A5045918855- Natural product bioactivities and synthesis
- NF-κB Signaling Pathways
- Osteoarthritis Treatment and Mechanisms
- Peroxisome Proliferator-Activated Receptors
- Cholesterol and Lipid Metabolism
- Cell death mechanisms and regulation
- Inflammatory mediators and NSAID effects
- Curcumin's Biomedical Applications
- Lipid metabolism and biosynthesis
- RNA Interference and Gene Delivery
- Phytoestrogen effects and research
- Erythrocyte Function and Pathophysiology
- Tannin, Tannase and Anticancer Activities
- Autophagy in Disease and Therapy
- Synthetic Organic Chemistry Methods
- Phytochemistry and Biological Activities
- Bioactive natural compounds
- Oral microbiology and periodontitis research
- Biochemical Acid Research Studies
- Biochemical and Molecular Research
- Bioactive Natural Diterpenoids Research
- Sphingolipid Metabolism and Signaling
- Free Radicals and Antioxidants
- Metabolism, Diabetes, and Cancer
- Bone Metabolism and Diseases
Chosun University
2015-2024
Jeong-Yeon Seo, Tae-Hyeon Kim, Kyeong-Rok Kang, HyangI Lim, Moon-Chang Choi, Do Kyung Hong Sung Chun, Heung-Joong Sun-Kyoung Yu, and Jae-Sung Kim. Mol. Cells -0001;0:. https://doi.org/10.14348/molcells.2023.2149
25-hydroxycholesterol (25-HC) is an oxysterol synthesized from cholesterol by cholesterol-25-hydroxylase during metabolism. The aim of this study was to verify whether 25-HC induces oxiapoptophagy in fibroblasts. not only decreased the survival L929 cells, but also increased number cells with condensed chromatin and altered morphology. Fluorescence-activated cell sorting results showed that there a dose-dependent increase apoptotic populations upon treatment 25-HC. 25-HC-induced death...
25-hydroxycholesterol (25-HC) plays important roles in lipid metabolism, inflammatory responses, and apoptosis, but its pathophysiological association with osteoporosis (OP) has not been verified osteoblasts. Hence, we studied the linkage underlying cellular mechanisms of 25-HC human osteoblast-like MG-63 cells an ovariectomy-induced osteoporotic mouse model.To investigate between 25-HC-induced osteoblast oxiapoptophagy OP, ELISA assay, MTT cell live/dead staining, hematoxylin eosin DAPI...
Osteoarthritis (OA) is the most common degenerative joint disease with chronic pain caused by progressive degeneration of articular cartilage at synovial joints. Acteoside, a caffeoylphenylethanoid glycoside, has various biological activities such as antimicrobial, anti‐inflammatory, anticancer, antioxidative, cytoprotective, and neuroprotective effect. Further, oral administration acteoside high dosage does not cause genotoxicity. Therefore, aim present study to verify anticatabolic effects...
7α,25-dihydroxycholesterol (7α,25-DHC) is an oxysterol synthesized from 25-hydroxycholesterol by cytochrome P450 family 7 subfamily B member 1 (CYP7B1) and a monooxygenase (oxysterol-7α-hydroxylase) expressed under inflammatory conditions in various cell types. In this study, we verified that 7α,25-DHC-induced oxiapoptophagy mediated apoptosis, oxidative stress, autophagy L929 mouse fibroblasts. MTT assays live/dead staining revealed cytotoxicity was increased 7α,25-DHC cells....
Metformin is an anti-diabetic drug that exerts protective effects against neurodegenerative diseases. In this study, we investigated the of metformin manganese (Mn)-induced cytotoxicity associated with Parkinson's disease-like symptoms in N27-A dopaminergic (DA) cells. (0.1–1 mM) suppressed Mn (0.4 mM)-induced cell death a concentration-dependent manner. pretreatment effectively Mn-mediated increase levels oxidative stress markers, such as reactive oxygen species (ROS) and thiobarbituric...
Demethoxycurcumin (DMC) is a curcumin analog with antitumor properties. However, its effects have not been investigated in human head and neck squamous cell carcinoma (HNSCC). The aim of the present study was to verify effect cellular signaling pathways DMC FaDu HNSCC cells.3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Live/Dead staining, hematoxylin eosin DAPI FACS, western blotting, caspase-3 activity assay, nuclear translocation were performed apoptosis pathway...
The physiological effects and cellular mechanism of 25-hydroxycholesterol (25-HC), which is an oxysterol synthesized from cholesterol by cholesterol-25-hydroxylase (CH25H) expressed under inflammatory conditions, are still largely unknown during odontoclastogenesis. This study aimed to evaluate 25-HC-induced odontoclastogenesis its mechanisms in odontoblast-like MDPC-23 cells.To investigate cells mechanism, haemotoxylin eosin staining, tartrate-resistant acid phosphatase (TRAP) dentine...
Acacetin, which is present in damiana (Turnera diffusa ) and black locust (Robinia pseudoacacia ), has several pharmacologic activities such as antioxidant, anti-inflammatory, anti-proliferative effects on cancer cells. However, the effect of acacetin head neck cancers not been clearly established. This study aimed to examine cell growth apoptosis induction FaDu human pharyngeal carcinoma These were investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, Live/Dead...
The aim of the present study was to investigate physiological role nicotinamide phosphoribosyltransferase (NAMPT) associated with odontogenic differentiation during tooth development in mice. Mouse dental papilla cell-23 (MDPC- 23) cells cultured media were stimulated specific NAMPT inhibitor, FK866, and Visfatin for up 10 days. evaluated after 0, 4, 7, Cell viability measured using 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide assay. mineralization assay performed by...
Abstract Objectives To verify the anti-catabolic effects of acteoside [α-L-rhamnosyl-(1->3)-β-D-glucoside] against osteoarthritis and its signaling pathway. Methods Primary rat chondrocytes were isolated enzymatically from articular cartilage knee joint. Cytotoxicity was assessed by MTT Cell Live/Dead assay. Proteoglycan content measured dimethylmethylene blue The proteoglycan loss histological analysis using safranin-O & fast green staining after ex vivo organ culture cartilage....
Demethoxycurcumin (DMC), which is a curcuminoid found in turmeric, has anti-proliferative effects on cancer cells. However, the effect of DMC osteosarcoma not been established. The aim this study was to examine cell growth and apoptosis induction MG-63 human This investigated using 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromid assay, Live/Dead 4’, 6-diamidino-2-phenylindole staining, immunoblotting induced death dosedependent manner, with an estimated IC50 value 54.4 μM....
Hyperoside (quercetin 3-o-β-d-galactopyranoside) is a flavonoid glycoside with antidepressant, anti-inflammatory, and anti-cancer effects.However, it not known whether effective for oral cancer.This study aimed to investigate the effects of hyperoside on induction apoptosis in FaDu human pharyngeal carcinoma cells.It employed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, live/dead staining, 4′,6-diamidino-2-phenylindole (DAPI) hematoxylin eosin western blotting...
Kyeong-Rok Kang, Jae-Sung Kim, HyangI Lim, Jeong-Yeon Seo, Jong-Hyun Park, Hong Sung Chun, Sun-Kyoung Yu, Heung-Joong Chun and Do Kyung Kim. Korean J Physiol Pharmacol 2022;26:447-56. https://doi.org/10.4196/kjpp.2022.26.6.447
Alpha-lipoic acid (ALA) is a naturally occurring antioxidant and has been previously used to treat diabetes cardiovascular disease. However, the autophagy effects of ALA against oxidative stress-induced dopaminergic neuronal cell injury remain unclear. The aim this study was investigate role in apoptosis stress SH-SY5Y human line. We examined phenotypes using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay (cell viability/proliferation), 4′,6-diamidino-2-phenylindole...