A5047014091- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Animal Genetics and Reproduction
- Genetic and phenotypic traits in livestock
- Sperm and Testicular Function
- Effects of Environmental Stressors on Livestock
- Ovarian function and disorders
- Pluripotent Stem Cells Research
- Reproductive Health and Technologies
- Molecular Biology Techniques and Applications
- Birth, Development, and Health
- Genetic Mapping and Diversity in Plants and Animals
- CRISPR and Genetic Engineering
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive System and Pregnancy
- Renal and related cancers
- Rabbits: Nutrition, Reproduction, Health
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Biochemical Acid Research Studies
- Animal Diversity and Health Studies
- Allelopathy and phytotoxic interactions
- Milk Quality and Mastitis in Dairy Cows
- Leptospirosis research and findings
- Genetic and rare skin diseases.
Vitrogen (Brazil)
2014-2024
Biotech (Brazil)
2017-2024
Watanabe Applied Technology (Brazil)
2014-2024
Universidade de São Paulo
2000-2011
Universidade Estadual Paulista (Unesp)
1994-2000
Climate change is a reality and global surface temperature projected to rise substantially in the next 80 years. Agriculture practices will have adapt climate change, also help mitigate this effect using, among other strategies, forest conservation management. Silvopastoral systems been adopted tropical livestock areas but their benefits on thermal comfort reproductive performance of beef cows are not completely known. Therefore, our aims were compare microclimate silvopastoral intensive...
Oocyte quality is known to be a major cause of infertility in repeat-breeder (RB) and heat-stressed dairy cows. However, the mechanisms by which RB oocytes become less capable supporting embryo development remain largely unknown. Thus, aim this study was investigate whether decreased oocyte competence cows (RBs) during summer associated with an altered gene expression profile decrease mitochondrial DNA (mtDNA) copy number. Therefore, collected from heifers, non-RBs peak lactation (PLs), RBs...
Oocytes from dairy cattle and buffaloes have severely compromised developmental competence during summer. While analysis of gene expression is a powerful technique for understanding the factors affecting hindrance in oocytes, by real-time reverse transcription PCR (RT-PCR) relies on correct normalization reference genes showing stable expression. Furthermore, several studies found that commonly used as standards do not behave expected depending cell type experimental design. Hence, it...
The association of OPU-IVEP is an important instrument to drive genetic progress.In vitro embryo production (IVEP) has remarkably expanded in the last decade compared vivo production.Because high repeatability oocyte retrieval within oocyte-donors, studies exploring relationship between number oocytes recovered per OPU section with IVEP efficiency, as well field fertility (pregnancy results following transfer; P/ET) are extremely guide cow-donor selection and optimize reproduction efficiency...
Abstract Due to the exclusively maternal inheritance of mitochondria, mitochondrial genotypes can be coupled a particular nuclear genotype by continuous mating founder females and their female offspring males desired genotype. However, backcrossing is gradual procedure that, apart from being lengthy, cannot ascertain that genetic epigenetic changes will modify original Animal cloning transfer using host ooplasm carrying polymorphic genomes allows, among other biotechnology applications,...
Abstract We tested the hypothesis that biosensor capability of endometrium is mediated in part, by effect different cargo contained extracellular vesicles secreted conceptus during peri‐implantation period pregnancy. transferred Bos taurus embryos origin, vivo (high developmental potential (IV)), vitro (intermediate (IVF)), or cloned (low (NT)), into indicus recipients. Extracellular (EVs) recovered from Day 16 conceptus‐conditioned medium were characterized and their microRNA (miRNA)...
Cows fed high-protein diets may have impaired reproductive performance. Although the pathogenesis has not been completely elucidated, it appears that only uterus, but also follicle and oocyte, are affected by excessive plasma urea nitrogen (PUN) concentrations. Thus, objective was to determine effects of short-term feeding on competence bovine oocytes. Forty crossbred heifers (Bos indicus vs Bos taurus) were allocated two groups, namely CONTROL (maintenance diet) UREA diet supplemented with...
Carvacrol (C10H14O), an efficient phenolic antioxidant substance for several cell types, may become a useful female germ cells and embryo culture. This study investigates the effects of carvacrol supplementation on bovine oocytes in vitro maturation (IVM) production. In total, 1222 cumulus-oocyte complexes were cultured TCM-199+ alone (control treatment) or supplemented with at concentrations 3 µM (Carv-3), 12.5 (Carv-12.5), 25 (Carv-25). After IVM, subjected to fertilization production,...
Summary The embryonic developmental block occurs at the 8-cell stage in cattle and is characterized by a lengthening of cell cycle an increased number embryos that stop development. maternal-embryonic transition arises same resulting transcription many genes. Gene expression studies during this may contribute to understanding physiological mechanisms involved transition. Herein we identified genes differentially expressed between with high or low competence reach blastocyst using...
The aim of this study was to evaluate the effect age Nellore (Bos indicus) donors on efficiency in vitro embryo production (IVEP) and pregnancy rate. Thirty-six donors, including 11 female calves (13 ± 0.61 months), 17 prepubertal heifers (25 0.78 months) 8 cows (83 28 were submitted 3 procedures ovum pickup (OPU) random days estrous cycle at intervals 21 days. Caspase-3 IGFBP2 quantified oocytes blastocysts for evaluation oocyte quality. produced embryos vitrified (n = 445) transferred...
In early development, female embryos (XX) produce twice the transcripts of X-linked genes compared with male (XY). During course inactivation X chromosome equilibrates gene dosage, making development viable. Moreover, biotechnologies used for producing in vitro seem to work better embryos, it easier them reach blastocyst stage and allow complete gestation. We investigated expression three that are involved XIST, G6PD, HPRT, transcript interferon-tau, bovine blastocysts produced by nuclear...