A5080861865- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Reproductive Physiology in Livestock
- Sperm and Testicular Function
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Ovarian function and disorders
- Xenotransplantation and immune response
- Genetic and phenotypic traits in livestock
- Molecular Biology Techniques and Applications
- Renal and related cancers
- Research on Leishmaniasis Studies
- Milk Quality and Mastitis in Dairy Cows
- Helminth infection and control
- Tissue Engineering and Regenerative Medicine
- Viral Infectious Diseases and Gene Expression in Insects
- Trypanosoma species research and implications
- RNA Interference and Gene Delivery
- Antifungal resistance and susceptibility
- Pluripotent Stem Cells Research
- Antimicrobial Peptides and Activities
- Pregnancy-related medical research
- Fungal Infections and Studies
- Effects of Environmental Stressors on Livestock
- Venomous Animal Envenomation and Studies
Universidade de Fortaleza
2017-2024
Faculdade Metropolitana de Manaus
2021-2023
Universidade Federal do Rio Grande do Sul
2023
Universidade Estadual do Ceará
2013-2022
Universidade Estadual de Campinas (UNICAMP)
2019
Fundação de Estudo e Pesquisa em Medicina Veterinária e Zootecnia
2017
Universidade Federal do Ceará
2006-2008
O controle do parasitismo por nematódeos gastrintestinais é feito basicamente com a utilização de anti-helmínticos. Falhas no são o primeiro sinal aparecimento resistência anti-helmíntica. A real situação da prevalência anti-helmíntica, em fazendas comerciais criação ovinos e caprinos Brasil, desconhecida. Esse experimento teve como objetivo, estimar ocorrência ao oxfendazol, levamisol ivermectina propriedades caprinos, na região médio baixo Jaguaribe, através teste redução contagem ovos nas...
Visceral Leishmaniasis is an endemic disease in Brazil caused by Leishmania infantum chagasi and its main vector species the sand fly Lutzomyia longipalpis. Epidemiological studies have used conventional PCR techniques to measure rate of infection flies collected field. However, real-time can detect lower parasite burdens, reducing number false negatives improving quantification parasites fly. This study compared genes with various copy numbers quantify L. longipalpis specimens PCR. We mixed...
This study investigated potential mechanisms of azole resistance among Candida albicans from animals, including efflux pump activity, ergosterol content and gene expression. For this purpose, 30 azole-resistant C. strains animals were tested for their antifungal susceptibility, according to document M27-A3, activity by rhodamine 6G test, expression the genes CDR1, CDR2, MDR1, ERG11 RT-qPCR. These resistant at least one derivative. Resistance fluconazole itraconazole was detected in 23 26...
In order to produce transgenic goats with hG-CSF, a total of 24 adult Saanen and 48 undefined breed were used as donors recipients, respectively. Donors estrus-synchronized vaginal sponges superovulated by treatment 200 mg FSH given twice daily in decreasing doses over 3 days starting h before sponge removal. Ovulation was induced injecting 100µg GnRH 36 after The recipients also received an estrus synchronization treatment. mated fertile bucks and, approximately 72 removal, zygotes...
Summary This study aimed to investigate the influence of tetraconazole and malathion, both used in agricultural activities, on resistance fluconazole, itraconazole voriconazole Candida parapsilosis ATCC 22019. The susceptibility tetraconazole, voriconazole, through broth microdilution. Then, 12 independent replicates, were separated exposed four treatment groups, each one containing three replicates: G1: tetraconazole; G2: malathion; G3: fluconazole (positive control); G4: negative control....
The brown brocket deer Mazama gouazoubira is 1 of the 10 recognized Neotropical region. Recently, this species has suffered a population decline due to current threats, mainly poaching and habitat loss. Several studies have shown that some endangered can benefit from interspecies somatic cell nuclear transfer technology through use their cells, such as fibroblasts. Thus, aim study was verify viability effect cryopreservation on fibroblasts after several passages. For purpose, fibroblast...
Among all the transgenic mammalians produced so far, goats have represented an excellent model of transgenesis when considering factors such as market demand for protein, volume milk per lactation and reproductive rate. Various recombinant proteins been obtained from non-transgenic goats, among these, human antithrombin, by was first protein animal origin to be released a drug clinical use in humans. This review reports aspects inherent production bioreactors up expression these their milk.
Abstract Spermadhesins are the major proteins of boar seminal plasma and form a group polypeptides probably involved in reproduction. In previous work, member spermadhesin family from buck plasma, called BSFP, was characterized by mass spectrometry N‐terminal sequencing. The present study aimed to clone characterize BSFP gene investigate its expression along genital tract using real‐time polymerase chain reaction (PCR). cDNAs vesicle, testis, epididymis, bulbourethral gland, ductus deferens...
The production of recombinant human granulocyte-colony stimulating factor (hG-CSF) for therapeutic purposes relies on its expression in selected clones transfected mammalian cells. Alternatively, this protein can be produced by targeted secretion the milk transgenic goats. Thus, aim study was to produce founder goats expressing hG-CSF and propagate a herd. After DNA microinjection vivo-produced pronuclear embryos performed, two founders were obtained (one male one female, named 10 M 12 F,...
The objective of this study was to perform an epidemiological survey determine the areas at risk visceral leishmaniasis through detection and quantification natural infection by Leishmania infantum in Lutzomyia longipalpis. sandflies were captured between February 2009 January 2010, 21 sites four regions Fortaleza municipality. Samples screened for presence DNA Real Time PCR (qPCR), amplification kDNA minicircle sequence. Out 123 pools analyzed sandflies, 45 positive L.infantum, minimum rate...
This paper reviews the technical bases of in vitro embryo production small ruminants with special attention to results obtained by our group Northeastern Brazil. The laparoscopic oocyte recovery hormonally treated live animals indicates a promising future for application this technique genetic improvement program. New molecular biology tools should provide information improve efficiency maturation. Furthers efforts have be made maturation and standardize semen-capacitating process.
Summary Ovarian stimulation with exogenous follicle stimulating hormone (FSH) has been used to increase the number of viable oocytes for laparoscopic oocyte recovery (LOR) in goats. The aim this study was evaluate effect two FSH protocols ovarian goats on expression pattern epidermal growth factor (EGF) receptor (EGFR) cumulus–oocyte complexes (COCs) recovered by LOR. After real-time qRT-PCR analysis, profiles morphologically graded COCs were compared prior and after vitro maturation (IVM) a...
Summary The present study investigated the effects of crotamine, a cell-penetrating peptide from rattlesnake venom, at different exposure times and concentrations, on both developmental competence gene expression ( ATP1A1, AQP3, GLUT1 GLUT3 ) in vitro fertilized (IVF) bovine embryos. In Experiment 1, presumptive zygotes were exposed to 0.1 μM crotamine for 6, 12 or 24 h control groups (vehicle IVF) included. 2, 0 (vehicle), 0.1, 1 10 h. Additionally, visualize uptake, embryos rhodamine...
Taking into account the importance of goats as transgenic models, well rarity copy number (CN) studies in farm animals, present work aimed to evaluate methodological strategies for accurate and precise transgene CN quantification using quantitative polymerase chain reaction (qPCR). Mouse goat lines human granulocyte‐colony stimulating factor were used. After selecting best genomic DNA extraction method be applied mouse samples, intra‐assay variations, accuracy precision quantifications...
The genetic diversity of Neotropical deer is increasingly jeopardized, owing to declining population size. Thus, the formation cryobanking somatic cells important for preservation these species using cloning. transformation into viable embryos has been hampered by a lack endangered oocytes. Accordingly, aim this study was produce brown brocket interspecific cell nuclear transfer (iSCNT), goat or cattle oocytes as cytoplasts, and elucidate embryo mitochondrial activity measuring expression...