A5023346310- Viral gastroenteritis research and epidemiology
- Hepatitis Viruses Studies and Epidemiology
- Medical Malpractice and Liability Issues
- Patient Dignity and Privacy
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Viral Infections and Immunology Research
- Disaster Response and Management
- Animal Virus Infections Studies
- Chronic Obstructive Pulmonary Disease (COPD) Research
- HIV/AIDS Research and Interventions
- Mesenchymal stem cell research
- Molecular Biology Techniques and Applications
- HIV/AIDS drug development and treatment
- Ethics in Clinical Research
- HIV Research and Treatment
- Virus-based gene therapy research
- Inflammatory Biomarkers in Disease Prognosis
- Cancer-related molecular mechanisms research
- Lung Cancer Treatments and Mutations
- RNA modifications and cancer
- Biosimilars and Bioanalytical Methods
- SARS-CoV-2 and COVID-19 Research
- Bioeconomy and Sustainability Development
- Cancer Genomics and Diagnostics
- Inflammasome and immune disorders
Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública
2020-2023
Instituto de Salud Carlos III
2015-2023
Health Research Institute of the Balearic Islands
2019-2023
Hospital Universitario Son Espases
2019-2023
Centro de Investigación Biomédica en Red de Enfermedades Respiratorias
2009-2023
Centro de Investigación Biomédica en Red
2017-2021
Institut Universitaire de Cardiologie et de Pneumologie de Québec
2010
Centre hospitalier universitaire de Québec
2010
Université Laval
2010
Hospital Universitari Germans Trias i Pujol
2005-2007
ABSTRACT A 3-year study involving 2,347 gastroenteritis samples was conducted to determine the prevalence, time distribution, and medical significance of human astrovirus infection in Barcelona, Spain. The overall incidence found be 4.9%. Mixed infections with other enteric agents were detected 17.2% all astrovirus-positive samples. During period, highest reported winter months, although also occurred summer. peak detection rate observed children between 2 4 years age. Overall, HAstV-1 most...
A method based on infection of CaCo-2 cultured cell monolayers (CC) and reverse transcription-PCR (RT-PCR) was developed for the specific detection infectious astrovirus. The procedure validated by titrating poliovirus stocks in parallel cells determining most probable number cytopathogenic units culture subsequent RT-PCR (CC-RT-PCR). CC-RT-PCR then employed to measure persistence astrovirus suspended dechlorinated tap water. After 60 days, decay infectivity 2 log at 4 +/- 1 degrees C 3.2 20...
ABSTRACT The presence of rotavirus strains in sewage samples from Cairo, Egypt (November 1998 to October 1999), and Barcelona, Spain December 2002), was investigated by using a generic molecular detection method based on amplification VP6 gene fragment. Overall, 85.7 66.9% the Cairo respectively, were positive. Positive characterized further, VP7 VP4 genotypes determined. Although 30% positive G untypeable, distribution types 69.6% G1, 13% G3, 8.7% G4, G9. percentage untypeable much higher...
ABSTRACT The persistence of human astroviruses dried on representative porous (paper) and nonporous (china) surfaces was investigated. Long-term astrovirus survival fomites monitored by an integrated cell culture-reverse transcription-PCR procedure. Viruses were applied to inanimate in the presence absence fecal material, their assayed at 4 20°C with high relative humidity. Astroviruses exhibited a notable when materials, particularly low temperature. Short-term compared that other enteric...
Molecular methods for the detection and typing of hepatitis A virus (HAV) strains in sewage were applied to determine its distribution Cairo Barcelona. The study revealed occurrence different patterns endemicity each city. circulating characterized, whether or Barcelona, genotype IB. effects a child vaccination programme increase immigrant population on overall Barcelona evaluated. While contributed significant decrease number clinical cases, huge recent immigration flow has probably been...
By the end of December 2000, epidemiological system ‘Alert’ Public Health Institute in Tirane reported an outbreak acute gastroenteritis. The involved children and rural area. In total, 2722 were seen Hospital 982 (56·4%) treated for age group with highest morbidity was 0–5 years (89·7%), followed by 6–9 (6·2%) 10–15 groups (4·1%). distribution gastroenteritis cases, which occurred along same water system, suggests a waterborne origin. nucleic acid amplification confirmed co-circulation...
Three different studies are reported concerning the environmental pollution caused by viruses in Albania. The first study describes an outbreak of gastroenteritis capital city, involving 2,722 children attending Paediatric Unit Tirana Hospital. age group with highest morbidity was 0-5 years, 89.5%; no fatalities were recorded during outbreak. Rotavirus detected 26/28 faecal samples RT-PCR, although astrovirus, adenovirus and calicivirus also present. second hepatitis A virus city Lac. Two...
COPD is a chronic lung disease that affects millions of people, declining their function and impairing life quality. Despite years research drug approvals, we are still not capable halting progression or restoring normal function. Mesenchymal stem cells (MSC) with extraordinary repair capacity, MSC-based therapy brings future hope for treatment, although the best source route administration unclear. MSC from adipose tissue (AD-MSC) represents an option autologous treatment; however, they...
It is known that pro-inflammatory cytokines suppress in vitro the gene expression and protein production of erythropoietin (Epo). We hypothesized systemic inflammation patients with chronic obstructive pulmonary disease (COPD) may influence Epo production, particularly during episodes exacerbation (ECOPD) where an inflammatory burst to occur.We compared plasma levels high-sensitivity (hs) C-reactive (hsC-RP) hospitalized because ECOPD (n = 26; FEV(1): 48 +/- 15% predicted), clinically stable...
Procedures for the detection of astroviruses in wastewater samples have been developed and evaluated. Following these methodologies, we investigated occurrence from three different sewage treatments plants located Southern France two Barcelona area. Some positive were genotyped by analysis a fragment ORF1a restriction length polymorphism (RFLP) with endonuclease DdeI. The amplimers generated contain several sites DdeI endonuclease, being number location between strains.
Deletions, insertions, and amino acid substitutions in the beta3-beta4 hairpin loop-coding region of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) have been associated with resistance to nucleoside RT inhibitors when appearing combination other mutations RT-coding region. In this work, we measured vivo fitness HIV-1 variants containing a deletion 3 nucleotides affecting codon 69 (Delta69) viral as well replication capacity (RC) ex series recombinant carrying an...
Abstract Aiming to increase the reproducibility of biomedical research results, biobanks obtain human tissues highest quality and carry out different storage methods adapted needs analytical technique be performed by researchers. However, there is much controversy little data concerning real impact stabilization on tissue quality, integrity functionality derived biomolecules. The influence four [RNA later (RNL), snap freezing (SF), using Optimal Cutting Tissue compound (SF-OCT)...
Large gastroenteritis outbreaks associated with astroviruses are being reported increasing frequency suggesting that astrovirus may be an important agent of epidemic acute non-bacterial in children and adults. In this study, a procedure, based on infection CaCo-2 cell monolayers followed by reverse-transcription polymerase chain reaction, was developed to ascertain the persistence infectious human tap marine water at 4±1°C 20±1°C. The adequacy methodology for monitoring decay fastidious...
Large gastroenteritis outbreaks associated with astroviruses are being reported increasing frequency suggesting that astrovirus may be an important agent of epidemic acute non-bacterial in children and adults. In this study, a procedure, based on infection CaCo-2 cell monolayers followed by reverse-transcription polymerase chain reaction, was developed to ascertain the persistence infectious human tap marine water at 4±1°C 20±1°C. The adequacy methodology for monitoring decay fastidious...
<b>Introduction:</b> COPD is a chronic lung disease that affects millions of people declining their function and impairing life quality. Despite years research drug approvals, we are still not capable halting progression or restoring normal function. Mesenchymal stem cells (MSC) pluripotent with extraordinary repair capacity, MSC based therapy brings future hope for treatment, although the best source route administration unclear. from adipose tissue (AD-MSC) represents an option autologous...
Hepatitis A virus (HAV) infection is the leading cause of viral hepatitis throughout world (1). HAV mainly propagated via fecal-oral route (2), and waterborne (3) foodborne (4–8) outbreaks disease have been reported.
ABSTRACT Human immunodeficiency virus type 1 (HIV-1) genotyping assays have come to be widely used for monitoring antiretroviral drug resistance. We report a case in which primer-template mismatches during nested PCR-based amplification biased the composition of original viral population sample, magnifying distinct minority HIV-1 population. This observation might help explain some unexpected genotypes.
<b>Introduction:</b> At present there is no clear evidence of the involvement mesenchymal stem cells (MSC) in certain pathologies lung like idiopathic pulmonary fibrosis (IPF). The objective study was to isolate and characterize MSC from patients with without IPF evaluate their distinct properties hypothetical contribution IPF. <b>Methods Results:</b> were isolated lungs control donors (MSC-D) (MSC-IPF). Cells grown characterized Mesenchymal SC analysis kit (BD) differentiation performed...