A5005867158- Cancer-related Molecular Pathways
- Mobile Health and mHealth Applications
- PI3K/AKT/mTOR signaling in cancer
- Cardiovascular Health and Risk Factors
- Nutritional Studies and Diet
- Agriculture Sustainability and Environmental Impact
- Nitric Oxide and Endothelin Effects
- Physical Activity and Health
- Protein Degradation and Inhibitors
- Extracellular vesicles in disease
- Pharmacogenetics and Drug Metabolism
- Receptor Mechanisms and Signaling
- Computational Drug Discovery Methods
- Protein Kinase Regulation and GTPase Signaling
- Hippo pathway signaling and YAP/TAZ
- Colorectal Cancer Treatments and Studies
- Obesity, Physical Activity, Diet
- Head and Neck Cancer Studies
- MicroRNA in disease regulation
- Cancer Treatment and Pharmacology
- Oral Health Pathology and Treatment
- Redox biology and oxidative stress
- CRISPR and Genetic Engineering
- Plant biochemistry and biosynthesis
- Cardiovascular Health and Disease Prevention
The University of Sydney
2018-2024
RKDF University
2024
The Ohio State University
2001-2012
University of Kentucky
2008
Indian Institute of Chemical Biology
1995-2006
Center for Systems Biology
2004
Lung Institute
2004
Duke University
2001
National Institute of Allergy and Infectious Diseases
2001
Cold-induced vasoconstriction in cutaneous blood vessels is mediated part by increased activity of vascular smooth muscle α2-adrenoceptors (VSM α2-ARs). In mouse arteries, α2C-ARs are normally silent at 37°C but mediate cold-induced augmentation α2-AR responsiveness. transfected HEK293 cells, this functional rescue translocation from the Golgi to plasma membrane. Experiments were performed determine role Rho/Rho kinase signaling process. Inhibition Rho (fasudil, Y27632 or H-1152) did not...
Cold constricts cutaneous blood vessels by selectively increasing the activity of smooth muscle alpha2-adrenoceptors (alpha2-ARs). In mouse tail arteries, alpha2-AR constriction is mediated alpha2A-ARs at 37 degrees C, whereas cold-induced augmentation in entirely alpha2C-ARs. causes translocation alpha2C-ARs from trans-Golgi to plasma membrane, activation RhoA and Rho kinase. The present experiments analyzed mechanisms underlying these responses. Mouse arteries were studied a pressure...
Introduction: The recent multi-nation outbreaks of human monkeypox in non-endemic areas have created an emerging public health issue. Medical students who will become future healthcare providers are directly associated with community people and can easily sensitize the general population, so it is crucial to assess their degree knowledge attitudes regarding recently infections or pathogens. However, studies on medical students' perception virus scarce Saudi Arabia. Therefore, objective this...
Experiments were performed to determine the role of reactive oxygen species (ROS) in regulating vascular smooth muscle cell (VSMC) phenotype. After quiescence, cultured human VSMCs increased their expression differentiation proteins (alpha-actin, calponin, and SM1 SM2 myosin), but not beta-actin. ROS activity, determined using H(2)O(2)-sensitive probe dichlorodihydrofluorescein (DCF), remained high quiescent cells was inhibited by catalase (3000 U/mL) or N-acetylcysteine (NAC, 2 20 mmol/L)....
Raynaud's phenomenon, which is characterized by intense cold-induced constriction of cutaneous arteries, more common in women compared with men. Cold-induced mediated part enhanced activity alpha(2C)-adrenoceptors (alpha(2C)-ARs) located on vascular smooth muscle cells (VSMs). Experiments were therefore performed to determine whether 17beta-estradiol regulates alpha(2C)-AR expression and function VSMs. 17beta-Estradiol (0.01-10 nmol/l) increased the protein gene promoter human cultured...
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Arteriolar vascular smooth muscle cells (VSMCs) are mechanosensitive, constricting to elevations in transmural pressure (P TM ). The goal of the present study was determine using mouse isolated tail arterioles and arteries whether oxidant signaling regulates this myogenic response. In response P elevation, VSMCs but not generated constriction increased reactive oxygen species (ROS) activity (using H 2 O -sensitive probe dichlorodihydrofluorescein). Arterioles had expression NADPH oxidase...
Analysis of cells in culture has made substantial contributions to biological research. The versatility and scale vitro manipulation new applications such as high-throughput gene silencing screens ensure the continued importance cell-culture studies. In comparison mammalian systems, Drosophila cell is underdeveloped, primarily because there no general genetic method for deriving lines. Here we found expression conserved oncogene RasV12 (a constitutively activated form Ras) profoundly...
Previous research examining the effects of omnivorous and plant-based diets on cardiovascular diseases (CVD) outcomes has produced inconsistent findings, importance diet quality is overlooked. Our study aimed to develop a novel heart-protective index assess association high-quality with CVD incidence mortality. This included 192,274 participants in UK Biobank without at baseline who completed 24-hour recall Oxford WebQ questionnaire. Using questionnaire, we developed validated score (HPDS)...
Cold increases cutaneous vasoconstriction by unmasking the contractile activity of α 2C -adrenoceptors (α -ARs) in vascular smooth muscle cells (VSMCs), which is mediated cold-induced mobilization -ARs from transGolgi to cell surface. The expression human VSMCs under dual regulation cyclic AMP: gene transcription inhibited AMP acting through protein kinase A but increased exchange directly activated (EPAC) and GTP-binding Rap1. Experiments were performed further characterize Rap1 signaling...
Background: The predictive value of codon 72 arginine homozygosity at the p53 gene for human papilloma virus associated cervical cancer risk remains inconclusive. It has also been proposed that inheritance specific germline haplotypes based on three biallelic polymorphisms (intron 3 16 bp duplication, Bst UI (Arg/Pro), and intron 6 Nci I restriction fragment length polymorphism nucleotide 13494) is a better predictor various risks. Aims: To determine genotype haplotype frequency these in 61...
Aim: To investigate the complex interplay between human papilloma virus (HPV) infection and p53 gene alteration in 92 head neck squamous cell carcinoma (HNSCC) 28 leukoplakia samples from eastern India. Methods: DNA isolated patient was subjected to HPV detection, loss of heterozygosity (LOH) analysis chromosome 17p region harbouring , genotyping at codon 72 locus sequencing entire identify somatic mutations. Codon heterozygotes carrying mutation were further cloned resequenced allele...
Previously, we showed that Abl family tyrosine kinases are activated by growth factors, and is required for transition from G1 to S phase during PDGF-mediated proliferation. Here, show the SHP-2 phosphatase, which acts promote proliferation in response cytokines a novel substrate of endogenous factor-mediated cellular Using pharmacological inhibitor RNAi, phosphorylate on Y580, induce sustained activation ERK factor stimulation fibroblasts. Consistent with these data, Abl-dependent since...
This study assesses how effective gamification in smartphone apps is at enhancing lifestyle and cardiometabolic health adults risk of cardiovascular disease. Using a systematic review six databases, it looked trials that compared gamified traditional interventions. Although scored highly for functionality, averaging 4.07 rating, they lacked focus on user engagement. The reveals can aid achievable changes improve factors, providing insights future digital approaches targeting CVD reduction.
Abstract The role of 3 p53 polymorphisms (16 bp duplication at intron 3, codon 72 Arg/Pro and 6 Nci I RFLP np 13494) as potential markers for indicating cancer risk remains inconclusive. In our case‐control study consisting 197 leukoplakia 310 oral squamous cell carcinoma (SCC) patients 348 controls, genotype frequencies these loci were determined by PCR‐RFLP method analyzed multiple logistic regression to determine the risks diseases. 2/2 was developing (OR = 1.6, 95% CI 1.1–2.3), whereas...
In vivo analysis of Drosophila melanogaster has enhanced our understanding many biological processes, notably the mechanisms heredity and development. While in mutants been a strength field, analyzing fly cells culture is valuable for cell biological, biochemical whole genome approaches which large numbers homogeneous are required. An efficient genetic method to derive lines using expression an oncogenic form Ras (RasV12) developed. Mutations tumor suppressors, known cause hyperproliferation...
Summary It has been proposed that a constellation of three TP53 polymorphisms (intron 3 16 bp duplication, codon 72 Bst UI, and intron 6 Nci I RFLP at nt 13494) constitute haplotype predictive increased cancer risk. We have estimated the allele frequency these in endogamous Indian ethnic populations from different geographic locations (viz. Iyer south India, Brahmin central India Mahishya eastern India), as well head neck squamous cell carcinoma (HNSCC) patients, ethnically matched normal...
The chronic burden of diabetes on the health individuals has extensive economic and public impact well-being any nation. Strategies in place to curtail growing epidemic challenges mediate their interventions through informative preventive programs place. Research being done overcome these gives hope towards what one can do at an individual level. journey prevention is a long road once limitations healthcare industry are realized.