A5086737536- Mast cells and histamine
- Sphingolipid Metabolism and Signaling
- Lipid Membrane Structure and Behavior
- Immune Response and Inflammation
- Immune cells in cancer
- Monoclonal and Polyclonal Antibodies Research
- Inflammasome and immune disorders
- Receptor Mechanisms and Signaling
- Immune Cell Function and Interaction
- Cellular Mechanics and Interactions
- Click Chemistry and Applications
- Polyamine Metabolism and Applications
- Inflammatory mediators and NSAID effects
- Synthesis and Reactions of Organic Compounds
- Eosinophilic Disorders and Syndromes
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Lysosomal Storage Disorders Research
- Cell Adhesion Molecules Research
- Endoplasmic Reticulum Stress and Disease
- Asthma and respiratory diseases
Washington University in St. Louis
2021-2025
Czech Academy of Sciences, Institute of Molecular Genetics
2015-2021
Itaconate is a unique regulatory metabolite that induced upon Toll-like receptor (TLR) stimulation in myeloid cells. Here, we demonstrate major inflammatory tolerance and cell death phenotypes associated with itaconate production activated macrophages. We show endogenous key regulator of the signal 2 NLR family pyrin domain containing 3 (NLRP3) inflammasome activation after long lipopolysaccharide (LPS) priming, which establishes to late NLRP3 activation. acts synergistically inducible...
Mast cells play an effector role in innate immunity, allergy, and inflammation. Antigen-mediated activation of mast initiates signaling events leading to Ca2+ response the release inflammatory allergic mediators from granules. Diseases associated with deregulated cell functions are hard treat there is increasing demand for new therapeutic strategies. Miltefosine (hexadecylphosphocholine) a candidate treatment cell-driven diseases as it inhibits cells. It has been proposed that miltefosine...
Ethanol has multiple effects on biochemical events in a variety of cell types, including the high-affinity immunoglobulin E receptor (FcεRI) signaling antigen-activated mast cells. However, underlying molecular mechanism remains unknown. To get better understanding effect ethanol FcεRI-mediated we examined short-term treatment with non-toxic concentrations FcεRI mouse bone marrow-derived We found that 15 min exposure to inhibited antigen-induced degranulation, calcium mobilization,...
C-terminal Src kinase (CSK) is a major negative regulator of family tyrosine kinases (SFKs) that play critical roles in immunoreceptor signaling. CSK brought contiguity to the plasma membrane-bound SFKs via binding transmembrane adaptor PAG. The recent finding PAG can function as positive high-affinity IgE receptor (FcεRI)-mediated mast cell signaling suggested and have some non-overlapping regulatory functions activation. To determine FcεRI signaling, we derived bone marrow (BMMCs) with...
Leukotrienes and sphingolipids are critical lipid mediators participating in numerous cellular signal transduction events developing various disorders, such as bronchial hyperactivity leading to asthma. Enzymatic reactions initiating production of these involve 5-lipoxygenase (5-LO)-mediated conversion arachidonic acid leukotrienes serine palmitoyltransferase (SPT)-mediated de novo synthesis sphingolipids. Previous studies have shown that ER membrane protein ORMDL3 inhibits the activity SPT...
Abstract Itaconate is a unique regulatory metabolite that induced upon toll-like receptor (TLR) stimulation in myeloid cells. Here, we demonstrate major inflammatory tolerance and cell death phenotypes associated with itaconate production activated macrophages. We show endogenous key regulator of the signal 2 NLRP3 inflammasome activation after long LPS priming which establishes to late activation. acts synergistically iNOS ability various TLR ligands establish depends on pattern...
Abstract Leukotrienes and sphingolipids are critical lipid mediators participating in cellular signal transduction development of various diseases. Metabolic pathways initiating production these involve 5-lipoxygenase (5-LO)-mediated conversion arachidonic acid to leukotrienes serine palmitoyltransferase (SPT) de novo synthesis sphingolipids. Previous studies showed that endoplasmic reticulum membrane protein ORMDL3 inhibits the activity SPT sphingolipid synthesis. However, role is not...
Abstract Single-nucleotide polymorphism studies have linked the chromosome 17q12-q21 region, where human ORMDL3 gene is localized, to risk of asthma and other inflammatory diseases. Although mast cells are involved in development these diseases, contribution cell physiology unknown. In this study we examined role mouse bone marrow-derived with reduced or enhanced expression. Our data show that antigen-activated cells, expression protein had no effect on degranulation, calcium response,...