Primary cilia (PC) are solitary, sensory organelles that critical for several signaling pathways. PC were detected by immunofluorescence of cultured cells and breast tissues. After growth 7 days in vitro, ∼70% fibroblasts 7–19% epithelial derived from benign (184A1 MCF10A). In 11 cancer cell lines, present at a low frequency four (from 0.3% to 4% cells), but absent the remainder. The lines with all basal B subtype, which is analogous clinical triple-negative subtype. Furthermore, decreased...

Gli1 is an established oncogene and its expression in Estrogen Receptor (ER) α negative triple breast cancers predictive of a poor prognosis; however, the biological functions regulated by cancer have not been extensively evaluated. Herein, was over-expressed or down-regulated (by RNA interference repressor form Gli3) ERα negative, human cell lines MDA-MB-231 SUM1315. Reduced these two resulted decrease migration invasion. over-expression increased invasion cells with corresponding increase...

In view of the poor prognosis laryngeal squamous cell carcinoma (LSCC) and functionality long non‑coding (lnc)RNA UCA1 in different types cancer, present study aimed to investigate role development progression LSCC. A total 90 patients with LSCC healthy subjects were enrolled study. Expression levels tumor tissues adjacent tissues, as well serum normal controls detected by reverse transcription‑quantitative polymerase chain reaction. Receiver operating characteristic curve analysis was...

Glycosylation of proteins plays multiple roles in cell-cell and cell-matrix interactions. Fucose is a monosaccharide associated with glycosylation events known to be over-expressed many malignant tumors. By using alpha-L-fucosidase (alpha-L-fase), glycosidase that specifically removes alpha-L-fucose (alpha-L-f), we have examined the potential effects defucosylation on tumor functions, focusing progression context interaction cells extracellular microenvironment. In this submission, report...

BACKGROUND:The clinical significance and biological function of long noncoding RNA SNHG12 have not been identified in laryngeal squamous cell carcinoma (LSCC). MATERIAL AND METHODS:Expression levels SNHG12, miR-129-5p, WWP1 LSCC tissues or cells were tested by RT-qPCR. MTT assay, flow cytometry, Transwell assay used to identify the progression vitro. Luciferase reporter was assess associations among WWP1, miR-129-5p. RESULTS:SNHG12 significantly overexpressed compared with adjacent normal...

MicroRNAs (miRs) serve important roles in the development of various types human cancer, including nasopharyngeal carcinoma (NPC). In present study, expression levels miR-663b NPC were investigated and its role underlying mechanisms examined. Reverse transcription-quantitative polymerase chain reaction was performed to assess tissues C666-1 cells. TargetScan applied predict putative targets dual luciferase reporter assay used confirm predictions. To investigate NPC, cell line transfected...

Objective. To investigate the biological role and potential mechanism of miR-25 in nasopharyngeal carcinoma. Methods. The expression carcinoma cell lines was detected by qRT-PCR. effect inhibition on proliferative activity line HONE-1 examined CCK-8 method. Flow cytometry used to detect apoptosis rate HONE-1. miRNA target gene prediction site TargetScan predicts protein action miR-124 verifies whether interacts with luciferase assay, qPCR, Western experiments. inhibitor egg plasmids were...

Primary cilia are non-motile, microtubule-based appendages extending from the surfaces of most vertebrate cells.The function primary is cell-type dependent.However, they known to serve as sensors extracellular environment by sensing mechanical forces and chemical constituents in several different cell types.They also critical platforms for signaling pathways, including hedgehog platelet derived growth factor (PDGF) signaling, maintaining polarity.While present on many types normal cells,...

Primary cilia are microtubule-based organelles that regulate smoothened-dependent activation of the GLI transcription factors in canonical hedgehog signaling. In many cancers, primary markedly decreased or absent. The lack may inhibit alter signaling and, thereby, interfere cellular responsiveness to modulators smoothened activity. Clinical trials antagonists for cancer treatment have shown best response basal cell carcinomas, with limited other solid tumors. To determine whether presence...

Abstract Objective Ventral anterior homeobox 2 (VAX2) gene is a key regulating factor for the development of ventral region eye, and has recently attracted much attention from cancer treatment field. Our study aimed to explore effect VAX2 on papillary thyroid carcinoma (PTC). Methods We determined expression levels in PTC based The Cancer Genome Atlas (TCGA) database. then assessed prognosis patients with PTC, analyzed association between clinicopathological characteristics. Subsequently, we...

Objective: To investigate the expression and clinical significance of long non-coding RNA FOXD2-AS1 in laryngeal squamous cell carcinoma its effect on cancer proliferation. Method: Real-time quantitative polymerase chain reaction(RT-qPCR) was used to detect 85 cases carcinoma. One-way ANOVA determine relationships between clinicopathological parameters The prognostic head neck explored using bioinformatics technology. SiRNA interfere TU686 cells. MTT clonal formation assay were biological...

The present study aimed to establish an animal model of the osteofascial compartment syndrome (OFCS) in hind legs rabbits, and repair early nerve injury using self-polymerized peptide nanofiber material that was laced with growth factors (NGFs). An established tourniquet method. intrafascial pressure rabbit at different time points measured after successful modeling. Western blotting used assess proliferation cells injury. After incision decompression, group implanted combination NGFs sites...

Abstract Background: Three dimensional (3D) culture is a more physiologically relevant method to model cell behavior in vitro than two culture. 3D modeling of cancer particular importance drug development where predicting vivo effectiveness challenging. Not only the structure important for proper but response from surrounding microenvironment, including extracellular matrix (ECM) and fibroblasts, also necessary accurately predict response. A major hindrance loss viability due nutrient...

Abstract Background: Gli1 is a transcription factor and oncogene that was first described in the context of Hedgehog signaling. We have previously shown nuclear localization predictive poor prognosis estrogen receptor (ER)-negative (ER-), but not ER-positive (ER+) breast carcinomas (BrCa) [Xu et al. Breast Cancer ResTreat 2010:59]. In addition, we demonstrated overexpression ER- BrCa cell lines increases migration invasion and, conversely, silencing decreases migration, metastasis [Kwon...

Background: This study was designed to investigate the expression of centromere protein F (CENPF) in laryngeal squamous cell carcinoma (LSCC), and assess whether CENPF could be a potential biomarker for LSCC. Methods: GEO datasets were analyzed tissue microarray (TMA) using composite score (CES). Finally, we silenced Hep-2 FaDu cells explore its mechanism action Results: The TMA revealed is upregulated LSCC compared normal tissues, also N2-N3 N0-N1 associated with differentiation, prognosis...