A5088581391- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Skin Protection and Aging
- Animal Genetics and Reproduction
- Plant tissue culture and regeneration
- Wildlife Ecology and Conservation
- Tissue Engineering and Regenerative Medicine
- Meat and Animal Product Quality
- Marine Biology and Environmental Chemistry
- Wound Healing and Treatments
- Pluripotent Stem Cells Research
- Animal Behavior and Reproduction
- Adipose Tissue and Metabolism
- Osteoarthritis Treatment and Mechanisms
- Bee Products Chemical Analysis
- Silk-based biomaterials and applications
- Forensic Entomology and Diptera Studies
- Aquaculture Nutrition and Growth
- Agricultural and Food Sciences
- Corneal Surgery and Treatments
- High Altitude and Hypoxia
- Reproductive biology and impacts on aquatic species
- Renal and related cancers
- Cardiovascular and exercise physiology
- Collagen: Extraction and Characterization
Universidade Federal Rural do Semi-Árido
2020-2024
Universidade Federal de São Paulo
2015
The cryobanks of agouti somatic tissues represent a promising tool for the conservation this species and those that are phylogenetically related endangered. For these purposes, one strategy to guarantee quality samples after warming would be choose appropriate tissue vitrification technique. Therefore, we evaluated effects two different techniques, direct in cryovials (DVC) solid-surface (SSV), on preservation ear derived from agoutis kept scientific center creation. Noncryopreserved were...
Cell lines are valuable tools to safeguard genetic material from species threatened with extinction that is mainly due human action. In this scenario, the puma constitutes a whose population being rapidly reduced in ecosystems it inhabits. For first time, we characterized skin-derived cell and assessed these cells after extended culture (experiment 1) cryopreservation 2). Initially, identified four dermal fibroblast using morphology, ultrastructure, immunofluorescence assays. Moreover,...
Skin cryobanks represent important tools for the conservation of maximum genetic representation a population, especially those with certain degree threat to extinction, such as ocelot. A relevant step towards proper establishment these banks is definition adequate cryopreservation techniques skin.We evaluated effects two different [direct vitrification in cryovials (DVC) and solid-surface (SSV)] preservation ear skin derived from ocelot.For both techniques, we vitrified using Dulbeccos...
Abstract The declining population of the Antillean manatee caused by ecosystem degradation and rising pollution has prompted interest in developing conservation strategies for this species. Given scenario, somatic tissue banks are important tools acquiring knowledge about species, as well obtaining cells biotechnological ecotoxicological applications. Therefore, we aimed to assess effects slow freezing (SF) solid‐surface vitrification (SSV) dermis captive manatees on histology ultrastructure...
Abstract Ex‐situ conservation strategies such as the formation of somatic cell banks are valuable tools for jaguars, whose population has been declining in recent years. Once properly established, these cells can be successfully leveraged future applications. We aimed to assess effects vitro culture and cryopreservation on establishment fibroblasts derived from jaguars. Initially, we identified five dermal fibroblastic lines using morphology immunophenotyping assays; were then subjected two...
Cryopreservation of somatic tissue has been studied as a tool for the knowledge and conservation endangered species, such Antillean manatees. The use vitrification protocols is an important step in establishment biological banks. To decrease damage caused by this technique, reduction concentration cryoprotectants proposed. Therefore, we aimed to evaluate combinations concentrations intracellular tissues derived from Dulbecco's modified Eagle's medium, F-12 composed 10% fetal bovine serum...
ABSTRACT: Skin and cartilage have been the main source for recovery of somatic cells to be used in conservation strategies wild mammals. In this sense, an important step cryopreservation these samples is recognize properties skin cartilage. Thus, knowing that may differ among species aiming contribute establishment cryobanks, study examined differences ear rodents from South America, agouti (Dasyprocta leporina) spix’s yellow-toothed cavy (Galea spixii). Ultrastructural quantitative methods...
Abstract Biological resource banks represent valuable tools for the conservation of species vulnerable to extinction, such as jaguar. Cryobanks skins have potential safeguard rare genotypes, allowing exploitation biological samples in animal multiplication technologies and study genetic variability. Determination most suitable skin regions tissue can help increase efficiency cryobanks storage samples. To this end, we evaluated effects vitrification tissues from ear, caudal, femoral a...
Abstract The biobanks from dermal biopsies represent an interesting strategy for biodiversity conservation. Nevertheless, the morphological and cellular patterns of dermis can be influenced by age sex individual. Therefore, evaluating these factors is forming Antillean manatees. These animals, representatives marine fauna, have had their population reduced, are essential Then, we evaluated effects (3.5 years vs. 3.6–16 23.6 years) (males females) on parameters using histological in vitro...
The ear tissue of vulnerable felines, such as the puma, holds potential biological material for creating biobanks. However, skin composition can differ significantly between individuals same species and even sexes, based on different environments. Therefore, identifying morphological similarities across these populations is crucial developing accurate protocols. This study aimed to characterize evaluate structure in both a male female puma using histological techniques. Histomorphometric...
O objetivo do presente trabalho foi comparar os efeitos da Congelação Lenta (CL) e Vitrificação em Superfície Sólida (VSS) na criopreservação pele auricular apical de gatos domésticos utilizando ferramentas histológicas. Fragmentos obtidos a partir seis (Felis silvestres catus) foram processados destinadas à por CL VSS, uma solução composta Meio Essencial Mínimo Modificado Dulbecco (DMEM) acrescida dimetilsulfóxido (DMSO) 1,5 M ou 3,0 M, para respectivamente, além 0,25 sacarose 10% soro...
Abstract The success of cloning by somatic cell nuclear transfer depends on the efficiency reprogramming, with cycle stage donor playing a crucial role. Therefore, aim was to evaluate three different approaches for synchronization: (i) serum starvation (SS) 1 4 days, (ii) contact inhibition (CI) 3 and (iii) using regulatory inhibitors (dimethyl sulfoxide, cycloheximide, cytochalasin B, or 6-dimethylaminopurine) 2 in terms their effects synchronization G 0 /G phases viability collared peccary...
O objetivo foi avaliar a influência da conservação [refrigeração 5°C e vitrificação] sobre tecidos somáticos de catetos. experimento dividido em duas etapas. Na primeira etapa, fragmentos foram refrigerados por 24 (R-24) 48 h (R-48) comparados com amostras não refrigeradas (C). segunda (RV-24) (RV-48) seguidos vitrificação, apenas vitrificados (V). embora espessura epiderme tenha sido afetada pela refrigeração h, derme reduzida tanto no período quanto h. Além disso, enquanto uma redução...