A5066471003- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Hippo pathway signaling and YAP/TAZ
- Planarian Biology and Electrostimulation
- Advanced Fluorescence Microscopy Techniques
- Axon Guidance and Neuronal Signaling
- Protein Kinase Regulation and GTPase Signaling
- Fibroblast Growth Factor Research
- Caveolin-1 and cellular processes
- Galectins and Cancer Biology
- Cell Image Analysis Techniques
- Protein Tyrosine Phosphatases
- Maritime Transport Emissions and Efficiency
- Cell Adhesion Molecules Research
- Invertebrate Immune Response Mechanisms
- 3D Printing in Biomedical Research
- Reservoir Engineering and Simulation Methods
- Oil Spill Detection and Mitigation
- Developmental Biology and Gene Regulation
- Cancer Cells and Metastasis
Novo Nordisk Foundation
2019-2024
University of Copenhagen
2019-2022
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2019-2020
Universidade do Porto
2014-2019
Universidade Federal do Rio de Janeiro
2011
Abstract During embryonic development, regeneration, and homeostasis, cells have to migrate physically integrate into the target tissues where they ultimately execute their function. While much is known about biochemical pathways driving cell migration in vivo, we are only beginning understand mechanical interplay between migrating surrounding tissue. Here, reveal that multiciliated precursors Xenopus embryo use filopodia pull at vertices of overlying epithelial sheet. This pulling...
Abstract During tissue development and regeneration, cells interpret exert mechanical forces that are challenging to measure in vivo. Therefore, stress inference algorithms have emerged as powerful tools estimate stresses. However, how incorporate dynamics effectively into the remains elusive. Here, we present ForSys, a Python-based software estimates intercellular stresses intracellular pressures using time-lapse microscopy. We validated ForSys silico vivo well-characterized mucociliary...
Apical-basal polarity is a common trait that underlies epithelial function. Although the asymmetric distribution of cortical proteins works in functioning equilibrium, it also retains plasticity to accommodate cell division, during which basolateral determinant Lgl released from cortex. Here, we investigated how restores its localization maintain integrity dividing epithelia. We show cytoplasmic reloaded cortex at mitotic exit Drosophila depends on protein phosphatase 1, dephosphorylates...
Apical-basal polarity underpins the formation of epithelial barriers that are crucial for metazoan physiology. Although apical-basal is long known to require basolateral determinants Lethal Giant Larvae (Lgl), Discs Large (Dlg) and Scribble (Scrib), mechanistic understanding their function limited. Lgl plays a role as an aPKC inhibitor, but it remains unclear whether also forms complexes with Dlg or Scrib. Using fluorescence recovery after photobleaching, we show does not form immobile at...
Phosphorylation and dephosphorylation of protein tyrosine residues constitutes a major biochemical regulatory mechanism for the cell. We report transient increase in total phosphorylation Aedes aegypti head during first days after emergence from pupal stage. This correlates with an initial reduction phosphatase (PTP) activity. Similarly, phosphotyrosine (pTyr)-containing bands are seen extracts prepared both male female heads spread among variety structures including antennae, proboscis...
Atmospheric methane (CH4) concentrations have more than doubled since the beginning of industrial age, making CH4 second most important anthropogenic greenhouse gas after carbon dioxide (CO2). The oil and (O&G) sectors are one major sources accounting for 22% global emissions. METHANE-To-Go Africa (MTGA) scientific aircraft campaign in September 2022 was conducted as part UNEP’s International Methane Emissions Observatory (IMEO). During campaign, we first large scale...
Abstract Apical-basal polarity underpins the formation of specialized epithelial barriers that are critical for metazoan physiology. Although apical-basal is long known to require basolateral determinants Lethal Giant Larvae (Lgl), Discs Large (Dlg) and Scribble (Scrib), mechanistic understanding their function limited. Lgl plays a role as an aPKC inhibitor, but it remains unclear whether also forms complex with Dlg or Scrib. Using fluorescence recovery after photobleaching, we show does not...
ABSTRACT During embryonic development, regeneration and homeostasis, cells have to physically integrate into their target tissues, where they ultimately execute function. Despite a significant body of research on how mechanical forces instruct cellular behaviors within the plane an epithelium, very little is known about interplay at interface between migrating surrounding tissue, which has its own dynamics, architecture identity. Here, using quantitative in vivo imaging molecular...
During embryonic development, regeneration and homeostasis, cells have to physically integrate into their target tissues, where they ultimately execute function. Despite a significant body of research on how mechanical forces instruct cellular behaviors within the plane an epithelium, very little is known about interplay at interface between migrating surrounding tissue, which has its own dynamics, architecture identity. Here, using quantitative in vivo imaging molecular perturbations,...