A5017286321- Genetics, Aging, and Longevity in Model Organisms
- Microtubule and mitosis dynamics
- Endoplasmic Reticulum Stress and Disease
- Cellular transport and secretion
- Erythrocyte Function and Pathophysiology
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- Autophagy in Disease and Therapy
- Amyotrophic Lateral Sclerosis Research
- Phosphodiesterase function and regulation
- Parkinson's Disease Mechanisms and Treatments
- Neuroscience and Neuropharmacology Research
- Anxiety, Depression, Psychometrics, Treatment, Cognitive Processes
- Photoreceptor and optogenetics research
- Neurological disorders and treatments
- Axon Guidance and Neuronal Signaling
- Glaucoma and retinal disorders
- RNA modifications and cancer
- Mind wandering and attention
- Genetic Neurodegenerative Diseases
- Cytomegalovirus and herpesvirus research
- Mental Health Research Topics
- Pancreatic function and diabetes
- Mitochondrial Function and Pathology
- Protein Kinase Regulation and GTPase Signaling
Seoul National University
2024
Yale University
2020-2023
Johns Hopkins Medicine
2021
Johns Hopkins University
2021
ABSTRACT Cell invasion through basement membrane (BM) barriers is important in development, immune function and cancer progression. As BM often stochastic, capturing gene expression profiles of actively invading cells vivo remains elusive. Using the stereotyped timing Caenorhabditis elegans anchor cell (AC) invasion, we generated an AC transcriptome during breaching. Through a focused RNAi screen transcriptionally enriched genes, identified new regulators, including translationally...
Abstract We previously identified a causal link between rare patient mutation in DISC1 (disrupted-in-schizophrenia 1) and synaptic deficits cortical neurons differentiated from isogenic patient-derived induced pluripotent stem cells (iPSCs). Here we find that transcripts related to phosphodiesterase 4 (PDE4) signaling are significantly elevated human iPSCs with the inhibition of PDE4 or activation cAMP pathway functionally rescues deficits. further generated knock-in mouse line harboring...
Protein aggregation is a hallmark of many neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Although mutations in TARDBP, encoding TDP-43, account for less than 1% all ALS cases, TDP-43-positive aggregates are present nearly patients, patients with sporadic (sALS) or carrying other familial (fALS)-causing mutations. Interestingly, TDP-43 inclusions also subsets frontotemporal dementia, Alzheimer's disease, and Parkinson's disease; therefore, methods activating...
Genetic parasites, including viruses and transposons, exploit components from the host for their own replication. However, little is known about virus-transposon interactions within cells. Here, we discover a strategy where human cytomegalovirus (HCMV) hijacks L1 retrotransposon encoded protein during its replication cycle. HCMV infection upregulates expression by enhancing both of L1-activating transcription factors, YY1 RUNX3, chromatin accessibility promoter regions. Increased expression,...
Axonal transport is key to neuronal function. Efficient requires specific motor-cargo association in the soma, yet mechanisms regulating this early step remain poorly understood. We found that EBP-1,
https://doi.org/10.1038/s41467-021-23263-0
Abstract Protein aggregation is a hallmark of many neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Although mutations in TARDBP , encoding TDP-43, account for less than 1% all ALS cases, TDP-43-positive aggregates are present nearly patients, patients with sporadic (sALS) or carrying other familial (fALS)-causing mutations. Interestingly, TDP-43 inclusions also subsets frontotemporal dementia, Alzheimer’s disease, and Parkinson’s disease; therefore, methods...
Precise synaptic connectivity defines neuronal circuits. Synapse formation is locally determined by transmembrane proteins, yet material synthesized remotely and undergoes processive transport in axons. How local synaptogenic signals intercept cargo to promote its delivery synapse unknown. We found that control of at microtubule minus-ends mediates pro- anti-synaptogenic activities presynaptic Neurexin Frizzled C. elegans, identified the atypical kinesin VAB-8/KIF26 as a key molecule this...
Abstract A periodic lattice of actin rings and spectrin tetramers scaffolds the axonal membrane. How is delivered to this structure scale its size that growing axon unknown. We found endogenous spectrin, visualized with singe resolution in vivo , hotspots support expansion at rates set by stretch-growth. Unlike other cytoskeletal proteins, whose apparent slow movement consists intermittent bouts fast movements, moves slowly processively. identified a pair coiled coil proteins mediate linking...
ABSTRACT Cell invasion through basement membrane (BM) barriers is important in development, immune function, and cancer progression. As BM often stochastic, capturing gene expression profiles of cells actively transmigrating vivo remains elusive. Using the stereotyped timing C. elegans anchor cell (AC) invasion, we generated an AC transcriptome during breaching. Through a focused RNAi screen transcriptionally enriched genes, identified new regulators, including TCTP (Translationally...
Abstract Precise synaptic connectivity defines neuronal circuits. Synapse formation is locally determined by transmembrane proteins, yet material synthesized remotely and undergoes processive transport in axons. How local synaptogenic signals intercept cargo to promote its delivery synapse unknown. We found that control of at microtubule (MT) minus-ends mediates pro- anti-synaptogenic activities presynaptic Neurexin Frizzled C. elegans , identified the atypical kinesin VAB-8/KIF26 as a key...