A5053631838- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 detection and testing
- COVID-19 diagnosis using AI
- Radiation Detection and Scintillator Technologies
- Hepatitis Viruses Studies and Epidemiology
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Hepatitis B Virus Studies
- Virus-based gene therapy research
- Respiratory viral infections research
- Biosensors and Analytical Detection
- Salmonella and Campylobacter epidemiology
- RNA and protein synthesis mechanisms
- Hepatitis C virus research
- Public Health in Brazil
- Aquaculture disease management and microbiota
- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Brazilian Legal Issues
- Advanced biosensing and bioanalysis techniques
- Health, Nursing, Elderly Care
- Social and Political Issues
- Aquaculture Nutrition and Growth
- Animal Disease Management and Epidemiology
- Infection Control and Ventilation
Universitat de Barcelona
2016-2025
Weatherford College
2024
National Research Centre
2024
Ain Shams University
2024
University of Minho
2022-2023
Universidade Santa Cecília
2017-2022
Ação Educativa
2022
Pontifícia Universidade Católica de São Paulo
2022
Hebron University
2019
Vall d'Hebron Hospital Universitari
2017
ABSTRACT A standardized real-time reverse transcription-PCR (RT-PCR) assay has been developed for an accurate estimation of the number genome copies hepatitis virus (HAV) in clinical and shellfish samples. Real-time procedures were based on amplification a fragment highly conserved 5′ noncoding region detection through internal fluorescent probe, including TaqMan beacon chemistries, one- two-step RT-PCR formats. The best performance terms sensitivity reproducibility was achieved by one-step...
The survival of human enteric viruses on several porous (paper and cotton cloth) nonporous (aluminum, china, glazed tile, latex, polystyrene) environmental surfaces has been evaluated. Viruses persisted for extended periods types materials commonly found in institutions domestic environments. stability the was generally influenced by factors such as relative humidity (RH), temperature, type surface contaminated. Overall, hepatitis A virus (HAV) rotavirus (HRV) were more resistant to...
Given the widespread concern but general lack of information over possibility SARS-CoV-2 infection in public transport, key issues such as passenger personal hygiene, efficient air circulation systems, and effective disinfection frequently touched surfaces need to be evaluated educate diminish risk viral transmission we learn live with ongoing pandemic. In this context report on a study involving collection 99 samples taken from inside Barcelona buses subway trains May July 2020. From sample...
Wastewater-based surveillance can be a valuable tool to monitor viral circulation and serve as an early warning system. For respiratory viruses that share similar clinical symptoms, namely SARS-CoV-2, influenza, syncytial virus (RSV), identification in wastewater may allow differentiation between seasonal outbreaks COVID-19 peaks. In this study, these well standard indicators of fecal contamination, weekly sampling campaign was carried out for 15 months (from September 2021 November 2022)...
In 2024, circulating vaccine-derived poliovirus type 2 (cVDPV2) was detected in wastewater samples Finland, Germany, Poland, Spain and the United Kingdom (UK). All strains were genetically linked, but sequence analysis showed high genetic diversity among identified within individual sites countries an unexpected proximity isolates from different countries. Taken together these results, with sequential having tested positive various sites, a broader geographic distribution beyond sampling...
In the present work, we aimed at determining relationship between hepatitis A virus (HAV) numbers in imported frozen coquina clams involved two outbreaks, as well risk for human health. Due to HAV unculturability, a standardized TaqMan real-time reverse transcription-PCR controlling virus/nucleic acid extraction and enzyme efficiencies was employed figure exposure dose responsible cases. were then of infection based on dose-response model echovirus 12. The estimated after consumption lightly...
ABSTRACT A 3-year study involving 2,347 gastroenteritis samples was conducted to determine the prevalence, time distribution, and medical significance of human astrovirus infection in Barcelona, Spain. The overall incidence found be 4.9%. Mixed infections with other enteric agents were detected 17.2% all astrovirus-positive samples. During period, highest reported winter months, although also occurred summer. peak detection rate observed children between 2 4 years age. Overall, HAstV-1 most...
Hepatitis A virus (HAV), the prototype of genus Hepatovirus, has several unique biological characteristics that distinguish it from other members Picornaviridae family. Among these, need for an intact eIF4G factor initiation translation results in inability to shut down host protein synthesis by a mechanism similar picornaviruses. Consequently, HAV must inefficiently compete cellular translational machinery and this may explain its poor growth cell culture. In context virus/cell competition,...
Abstract SARS-CoV-2 was detected in Barcelona sewage long before the declaration of first COVID-19 case, indicating that infection present population imported case reported. Sentinel surveillance wastewater would enable adoption immediate measures event future waves. Article Summary Line genomes occur cases among population.
Water-based epidemiology (WBE) is a valuable early warning tool for tracking the circulation of virus among population, including not only symptomatic patients, but also asymptomatic, presymptomatic, and misdiagnosed carriers, which represent high proportion infected population. In specific case Barcelona, wastewater surveillance anticipated by several weeks original COVID-19 pandemic wave, onset second wave.
Since its first identification in the United Kingdom late 2020, highly transmissible B.1.1.7 variant of SARS-CoV-2 has become dominant several countries raising great concern. We developed a duplex real-time RT-qPCR assay to detect, discriminate, and quantitate variants containing one mutation signatures, ΔHV69/70 deletion, used it trace community circulation Spain through Spanish National Wastewater Surveillance System (VATar COVID-19). The was detected earlier than clinical epidemiological...
Besides nasopharyngeal swabs, monkeypox virus (MPXV) DNA has been detected in a variety of samples such as saliva, semen, urine and fecal samples. Using the environmental surveillance network previously developed Spain for routine wastewater SARS-CoV-2 (VATar COVID-19), we have analyzed presence MPXV from different areas Spain. Samples (n = 312) 24 treatment plants were obtained between May 9 (week 19 2022) August 4 31 2022). Following concentration viral particles by validated aluminum...
One hundred eighty-four serologically confirmed cases of hepatitis A were reported in eastern Spain 1999. matched case-control study implicated imported coquina clams complying with European Union shellfish standards as the source infection; this implication was by detection reverse transcription-PCR virus (HAV) RNA samples. In spite recognized low variability HAV, genetic characterization complete capsid region isolates from patient serum samples revealed existence both synonymous and...
ABSTRACT The number of synonymous mutations per site ( K s ), the nonsynonymous a and codon usage statistic N c ) were calculated for several hepatitis A virus (HAV) isolates. While was similar to those poliovirus (PV) foot-and-mouth disease (FMDV), 1 order magnitude lower. parameter provides information on bias decreases when increases. value in HAV about 38, while PV FMDV, it 53. emergence 22 rare codons front 8 7 FMDV detected. Most conserved P1 region strategically located at carboxy...
Abstract Background In July 2005 an outbreak of acute gastroenteritis occurred on a residential summer camp in the province Barcelona (northeast Spain). Forty-four people were affected among residents and employees. All them had common meal at lunch time 13 (paella, round beef fruit). The aim this study was to investigate foodborne norovirus that which implication food handler demonstrated by laboratory tests. Methods A retrospective cohort designed. Personal or telephone interview carried...
Summary Human noroviruses (NoV) were quantified and characterized in an 18 month survey conducted along the Llobregat river catchment Spain. Sample types included freshwater, untreated treated wastewater drinking water. High NoV genome copy numbers reported, reaching up to 10 6 l −1 9 freshwater raw sewage respectively. In both of samples, GII copies outnumbered those GI, although without significance. All samples semi‐treated water negative for NoV. A clear seasonality occurrence was...