A5015216387- Cell death mechanisms and regulation
- Bioactive Compounds and Antitumor Agents
- TGF-β signaling in diseases
- Nanoparticles: synthesis and applications
- Genetics, Aging, and Longevity in Model Organisms
- Graphene and Nanomaterials Applications
- Carbon Nanotubes in Composites
- Spaceflight effects on biology
- Nicotinic Acetylcholine Receptors Study
- RNA Interference and Gene Delivery
- Protease and Inhibitor Mechanisms
- Sperm and Testicular Function
- HER2/EGFR in Cancer Research
- Natural product bioactivities and synthesis
- Cancer, Hypoxia, and Metabolism
- Aortic aneurysm repair treatments
- Cancer, Lipids, and Metabolism
- Cancer Cells and Metastasis
- Mitochondrial Function and Pathology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Folate and B Vitamins Research
- Flavonoids in Medical Research
- Cancer-related gene regulation
- Phytochemical compounds biological activities
- Infectious Aortic and Vascular Conditions
Sapienza University of Rome
2007-2017
Policlinico Umberto I
2009-2015
Cambridge University Press
2010
New York University Press
2010
In recent years, studies of cancer development and recurrence have been influenced by the stem cells (CSCs)/cancer-initiating (CICs) hypothesis. According to this, is sustained highly positioned, chemoresistant with extensive capacity self renewal, which are responsible for disease relapse after chemotherapy. Growth as three-dimensional non-adherent spheroids regarded a useful methodology enrich endowed CSC-like features. We recently reported that cell cultures derived from malignant pleural...
Abstract: Previous investigations demonstrated that melatonin exerts an oncostatic action on estrogen‐responsive breast cancer, both in vitro and vivo. Nevertheless, the pro‐apoptotic effect of is still a matter debate. An experimental study was undertaken to focus melatonin‐related apoptosis identify apoptotic pathways involved. Whole cell‐count, flow‐cytometry analysis proteins involved [p53, p73, murine double minute 2 (MDM2), caspases‐9,‐7,‐6, cleaved‐poly ADP ribose polymerase (PARP),...
Microgravity exerts dramatic effects on cell morphology and functions, by disrupting cytoskeleton adhesion structures, as well interfering with biochemical pathways gene expression. Impairment of cells behavior has both practical theoretical significance, given that investigations mechanisms involved in microgravity-mediated may shed light how biophysical constraints cooperate shaping complex living systems. By exposing breast cancer MDA-MB-231 to simulated microgravity (~0.001 g), we...
Microgravity influences cell differentiation by modifying the morphogenetic field in which stem cells are embedded. Preliminary data showed indeed that committed to selective when exposed real or simulated microgravity. Our study provides evidence a similar event occurs cancer (CSCs) cultured In same time, significant increase apoptosis was recorded: those point out microgravity rescues CSCs from their relative quiescent state, inducing lose stemness features, as documented decrease ALDH and...
Consumption of grape seed extract (GSE) is widely marketed as a dietary supplement and considered safe for human health. Nevertheless, the analytical composition GSE from different cultivars, growing in special agronomic constraints, differs greatly flavan-3-ols content. The major concern with studies lack availability uniformly standardised preparations, which raises an important question whether samples have comparable activity trigger same mechanisms action on given biological system....
// Alessia Noto 1,2 , Claudia De Vitis 2,3 Giuseppe Roscilli 1,4 Luigi Fattore Debora Malpicci 2 Emanuele Marra 4 Laura Luberto 3 Antonio D'Andrilli 6 Pierpaolo Coluccia Maria Rosaria Giovagnoli 1,6 Nicola Normanno 7 Ruco Aurisicchio 4,5 Rita Mancini Gennaro Ciliberto 1 Dipartimento di Medicina Clinica e Molecolare, Sapienza Universita' Roma, Italy; Chirurgia "P. Valdoni", Università Roma; Sperimentale Clinica, degli Studi Catanzaro "Magna Graecia", Catanzaro, Italy Takis s.r.l., Via Castel...
Grape seed extract (GSE) from Italia, Palieri and Red Globe cultivars inhibits cell growth induces apoptosis in Caco-2 human colon cancer cells a dose-dependent manner. In order to investigate the mechanism(s) supporting apoptotic process, we analysed reactive oxygen species (ROS) production, intracellular Ca 2+ handling extracellular signal-regulated kinase (ERK) activation. Upon exposure GSE, ROS levels increased cells, concomitantly with ERK inactivation. As activity is thought be...
Cigarette smoking is a recognized risk factor for colon cancer and nicotine, the principal active component of tobacco, plays pivotal role in increasing cell growth survival. The aim this study was to determine effect nicotine on cellular Caco‐2 HCT‐8 migration invasion, focusing epithelial mesenchymal transition (EMT) induction, COX‐2 pathway involvement. In both these lines, treatment with increased expression release its enzymatic product PGE 2 . Moreover, nicotine‐stimulated cells showed...
// Luigi Fattore 1, 2 , Debora Malpicci 2, 3 Emanuele Marra 4 Francesca Belleudi 5, 6 Alessia Noto Claudia De Vitis 5 Maria Elena Pisanu Pierpaolo Coluccia Rosa Camerlingo 1 Giuseppe Roscilli 4, Antoni Ribas 7 Arianna Di Napoli 8 Rosaria Torrisi 6, Aurisicchio Paolo Antonio Ascierto Rita Mancini Gennaro Ciliberto Istituto Nazionale per lo Studio e la Cura dei Tumori "Fondazione G. Pascale", Naples, Italy Dipartimento di Chirurgia "P. Valdoni", Sapienza Università Roma, Rome, Medicina...
Activation of Fas signaling has been associated with the development cardiomyocyte hypertrophy. In present study, we investigated effects increased expression c-Flip, a natural modulator receptor signaling, in mouse model cardiac growth response to pressure overload.A transgenic overexpressing c-Flip heart was generated FVB/N strain. Echocardiographic, hemodynamic, histological and molecular analyses were carried out under basal conditions after transverse aortic constriction (TAC)-induced...
This study shows that forcing c-Flip overexpression in undifferentiated skeletal myogenic cells vivo results early aging muscle phenotype. In the transgenic mice, adult histology, histochemistry and biochemistry show strong alterations: reduction of fibers size mass, mitochondrial abnormalities, increase protein oxidation apoptosis markers reduced AKT/GSK3β phosphorylation. infant, higher levels Pax-7, PCNA, P-ERK active-caspase-3 were observed, indicating enhanced proliferation concomitant...