More than 20 unrelated proteins can form amyloid fibrils in vivo which are related to various diseases, such as Alzheimer's disease, prion and systematic amyloidosis. Amyloid an ordered protein aggregate with a lamellar cross-beta structure. Enhancing clearance is one of the targets therapy these amyloid-related diseases. Although there debate on whether toxicity due amyloids or their precursors, research degradation may help prevent alleviate In this study, we explored amyloid-degrading...

Several Rhodopseudomonas palustris strains have been isolated from rice paddy fields in Taiwan by combining the Winogradsky column method and molecular marker detection. These isolates were initially screened employing seed germination seedling vigor assays to evaluate their potential as inoculants. To fulfill demand present farming system for reducing application of chemical fertilizers, we assessed plant growth-promoting effects R. YSC3, YSC4, PS3 inoculants on Brassica rapa chinensis...

Enterococcus faecalis is a significant pathogen in healthcare settings and frequently resistant to multiple antibiotics. This resistance compounded by its ability form biofilms, dense bacterial communities that are challenging eliminate via standard antibiotic therapies. As such, targeting biofilm formation considered viable strategy for addressing these infections. study assessed the effectiveness of surfactin, cyclic lipopeptide biosurfactant synthesized Bacillus subtilis natto NTU-18,...

On the basis of genomic sequence Bacillus subtilis 168, two β-glucosidase genes ( bglH and yckE) from B. natto, which has been reported to have high isoflavone glucoside-hydrolyzing activity, were cloned overexpressed in E. coli M15. The temperature for optimal p-nitrophenyl-β- d-glucoside hydrolyzing activity both enzymes was between 37 45 °C, but BglH had a higher thermal stability than YckE. Both showed at pH 6.0, YckE stable over wider range BglH. Recombinant inhibited 73%, 63%, 43% by...

We investigated the anticancer effects of Antrodia cinnamomea , a medicinal mushroom from Taiwan, on A549 human lung cancer cells using ethyl acetate extract submerged culture filtrates. Our results showed that 2,3‐dimethoxy‐5‐methyl‐1,4‐benzoquinone (coenzyme Q0; CoQ0) derived A. filtrates has activity. CoQ0 treatment reduced viability A549, HepG2, and SW480 cell lines. Furthermore, induced reactive oxygen species (ROS) generation apoptosis in cells, which was inhibited by antioxidant...

Antrodia cinnamomea, a well-known traditional medicine used in Taiwan, is potent anticancer drug for colorectal cancer, but the upstream molecular mechanism of its effects remains unclear. In this study, A. cinnamomea extracts showed cytotoxicity HCT116, HT29, SW480, Caco-2 and, Colo205 cancer cells. Whole-genome expression profiling HCT116 cells was performed. upregulated endoplasmic reticulum stress marker CHOP and downstream gene TRB3. Moreover, dephosphorylation Akt mTOR as well...

ABSTRACT Medium- and long-chain 1-alkanol α,ω-alkanediols are used in personal care products, industrial lubricants, as precursors for polymers synthesized medical applications. The production of by alkane hydroxylation primarily occurs at high temperature pressure using heavy metal catalysts. However, bioproduction has recently emerged a more economical environmentally friendly alternative. Among monooxygenases, CYP153A from Marinobacter aquaeolei VT8 (CYP153A M.aq ; the strain is also...

Agarwood, the resinous wood in heartwood of Aquilaria trees, has been used as incense traditional Chinese medicine for its sedative, aphrodisiac, carminative, and anti-emetic effects. Grading agarwood is usually based on physical properties. Therefore, it important to develop analytic methods judgment grading agarwood. Here, we created a headspace (HS) preheating system that combined with gas chromatography-mass spectrometry (HS GC-MS) analyze chemical constituents smoke produced by Incense...

Searching for new antimicrobials is a pressing issue to conquer the emergence of multidrug-resistant (MDR) bacteria and fungi. Antimicrobial peptides (AMPs) usually have antimicrobial mechanisms different from those traditional antibiotics bring hope in discovery antimicrobials. In addition activity, stability target selectivity are important concerns decide whether an peptide can be applied vivo. Here, we used simple de novo designed peptide, pepD2, which contains only three kinds amino...

Plant diseases are important issues in agriculture, and the development of effective environment-friendly means disease control is crucial highly desired. Antimicrobial peptides (AMPs) known as potential alternatives to chemical pesticides because their potent broad-spectrum antimicrobial activity they have no risk, or only a low developing chemical-resistant pathogens. In this study, we designed series amphipathic helical with different spatial distributions positive charges found that had...

Lignans, a class of dimeric phenylpropanoid derivative found in plants, such as whole grains and sesame flax seeds, have anticancer activity can act phytoestrogens. The lignans secoisolariciresinol matairesinol be converted the mammalian proximal colon into enterolactone enterodiol, respectively, which reduce risk breast cancer. To establish an efficient bioconversion system to generate from pinoresinol, genes encoding pinoresinol-lariciresinol reductase (PLR) dehydrogenase (SDH) were cloned...

Enterococcus faecalis, a member of the commensal flora in human gastrointestinal tract, has become threatening nosocomial pathogen because it developed resistance to many known antibiotics. More concerningly, gene-carrying E. faecalis cells may transfer antibiotic resistance-free through their unique quorum sensing-mediated plasmid system. Therefore, we investigated role probiotic bacteria frequency pCF10 populations mitigate spread resistance. Bacillus subtilis subsp. natto is strain...

Five new triterpene acids, mesonaic acids A–C (1–3), 2α,3α,19α-trihydroxy-24-nor-4(23),12-oleanadien-28-oic acid (4), and 3α,19α,22α-trihydroxy-2-oxo-12-ursen-28-oic (5), 10 known compounds (6–15) were isolated from a methanolic extract of Mesona procumbens. Triterpenes 1–3 possess unusual hexacyclic skeletons with 13α,27-cyclopropane ring. Regarding their anti-inflammatory activity, 1–3, 6, 7 inhibited NO production EC50 values lower than 15 μM, which better that the positive control...

Abstract Grey mullet ( Mugil cephalus ) aquaculture is economically vital due to the high value of its roe. However, it faces significant risks from disease outbreaks, particularly Nocardia seriolae . Current reliance on antibiotics has drawbacks, highlighting potential probiotics as a promising alternative. Despite this, no studies have focused effects and mechanisms in prevention treatment grey mullet. This study, therefore, investigates efficacy enhancing resistance promoting growth Three...

The hairy root, a specialized plant tissue that emerges from cell transformed with transfer DNA (T-DNA) Agrobacterium rhizogenes, can be used to study root biology and utilized in biotechnological applications. rol genes are known participate the generation of roots; however, means by which contribute initiation maintenance roots remains largely unknown. We demonstrated tobacco lacking either rolB or rolC exhibited fewer branch lost their growth ability long-term subculture. Additionally,...

Enterococcus faecalis is considered a leading cause of hospital-acquired infections. Treatment these infections has become major challenge for clinicians because some E. strains are resistant to multiple clinically used antibiotics. Moreover, the presence biofilms can make with more difficult eradicate current antibiotic therapies. Thus, our aim in this study was investigate effects probiotic derivatives against biofilm formation. Bacillus subtilis natto strain isolated from Japanese...