A5062634645- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
- Thyroid Cancer Diagnosis and Treatment
- Viral Infectious Diseases and Gene Expression in Insects
- Immunotherapy and Immune Responses
- Hippo pathway signaling and YAP/TAZ
- Developmental Biology and Gene Regulation
- Mechanisms of cancer metastasis
- Inflammasome and immune disorders
- Chronic Lymphocytic Leukemia Research
- T-cell and B-cell Immunology
- Animal Behavior and Reproduction
- Peptidase Inhibition and Analysis
- S100 Proteins and Annexins
- Immunodeficiency and Autoimmune Disorders
- Neurobiology and Insect Physiology Research
- Cancer-related Molecular Pathways
- BRCA gene mutations in cancer
- Signaling Pathways in Disease
- Protein Kinase Regulation and GTPase Signaling
- Cancer, Hypoxia, and Metabolism
- Galectins and Cancer Biology
- Angiogenesis and VEGF in Cancer
- Extracellular vesicles in disease
The Ohio State University Wexner Medical Center
2014-2017
Diabetes Australia
2017
The Ohio State University
2006-2016
St. Jude Children's Research Hospital
2010
Interface (United States)
2010
Metastasis suppressors are key regulators of tumor growth, invasion, and metastases. Loss metastasis has been associated with aggressive behaviors metastatic progression. We previously showed that regulator calcineurin 1, isoform 4 (RCAN1-4) was upregulated by the KiSS1 suppression pathway could inhibit cell motility when overexpressed in cancer cells. To test effects endogenous RCAN1-4 loss on thyroid vivo, we developed knockdown stable Subcutaneous xenograft models demonstrated promotes...
Analysis of cells in culture has made substantial contributions to biological research. The versatility and scale vitro manipulation new applications such as high-throughput gene silencing screens ensure the continued importance cell-culture studies. In comparison mammalian systems, Drosophila cell is underdeveloped, primarily because there no general genetic method for deriving lines. Here we found expression conserved oncogene RasV12 (a constitutively activated form Ras) profoundly...
Immune dysregulation during sepsis is poorly understood, however, lymphocyte apoptosis has been shown to correlate with poor outcomes in septic patients. The inflammasome, a molecular complex which includes caspase-1, essential the innate immune response infection and also important induced apoptosis. Our group recently demonstrated that endotoxin-stimulated monocytes release microvesicles (MVs) containing caspase-1 are capable of inducing We sought determine if MVs being released into blood...
Abstract Purpose: Activation of Toll-like receptors (TLR) 7 and 8 by engineered agonists has been shown to aid in combating viruses tumors. Here, we wished test the effect TLR7/8 activation on monocyte Fcγ receptor (FcγR) function, as they are critical mediators antibody therapy. Experimental Design: The agonist R-848 cytokine production antibody-dependent cellular cytotoxicity human peripheral blood monocytes was tested. Affymetrix microarrays were done examine genomewide transcriptional...
SHIP and SHIP-2 are inositol phosphatases that regulate FcγR-mediated phagocytosis through catalytic as well non-catalytic mechanisms. In this study we have used two-dimensional fluorescence difference gel electrophoresis (DIGE) analysis to identify downstream signaling proteins uniquely associate with or upon FcγR clustering in human monocytes. We identified LyGDI a binding partner of SHIP, associating inducibly the SHIP/Grb2/Shc complex. Immunodepletion competition experiments recombinant...
In vivo analysis of Drosophila melanogaster has enhanced our understanding many biological processes, notably the mechanisms heredity and development. While in mutants been a strength field, analyzing fly cells culture is valuable for cell biological, biochemical whole genome approaches which large numbers homogeneous are required. An efficient genetic method to derive lines using expression an oncogenic form Ras (RasV12) developed. Mutations tumor suppressors, known cause hyperproliferation...
Abstract Differential gene expression is the major mechanism underlying development of specific body regions. Here we assessed role genes differentially expressed in Drosophila wing imaginal disc, which gives rise to two distinct adult structures: wall and wing. Reverse genetics was used test function uncharacterized first identified a microarray screen as having high levels presumptive Such could participate elaborating morphological characteristics The activity modulated using...
Abstract Context The primary and definitive treatment of medullary thyroid cancer (MTC) is surgical resection. Recurrent or residual disease typically a result incomplete removal. Objective Our objective to develop compound that assists in intraoperative visualization cancer, which would have the potential improve cure rates outcomes. Results We report biological characterization Compound-17, labeled with IRdye800, allowing fluorescent MTC mouse models. found agent has high affinity for two...
Abstract Fcγ receptor (FcγR) clustering on monocytes/macrophages leads to phagocytosis, cytokine release and other responses designed clear the antibody-opsonized targets. Toll-like Receptor 2 activation elicits strong proinflammatory responses, so we explored possibility that ligands for TLR2 could indirectly strengthen FcγR activity. We treated human peripheral blood monocytes (PBM) with agonist Pam2CSK4 found significantly enhanced FcγR-mediated production phagocytosis. Further...
Abstract Fcγ receptors (FcγR) are major effectors of antibody therapy so it is critical to seek new ways enhance their effectivness. Toll-like (TLR) recognize a wide range microbial components, eliciting cellular responses such as cytokine production and generation antimicrobial factors. Among these receptors, TLR2 recognizes both bacterial fungal components. Given the strong proinflammatory activation, we explored possibility that agonists could strengthen FcγR activity. Human peripheral...