A5009094314- RNA Research and Splicing
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- Cellular Mechanics and Interactions
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Developmental Biology and Gene Regulation
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
- Zebrafish Biomedical Research Applications
- Pluripotent Stem Cells Research
- Genetics, Aging, and Longevity in Model Organisms
- RNA regulation and disease
- Circular RNAs in diseases
- Animal Genetics and Reproduction
- Microtubule and mitosis dynamics
Max Planck Institute of Molecular Cell Biology and Genetics
2019-2023
University of Lausanne
2022-2023
McGill University
2016
McGill University Health Centre
2010
Centre Hospitalier Universitaire Sainte-Justine
2010
Cytoplasmic poly(A)-binding proteins (PABPs) link mRNA 3′ termini to translation initiation factors, but they also play key roles in regulation and decay. Reports from mice, zebrafish Drosophila further involved PABPs microRNA (miRNA)-mediated silencing, through seemingly distinct mechanisms. Here, we implicate the two Caenorhabditis elegans (PAB-1 PAB-2) miRNA-mediated elucidate their mechanisms of action using concerted genetics, protein interaction analyses, cell-free assays. We find that...
MicroRNAs (miRNAs) impinge on the translation and stability of their target mRNAs, play key roles in development, homeostasis disease. The gene regulation mechanisms they instigate are largely mediated through CCR4–NOT deadenylase complex, but molecular events that occur mRNAs poorly resolved. We observed a broad convergence interactions germ granule P body mRNP components AIN-1/GW182 NTL-1/CNOT1 Caenorhabditis elegans embryos. show miRISC progressively matures mRNA from scanning form into...
In the cytoplasm, filamentous actin (F-actin) plays a critical role in cell regulation, including migration, stress fiber formation, and cytokinesis. Recent studies have shown that filaments form nucleus are associated with diverse functions. Here, using live imaging of an F-actin-specific probe, superfolder GFP-tagged utrophin (UtrCH-sfGFP), we demonstrated dynamics nuclear zebrafish (Danio rerio) embryos. early embryos up to around high stage, UtrCH-sfGFP increasingly accumulated nuclei...
ABSTRACT The localization of transcriptional activity in specialized transcription bodies is a hallmark gene expression eukaryotic cells. How proteins the machinery come together to form such bodies, however, unclear. Here, we take advantage two large, isolated, and long-lived that reproducibly during early zebrafish embryogenesis, characterize dynamics body formation. Once formed, these are enriched for initiating elongating RNA polymerase II, as well factors Nanog Sox19b. Analyzing events...
Abstract In the cytoplasm, filamentous actin (F-actin) plays a critical role in cell regulation, including migration, stress fiber formation, and cytokinesis. Recent studies have shown that filaments form nucleus are associated with diverse functions. Here, using live imaging of an F-actin-specific probe, superfolder GFP-tagged utrophin (UtrCH-sfGFP), we demonstrated dynamics nuclear zebrafish ( Danio rerio ) embryos. early embryos up to around high stage, UtrCH-sfGFP increasingly...