sexually transmitted viral pathogens, including HSV type 2 (HSV-2), causes herpes. In this study, we aimed investigate potential a novel lipopeptide/adenovirus 5 (Lipo/rAdv5) prime/boost vaccine induction immunity protect female from The lipopeptide rAdv5 express immunodominant HSV-2 epitope...
We recently found that the herpes simplex virus-1 (HSV-1) latency-associated transcript (LAT) results in exhaustion of virus-specific CD8⁺ T cells latently-infected trigeminal ganglia (TG). In this study we sought to determine if impairment may involve LAT directly and/or indirectly interfering with DC maturation. a small number HSV-1 antigen-positive DCs are present TG CD11c/eYFP mice; however, does not imply these acutely or latently infected. Some adjacent DCs, suggesting possible...
ABSTRACT Persistent pathogens, such as herpes simplex virus 1 (HSV-1), have evolved a variety of immune evasion strategies to avoid being detected and destroyed by the host's system. A dynamic cross talk appears occur between HSV-1 latency-associated transcript (LAT), only viral gene that is abundantly transcribed during latency, CD8 + T cells reside in latently infected human rabbit trigeminal ganglia (TG). The reactivation phenotype TG are with wild-type or LAT-rescued mutant (i.e., LAT...
Considering the limited success of recent herpes clinical vaccine trial [1], new strategies are needed. Infections with simplex virus type 1 and 2 (HSV-1 & HSV-2) in majority men women usually asymptomatic results lifelong viral latency neurons sensory ganglia (SG). However, a minority HSV spontaneous reactivation can cause recurrent disease (i.e., symptomatic individuals). Our findings show that T cells from (i.e. those without recurrences, respectively) recognize different epitopes. This...
Current immunofluorescence protocols are limited as they do not provide reliable antibody staining within large tissue volumes (mm3) and cannot localise quantify multiple antigens or cell populations in the same at high resolution. To address this limitation, we have developed an approach to three-dimensionally visualise resolution (<1 µm) with antigen labelling, for volumetric quantitative analysis. This is made possible through computer reconstruction of serial sectioned sequentially...
Combination antiretroviral therapy (cART) has dramatically extended the life expectancy of people living with HIV-1 and improved their quality life. There is nevertheless no cure for infection since persists in viral reservoirs latently infected CD4+ T cells. cART does not eradicate or restore cytotoxic natural killer (NK) cells which are reduced by infection, express checkpoint inhibitors NKG2A KIR2DL upregulated after infection. Cytotoxic NK expressing homing receptor CXCR5 were recently...
CR1 (CD35, the C3b/C4b receptor) is a widely distributed membrane glycoprotein with unique cluster conformation on surface of erythrocytes (E). E responsible for transport immune complexes (IC) to liver and spleen. As cofactor C3b cleavage by factor I, also potent inhibitor C activation inflammation. In some diseases (systemic lupus erythematosus, hemolytic anemia, AIDS, etc.) an acquired low level has been observed, leading impaired clearance IC. The aim this study was design...
A major drawback of therapeutic peptides is their short half-life, which results in the need for multiple applications and high synthesis costs. To overcome this, we established a eukaryotic expression system that allows stable small by multimerisation. By inserting sequence encoding peptide between signal multimerising domain alpha-chain from human C4bp plasma protein, as 5 kDa are secreted multimers transfected cells; this easy purification. As proof principle, show T20-derived HIV-1...
Directing selective complement activation towards tumour cells is an attractive strategy to promote their elimination. In the present work, we have generated heteromultimeric immunoconjugates that selectively activate alternative pathway (AP) on cells. We used C4b‐binding protein C‐terminal‐α‐/β‐chain scaffold for multimerisation generate displaying (a) a multivalent‐positive regulator of AP, human factor H‐related 4 (FHR4) with; (b) multivalent targeting function directed against erbB2...
The chemokine receptor CXCR4 is involved in the development and migration of stem immune cells but also implicated tumor progression metastasis for a variety cancers. Antagonizing ligand (CXCL12)-induced signaling is, therefore, therapeutic interest. Currently, there are two small-molecule antagonists on market mobilization hematopoietic cells. Other molecules with improved potencies safety profiles being developed different indications, including cancer. Moreover, multiple antagonistic...
ABSTRACT During HIV-1 entry, binding of the viral envelope glycoprotein gp120 to cellular CD4 receptor triggers conformational changes resulting in exposure new epitopes, highly conserved CD4-induced (CD4i) epitopes that are essential for subsequent chemokine CCR5 or CXCR4. Due their functional conservation, CD4i represent attractive targets entry inhibition. The aim present study was select peptide ligands on native dualtropic (R5X4) (Env) glycoproteins by phage display. Using CD4-activated...
Abstract Background Directing selective complement activation towards tumor cells is an attractive strategy to promote their elimination. We have generated Complement-activating Multimeric immunotherapeutic compleXes (CoMiX) that selectively stimulate the alternative pathway using Factor H Related protein 4 (FHR4) or classical pathways triple Fc dimers on HER2-expressing cells. Methods used C4bp C-terminal-α-/β-chain multimerising scaffolds generate CoMiX-FHR4 and CoMiX-Fc with 2 different V...
The density of CR1, the C3b/C4b receptor (CD35), on erythrocytes (E) (CR1/E) is genetically determined. However, broad distribution CR1/E within a given genotype suggests that other genetic elements might contribute to regulation CR1/E. In some pathological conditions, including systemic lupus erythematosus (SLE), AIDS and hemolytic anemia, CR1 deficiency parallels severity disease. When compared healthy individuals, an accelerated decrease in these patients has been demonstrated, but...
Abstract Identification of human “asymptomatic” HSV-1 epitopes that induce CD8+ T cells would help in designing an immunotherapeutic vaccine against herpes. Here, the gB sequence was screened for potential HLA-A*0201 cell using a variety algorithms. The ability these to multi-functional responses compared 10 “symptomatic” vs. individuals. Ten epitope peptides were tested affinity HLA-A*0201. gB441-449 and gB561-569 exhibited high to, significantly stabilized, molecules on surface T2 cells....