A5032588708- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Pluripotent Stem Cells Research
- Plant tissue culture and regeneration
- Renal and related cancers
- Wildlife Ecology and Conservation
- Seed Germination and Physiology
- Virus-based gene therapy research
- Tissue Engineering and Regenerative Medicine
- Reproductive Physiology in Livestock
- Skin Protection and Aging
- CRISPR and Genetic Engineering
- Xenotransplantation and immune response
- Genetic and phenotypic traits in livestock
- Bee Products Chemical Analysis
- Wound Healing and Treatments
- Agricultural and Food Sciences
- Marine animal studies overview
- Plant Toxicity and Pharmacological Properties
- Reproductive biology and impacts on aquatic species
- Aquaculture Nutrition and Growth
- Ovarian function and disorders
- Meat and Animal Product Quality
- Milk Quality and Mastitis in Dairy Cows
Universidade Federal Rural do Semi-Árido
2016-2025
Universidade do Estado do Rio Grande do Norte
2023
Animal Production Institute
2022
Universidade Estadual do Ceará
2007-2014
This study aimed to evaluate the effects of β-caryophyllene and its antioxidant potential at different concentrations during in vitro maturation (IVM) bovine oocytes. Immature oocytes were divided into groups: without (control group); groups supplemented with 100 µM cysteamine (CYS), 1 (β1), 5 (β5), 10 (β10) β-caryophyllene. After 24h00 IVM, analyzed for cumulus cell viability expansion, presence first polar body (1PB), metaphase II (MII), cytoplasmic maturation, levels reactive oxygen...
In order to produce transgenic goats with hG-CSF, a total of 24 adult Saanen and 48 undefined breed were used as donors recipients, respectively. Donors estrus-synchronized vaginal sponges superovulated by treatment 200 mg FSH given twice daily in decreasing doses over 3 days starting h before sponge removal. Ovulation was induced injecting 100µg GnRH 36 after The recipients also received an estrus synchronization treatment. mated fertile bucks and, approximately 72 removal, zygotes...
Currently, it has been observed that a considerable segment of the jaguar population is declining mainly because hunting, and destruction fragmentation habitat. Given this scenario, efforts scientific community have concentrated on development conservation strategies, such as formation use somatic sample banks. We aimed to assess effects cryopreservation techniques ear skin [slow freezing (SF) or direct vitrification in cryovials (DVC) solid-surface (SSV)] morphological analysis cell ability...
The objective was to evaluate different permeating cryoprotectants vitrify testicular tissue biopsies from adult collared peccaries. Five pairs of testicles were dissected into fragments (9 mm³) that allocated non-vitrified (control) and vitrified groups using a solid-surface method following exposure (3.0 M dimethyl sulfoxide (DMSO), 3.0 ethylene glycol (EG) or 1.5 DMSO + EG). After warming, samples evaluated for histomorphology, ultrastructure, viability, proliferative capacity potential....
Pyroligneous extract of Jurema preta (Mimosa tenuiflora [Willd.] Poiret) was evaluated for its efficacy as a cutaneous antiseptic in cats (Felis catus) that were subjected to ovariosalpingohysterectomy. For this purpose, 30 without defined breed sterilized and divided into two groups. The first group the positive control, treated with 0.5% chlorhexidine-alcohol solution, second 20% pyroligneous M. tenuiflora. Regardless age sex, all animals had visible healing at similar times. A significant...
To evaluate the applicability of Mimosa tenuiflora and Eucalyptus urograndis pyroligneous acids (PAs) as alternative antiseptics in dairy goats. Cytotoxicity was evaluated vitro using bacteria, well vivo goats, influence PAs on physicochemical parameters fresh milk were examined. The cytotoxicity terms morphology, cell viability metabolic activity goat tegumentary cells. PA M. had results similar to those 2% iodine. For tests, strains Staphylococcus aureus, Escherichia coli Pseudomonas...
The cryobanks of agouti somatic tissues represent a promising tool for the conservation this species and those that are phylogenetically related endangered. For these purposes, one strategy to guarantee quality samples after warming would be choose appropriate tissue vitrification technique. Therefore, we evaluated effects two different techniques, direct in cryovials (DVC) solid-surface (SSV), on preservation ear derived from agoutis kept scientific center creation. Noncryopreserved were...
Cell lines are valuable tools to safeguard genetic material from species threatened with extinction that is mainly due human action. In this scenario, the puma constitutes a whose population being rapidly reduced in ecosystems it inhabits. For first time, we characterized skin-derived cell and assessed these cells after extended culture (experiment 1) cryopreservation 2). Initially, identified four dermal fibroblast using morphology, ultrastructure, immunofluorescence assays. Moreover,...
The production of recombinant human granulocyte-colony stimulating factor (hG-CSF) for therapeutic purposes relies on its expression in selected clones transfected mammalian cells. Alternatively, this protein can be produced by targeted secretion the milk transgenic goats. Thus, aim study was to produce founder goats expressing hG-CSF and propagate a herd. After DNA microinjection vivo-produced pronuclear embryos performed, two founders were obtained (one male one female, named 10 M 12 F,...
Skin cryobanks represent important tools for the conservation of maximum genetic representation a population, especially those with certain degree threat to extinction, such as ocelot. A relevant step towards proper establishment these banks is definition adequate cryopreservation techniques skin.We evaluated effects two different [direct vitrification in cryovials (DVC) and solid-surface (SSV)] preservation ear skin derived from ocelot.For both techniques, we vitrified using Dulbeccos...
The puma population is constantly decreasing, and cloning by somatic cell nuclear transfer can be used to conserve the species. One of factors determining success development cloned embryos cycle stage donor cells. We evaluated effects full confluency (~100%), serum starvation (0.5% serum), roscovitine (15 µM) treatments on synchronization in G0/G1 skin-derived fibroblasts flow cytometric analysis. Also, we assessed these methods morphology, viability, apoptosis levels using microscopy...
This study measured the effects of different freezing techniques and permeating cryoprotectants on preservation testicular tissues from adult red-rumped agoutis. Tissue biopsies (3.0 mm3) five individuals were allocated to experimental groups: control (non-cryopreserved); slow (SF), solid-surface vitrification (SSV), conventional (CV). Each method used dimethyl sulfoxide (DMSO), ethylene glycol (EG), or a DMSO + EG combination. Morphology, viability, mitochondrial activity, proliferative...