A5011125178- Reproductive System and Pregnancy
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Reproductive Physiology in Livestock
- Pharmacological Effects and Assays
- RNA Research and Splicing
- RNA modifications and cancer
- Animal Nutrition and Physiology
- Electrochemical sensors and biosensors
University of Leeds
2020-2024
Teagasc - The Irish Agriculture and Food Development Authority
2018-2020
University College Dublin
2018-2020
Abstract We tested the hypothesis that biosensor capability of endometrium is mediated in part, by effect different cargo contained extracellular vesicles secreted conceptus during peri‐implantation period pregnancy. transferred Bos taurus embryos origin, vivo (high developmental potential (IV)), vitro (intermediate (IVF)), or cloned (low (NT)), into indicus recipients. Extracellular (EVs) recovered from Day 16 conceptus‐conditioned medium were characterized and their microRNA (miRNA)...
Interferon Tau (IFNT), the conceptus-derived pregnancy recognition signal in cattle, significantly modifies transcriptome of endometrium. However, endometrium also responds to IFNT-independent products. The aim this study was determine what proteins are produced by bovine conceptus that may facilitate process cattle. We analysed mass spectrometry present conceptus-conditioned media (CCM) after 6 h culture Day 16 conceptuses (n = 8) SILAC (arginine- and lysine-depleted supplemented with heavy...
Current bovine pregnancy detection methods are not reliable until at least day 28 post artificial insemination (AI). The estrous cycle is approximately 21 days; consequently, producers miss an opportunity to rebreed the next event. Therefore, commercial interest exists for discovery of novel biomarkers which could reliably detect status or before pregnancy. objective present study was use liquid chromatography tandem mass spectrometry (LC-MS/MS) perform a global, label-free, proteomics on...
ABSTRACT We tested the hypothesis that biosensor capability of endometrium is mediated in part, by effect different cargo contained extracellular vesicles secreted conceptus during peri-implantation period pregnancy. transferred Bos taurus embryos origin: In vivo (high developmental potential (IV)), vitro (intermediate (IVF)), or cloned (low (NT)), into indicus recipients. Extracellular (EVs) recovered from Day 16 conditioned medium were characterized and their microRNA (miRNA) sequenced...
A robust electrochemical assay for sorbitol dehydrogenase (SORDH) activity in milk was developed using voltammetry and chronocoulometry at bare polymer modified transducers. The motivation the work to evaluate potential of SORDH as an early biomarker bovine pregnancy sample matrix. is enzyme involved carbohydrate metabolism converting sorbitol, sugar alcohol form glucose, into fructose, with NAD+ a cofactor being simultaneously reduced NADH. optimised via direct NADH oxidation on glassy...
ABSTRACT In comparison to protein-coding genes, long non-coding RNAs (lncRNAs) are poorly conserved between species at the sequence level yet they often perform and essential regulatory roles. The lncRNA XIST mediates X chromosome inactivation (XCI) through interaction of numerous proteins, most which bind its repeat regions. Whilst is present across placental mammals, divergent. addition, timing mechanistic details process XCI also vary mammals. Here, we sought determine whether interactor...