Measurement of testicular artery blood flow is used in several species to evaluate reproductive function and scrotal pathology. In dogs there are inconsistent reports about normal post-pubertal no information concerning pre-pubertal dogs. The aim this study was describe regional differences clinically with history tract disease. produced ejaculates throughout the study. all three different regions were imaged monophasic an obvious systolic peak diastole observed on every occasion. highest...

Currently, it has been observed that a considerable segment of the jaguar population is declining mainly because hunting, and destruction fragmentation habitat. Given this scenario, efforts scientific community have concentrated on development conservation strategies, such as formation use somatic sample banks. We aimed to assess effects cryopreservation techniques ear skin [slow freezing (SF) or direct vitrification in cryovials (DVC) solid-surface (SSV)] morphological analysis cell ability...

Neotropical carnivores include a large number of threatened and endangered species. It is critical to develop conservation efforts ensure the sustainability populations in situ ex situ. The highest priorities are protect natural habitats better understand biology rare Conservation also directed toward implementation breeding programs development reproductive biotechnologies which cryopreservation male gametes plays major role. fundamental create semen banks that contribute maintaining...

Due to the global decrease in jaguar population, conservation strategies are essential and development of effective semen cryopreservation protocols would contribute formation germplasm banks. Therefore, objectives were (1) evaluate use TRIS ACP-117c extenders for freezing, (2) describe ultrastructural changes sperm after cryopreservation, (3) binding capacity thawed sperm. Eight ejaculates from five mature individuals collected by electroejaculation, extended or a coconut based-extender...

The puma population is constantly decreasing, and cloning by somatic cell nuclear transfer can be used to conserve the species. One of factors determining success development cloned embryos cycle stage donor cells. We evaluated effects full confluency (~100%), serum starvation (0.5% serum), roscovitine (15 µM) treatments on synchronization in G0/G1 skin-derived fibroblasts flow cytometric analysis. Also, we assessed these methods morphology, viability, apoptosis levels using microscopy...

Abstract Sperm morphometry is the tool that confers objectivity to morphological evaluation by accurately measuring dimensions of gamete and its structures. Thus, aim study was perform a morphometric characterization domestic cat sperm. Therefore, sperm samples were collected from twenty pairs epididymis in TRIS extender at 37ºC. An aliquot sample used make smear with Rose Bengal solution, afterwards, morphology analysed. In morphology, quantified percentage normal cells, changes head,...

Cryopreservation of cats epididymal spermatozoa allows the conservation genetic material and study cryogenic effect applied to gametes other felines. However, this biotechnique still presents variable results, being necessary investigation alternative extenders. Powdered coconut water (ACP-117c) has been efficient in sperm freezing several species cat refrigeration. Therefore, we aimed evaluate stages quality cats' after thawing, using ACP-117c. Epididymides (n = 36) from 18 were processed...

Abstract The establishment of fibroblast lines enables several applications from the formation biobanks for conservation biodiversity to use these cells in physiological and toxicological assays. Considered a species vulnerable extinction, characterization fibroblastic northern tiger cat would contribute its conservation. Therefore, we established characterized fibroblasts derived during extended passage (third, seventh, eleventh passages) cryopreservation with regard morphology, viability,...

Anhydrous preservation is a promising approach for storage of living biomaterials at nonfreezing temperatures. Using the domestic cat model, objectives this study were to characterize changes in histology, DNA integrity, and viability testicular tissues from adult versus prepubertal individuals during microwave-assisted drying. Testes each age group cut into small pieces before reversible membrane permeabilization, exposure trehalose, drying different time periods. In Experiment 1, water...

Heterologous in vitro fertilization (IVF) is an important tool for assessing fertility of endangered mammals such as the jaguar, considering difficult access to females artificial insemination and obtain homologous oocytes. We aimed evaluate jaguar sperm cryopreserved with different extenders, using domestic cat oocytes assess development hybrid embryos. Semen from four captive jaguars was obtained by electroejaculation. Samples were powdered coconut water (ACP-117c) or Tris extender...

The aim was to describe, through scanning electron microscopy (SEM) and transmission (TEM), the ultrastructure of peccaries' fresh frozen-thawed sperm. For that, semen derived from three mature males obtained by electroejaculation evaluated for motility, membrane integrity, functionality, chromatin morphology light microscopy. Samples were frozen using a Tris extender plus egg yolk (20%) glycerol (6%). Then, samples mixed in different sperm pools that processed SEM TEM. Sperm functionality...

Morphological sperm evaluation supported by the morphometry can be used in determination of seminal quality and investigation potential extenders. Although there are studies comparing TRIS ACP extenders, no comparative between them for computerized assisted semen analysis (CASA), viability, membrane functionality parameters cryopreserved canine semen. Hence, we aimed to evaluate effects ACP-106c on post-freezing quality. Five dogs were submitted collection twice with one-week interval. The...

Abstract Populations of gray brocket deer ( Mazama gouazoubira ) are declining; yet, knowledge on the reproductive biology this species remains limited. Therefore, study aimed to describe morphology, viability, membrane integrity, mitochondrial activity, morphometry, micromorphology, and ultrastructure sperm. Three adult male were used in study. Semen collection was performed using electroejaculation. analyzed by evaluating pH, motilities, vigor, mass movement, volume, concentration,...

Resumo: Este estudo foi realizado para determinar o efeito de hidroxitolueno butilado (BHT) sobre a qualidade do sêmen canino congelado e descongelado, utilizando diluidor à base água coco em pó (ACP-106c). Para tanto, foram realizadas quinze coletas provenientes cinco cães. O obtido diluído ACP-106c acrescido glicerol gema ovo. As amostras então transferidas tubos contendo diferentes concentrações BHT (0; 0,5; 1,0 2,0 mM). Em seguida, as envasadas, congeladas armazenadas nitrogênio líquido....

The ear tissue of vulnerable felines, such as the puma, holds potential biological material for creating biobanks. However, skin composition can differ significantly between individuals same species and even sexes, based on different environments. Therefore, identifying morphological similarities across these populations is crucial developing accurate protocols. This study aimed to characterize evaluate structure in both a male female puma using histological techniques. Histomorphometric...