A5007793353- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Force Microscopy Techniques and Applications
- Pluripotent Stem Cells Research
- Microfluidic and Bio-sensing Technologies
- Cell Adhesion Molecules Research
- Developmental Biology and Gene Regulation
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Microtubule and mitosis dynamics
- Toxic Organic Pollutants Impact
- Neurogenesis and neuroplasticity mechanisms
- Polymer Surface Interaction Studies
- Effects and risks of endocrine disrupting chemicals
- Micro and Nano Robotics
- nanoparticles nucleation surface interactions
- Microstructure and mechanical properties
University of Massachusetts Amherst
2017-2023
University of Massachusetts Boston
2021
Amherst College
2018
A DNA-based fluorescent probe to quantify the magnitude and distribution of tensile forces at cell–cell junctions.
Abstract Mechanical interactions between cells have been shown to play critical roles in regulating cell signaling and communications. However, the precise measurement of intercellular forces is still quite challenging, especially considering complex environment at cell–cell junctions. In this study, we report a fluorescence lifetime‐based approach image quantify molecular tensions. Using method, tensile among multiple ligand–receptor pairs can be measured simultaneously. We first validated...
Exposure to phthalate acid esters (PAEs) has been found have adverse effects on reproduction of human and animal. However, the semen quality are controversial. In this study, we collected paired blood sperm samples from patients (n = 103) at a medical clinic in Guangzhou, China, determine concentrations eight metabolites (m-PAEs). The concentration ranges ∑8m-PAEs were 2.7–55 0.99–17 ng/mL samples, respectively. MEHP (median, 3.6 0.55 semen) MnOP 3.4 0.69 predominant analytes both samples....
Fusion of cancer cells is thought to contribute tumor development and drug resistance. The low frequency cell fusion events the instability fused have hindered our ability understand molecular mechanisms that govern fusion. We demonstrated several breast lines can fuse into multinucleated giant in vitro, initiation longevity be regulated solely by biophysical factors. Dynamically tuning adhesive area patterned substrates, reducing cytoskeletal tensions pharmacologically, altering matrix...
Cells continuously experience and respond to different physical forces that are used regulate their physiology functions. Our ability measure these mechanical cues is essential for understanding the bases of various mechanosensing mechanotransduction processes. While multiple strategies have been developed study within two-dimensional (2D) cell culture monolayers, force measurement at cell-cell junctions in real three-dimensional (3D) models still pretty rare. Considering biological systems,...
A monolayer of highly motile cells can establish long-range orientational order, which be explained by hydrodynamic theory active gels and fluids. However, it is less clear how cell shape changes rearrangement are governed when the in mechanical equilibrium states motility diminishes. In this work, we report that rat embryonic fibroblasts (REF), confined circular mesoscale patterns on rigid substrates, transition from spindle shapes to more compact morphologies. Cells align radially only at...
Abstract Mechanical interactions between cells have been shown to play critical roles in regulating cell signaling and communications. However, the precise measurement of intercellular forces is still quite challenging, especially considering complex environment at cell–cell junctions. In this study, we report a fluorescence lifetime‐based approach image quantify molecular tensions. Using method, tensile among multiple ligand–receptor pairs can be measured simultaneously. We first validated...
Abstract Strain gradients widely exist in development and physiological activities. The directional movement of cells is essential for proper cell localization, migration responses to chemicals, rigidity, density, topography extracellular matrices have been well‐established. However; it unclear whether strain imposed on are sufficient drive migration. In this work, a programmable uniaxial stretch device developed that creates controllable without changing substrate stiffness or ligand...
ABSTRACT Strain gradients, a graded change in the percentage of deformation across continuous field by applying forces, widely exist development and physiological activities. The directional movement cells is essential for proper cell localization, migration responses to gradients chemicals, rigidity, density topography extracellular matrices have been well-established. However, it unclear whether strain imposed on are sufficient drive migration. In this work, we develop programmable...
SUMMARY Cells are physically contacting with each other. Direct and precise quantification of forces at cell–cell junctions is still challenging. Herein, we have developed a DNA-based ratiometric fluorescent probe, termed DNAMeter, to quantify intercellular tensile forces. These lipid-modified DNAMeters can spontaneously anchor onto live cell membranes. The DNAMeter consists two self-assembled DNA hairpins different force tolerance. Once the tension exceeds tolerance unfold hairpin, specific...
SUMMARY To develop into the central nervous system, neuroepithelial cells must first form a neural tube consisting of series patterned progenitor along anterior-posterior (AP) axis. Based on studies using model organisms, it has been revealed that AP spatial regionalization is dominated by gradients morphogens regulate retinoic acid (RA), sonic hedgehog (SHH), bone morphogenetic proteins (BMPs), and Wingless/int1 (WNT) signaling pathways. Recently, human pluripotent stem (hPSCs) were...
ABSTRACT It has been found that many types of cells form nematic symmetry on confined planar substrates. Such observation satisfactorily explained by modeling as crowded self-propelled rods. In this work, we report rat embryonic fibroblast (REF) when in circular mesoscale patterns, a new type where align radially at the boundary. Unlike NIH-3T3 and MDCK monolayers, REF monolayer presents supracellular actin gradient with isotropic meshwork. addition, contractile present strong adhesive...