A5044032359- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Reproductive Biology and Fertility
- Pancreatic function and diabetes
- Pluripotent Stem Cells Research
- Hematopoietic Stem Cell Transplantation
- Periodontal Regeneration and Treatments
- Sperm and Testicular Function
- Biomedical Ethics and Regulation
- Electrospun Nanofibers in Biomedical Applications
- Renal and related cancers
- 3D Printing in Biomedical Research
- Organ Transplantation Techniques and Outcomes
- Diabetes Management and Research
- Reproductive Health and Technologies
- Dental Trauma and Treatments
- Cannabis and Cannabinoid Research
- Xenotransplantation and immune response
- Cancer Cells and Metastasis
- Organ and Tissue Transplantation Research
- Organ Donation and Transplantation
- Ovarian function and disorders
- Chemical and Physical Properties in Aqueous Solutions
- Viral Infectious Diseases and Gene Expression in Insects
- Electrohydrodynamics and Fluid Dynamics
Indiana University – Purdue University Indianapolis
2013-2024
Indiana University School of Medicine
2012-2022
Marian University - Indiana
2020-2022
Indiana University
2001-2020
University School
2019
Cook General BioTechnology (United States)
2007-2016
Cook Research (United States)
2015-2016
International Society for Cellular Therapy
2016
Toho University
2015
Toho University Ohashi Medical Center
2015
Recent studies have shown that mesenchymal stem cells (MSC) with the potential for cell-mediated therapies and tissue engineering applications can be isolated from extracted dental tissues. Here, we investigated collection, processing, cryobiological characteristics of MSC human teeth processed under current good practices (cGTP). Viable pulp-derived (DPSC) cultures were 31 40 examined. Of eight DPSC examined more thoroughly, all expressed appropriate cell surface markers underwent...
Dental pulp stem cells (DPSCs) have drawn much interest for the regeneration of mineralized tissues, and several studies compared DPSCs to bone marrow-derived mesenchymal (BMMSCs). However, conflicting results, possibly due donor-associated variability, been published regenerative potential is currently unclear. In present study we sought address this problem using a donor-matched experimental design robustly compare biological properties BMMSCs. All experiments were performed isolated from...
There has been a great deal of scientific interest recently generated by the potential therapeutic applications adult stem cells in human care but there are several challenges regarding quality and safety clinical number these relate to processing banking ex-vivo. As trials variety used regenerative therapy increases, remains primary concern. This inspired many nations formulate guidelines standards for cell collection, processing, testing, banking, packaging distribution. Clinically...
Abstract Endometrial Regenerative Cells (ERC) are a population of mesenchymal-like stem cells having pluripotent differentiation activity and ability to induce neoangiogenesis. In vitro animal studies suggest ERC immune privileged in certain situations actively suppress ongoing responses. this paper we describe the production clinical grade initial safety experiences 4 patients with multiple sclerosis treated intravenously intrathecally. The case longest follow up, more than one year,...
Cellular therapy for spinal cord injury (SCI) is overviewed focusing on bone marrow mononuclear cells, olfactory ensheathing and mesenchymal stem cells. A case made the possibility of combining cell types, as well allogeneic use. We report 29 year old male who suffered a crush fracture L1 vertebral body, lacking lower sensorimotor function, being score ASIA scale. Stem comprised intrathecal administration umbilical blood ex-vivo expanded CD34 matrix MSC was performed 5 months, 8 14 months...
Coral reefs are experiencing unprecedented degradation due to human activities, and protecting specific reef habitats may not stop this decline, because the most serious threats global (i.e., climate change), local. However, ex situ preservation practices can provide safeguards for coral conservation. Specifically, modern advances in cryobiology genome banking could secure existing species genetic diversity until genotypes be introduced into rehabilitated habitats. We assessed feasibility of...
The transplantation of placental/cord blood-derived HPC (e.g., CD34+ cells) has become a useful treatment for broad spectrum malignant and nonmalignant diseases. ability to cryopreserve this cell type with high efficiency adds considerable flexibility cord blood transplantation. purpose study was develop an understanding the fundamental cryobiologic factors these cells, including osmotic/permeability characteristics, use theoretical approach optimize freezing procedures. To that end,...
Successful cryopreservation requires cells to tolerate volume excursions experienced during permeating cryoprotectant equilibration and cooling warming. However, prior studies have demonstrated that mouse spermatozoa are extremely sensitive osmotically induced changes. A series of three experiments were conducted 1) test the efficacy two commonly used extender media components, egg yolk (EY) skim milk (SM), in broadening osmotic tolerance limits (OTL) ICR B6C3F1 murine spermatozoa; 2)...
Patients with congestive heart failure (CHF) that are not eligible for transplantation have limited therapeutic options.Stem cell therapy such as autologous bone marrow, mobilized peripheral blood, or purified cells thereof has been used clinically since 2001.To date over 1000 patients received cellular part of randomized trials, the general consensus being a moderate but statistically significant benefit occurs.Therefore, one important next steps in field is optimization.In this paper we...
Current mammalian embryo cryopreservation protocols typically employ an interrupted slow freezing (ISF) procedure. In general, ISF consists of initial cooling, which raises the extracellular solute concentration, and results in cell dehydration. Permeating cryoprotective agents (CPAs), such as dimethyl sulfoxide (DMSO), are included medium to protect cells against high concentrations. As this procedure continues, cooling is terminated at intermediate temperature (Tp), followed by plunging...